We narrowed to 1,073 results for: TOR

...of the most compact genome editors to date. We have developed multiple vector versions for TnpB-mediated...especially valuable for regulatory approval and public acceptance. Recently, viral vectors have been successfully...Practical Guide: Using pK-TnpB1 and pK-TnpB2 Vectors The vectors pK-TnpB1 and pK-TnpB2 are designed for plant...the digested vector. Step 4: Ligation Ligate the annealed oligos into the digested vector using your ...Precise plant genome editing using base editors and prime editors. Nature Plants, 7(9), 1166–1187. https...blog post was written by Dr. Kutubuddin Molla, investigator at ICAR-Central National Rice Research Institute...efficient delivery into cells, particularly via viral vectors. While viral delivery is a highly effective method...

..., Word docs, and the like is likely stored in online file storage tools like Dropbox, Box, One Drive, ...commonly used online file storage tools, as illustrated in this video, and to data stored on institutional and...source data repositories Over the past decade or so a number of general purpose repositories have been ... in the repository after the deposit has been made. It’s possible to associate the depositor’s ORCID ID...value the repository integrations, because when RSpace documents are deposited into the repositories, links...research; and (3) connectivity to open source data repositories. To illustrate this, let’s take a look at the...researchers and PIs, science librarians and data curators, IT managers, the commercialization office, the...

...HiBiT-TP53 Fusion Vector at Addgene! Following extracellular RNA editors Tracking factors that utilize exosomal... to track epidermal growth factor receptor (EGFR), a transmembrane receptor tyrosine kinase essential ...EGFR-HiBiT Fusion Vectors at Addgene! Measuring messengers: growth factors Growth factors present unique ...measurements. Find CFTR-HiBiT Fusion Vector at Addgene! Real-time tracking of receptor internalization Work conducted...This method offered kinetic, non-invasive monitoring of receptor dynamics at the cell surface. Find HiBiT-EGFR...interactions with co-receptors (like NRP1) and by endocytic trafficking of the receptor. Peach et al. (2021...to subtle changes in co-receptor dynamics, including NanoBRET assays to monitor the binding of fluorescently-labeled...

...communication. Viral vectors and the nervous system With the advent of viral vector technology, new methods...been used for tracing and visualizing motor, visual, and auditory neuronal circuitry in vivo. A variant... at Addgene). AAV vectors: A platform for delivering neuronal tracers AAV vectors have been widely used...and Mooney, R. (2017). Identification of a motor to auditory pathway important for vocal learning. Nat ...genome is a single-stranded DNA molecule and AAV vectors are replication deficient (i.e. they cannot easily... low immunogenicity and cellular toxicity. AAV vectors can be equipped with strong promoters for high ... is often made Cre-dependent. If Cre-dependent vectors are used in transgenic animals that express Cre...

... edit (or edits) engages the prime editor protein. The prime editor consists of a Cas9 nickase fused to...range of specialized prime editors (PEs). PE6a and PE6b are small prime editors with RT domains derived ...genome editing methods using nucleases and base editors have the potential to correct only a minority of...the edited strand. The first iteration of prime editor simply fused the wild-type Moloney Murine Leukemia.... We’ll summarize the major ones in Addgene’s repository later in the post. Advantages of prime editing... constrained by PAM sequence location The prime editor extends the reach of CRISPR genome editing, as ...either DNA strand, many previously developed base editors with a <8 base-pair editing window cannot edit ...

... Depositor Collections Cell Migration Consortium (CMC) Plasmids...Cell Migration Consortium and Addgene. Member laboratories of the Cell Migration Consortium have deposited...for cell migration research at Addgene. CMC investigators who have deposited plasmids include: Joan Brugge...Francisco Browse Plasmids ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

...ingredients: the lentiviral packaging vector, a transfer vector including the gene of interest, and the...3rd-generation transfer vectors apart? A3: Addgene defines 2nd- or 3rd-generation transfer vectors based on whether...between 2nd- and 3rd-generation vectors? What does this mean for transfer vectors? A4: The lentiviral packaging...largely based upon the vector system used and the transgene insert encoded by the vector." The table below ...additional information or clarification on these vectors. Read on to find the answers to our most frequently...questions. This useful resource from Addgene Depositor (and lentiviral expert) Didier Trono's lab webpage...years based on HIV-1; however, the 1st-generation vectors are never used as they pose too much of a biosafety...

...large size of Cas9 activators, which largely precludes delivery by adeno-associated vectors (AAV). This issue...Suntag” Cas9 activator that recruits up to twenty copies of the p65-HSF1 transcriptional activator to each ...found here. Neuron-optimized viral vectors Alternatively, Cas9 activators can be delivered to the CNS by ...mCherry (vector 1) with neuron-specific expression of dCas9-VPR (Figure 2), a popular Cas9 activator developed...Patrick Miller-Rhodes, a Ruth L. Kirschstein NRSA Predoctoral Fellow at University of Rochester Medical Center...including transgenic mice, neuron-optimized viral vectors, and high-throughput screening approaches. Here...and engineered combinations of transcriptional activators to induce gene transcription at genomic sites...

... viral vector preparations. Addgene has used this method to generally validate our viral vector preparation...of an Addgene AAV viral vector preparation after negative staining. Empty vector particles can be identified...darker than full vector particles. This image shows that the vast majority of the vectors consist of full...affected by many experimental factors. To list some complicating factors for quantifying AAV: qPCR efficiency...which is a factor in AAV titration since the starting template is the purified viral vector solution (...feasible qPCR strategy for titrating AAV vectors." Medical science monitor basic research19 (2013): 187. PubMed...many ways to quantify the composition of a viral vector solution, and generally these methods measure different...

... plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects genome... Depositor Collections IGVF Consortium Impact of Genomic Variation...collaborative program that brings together teams of investigators who will use state-of-the-art experimental and...plasmid tools through Addgene. ID Plasmid Description Vector Type PI Publication Additional Resources The IGVF...

...this environment produce growth factor signals such as tumor growth factor beta (TGFβ) and IL-10 that suppress... colony stimulating factor 1 (CSF-1). Additionally, tumor cells recruit regulatory T cells (T-regs) into... of other effector immune cells. TGFβ is also known to change CD4+ Th cells into regulatory cells to suppress...to inhibit expression of co-stimulatory molecules needed for T cell effector function. Cancer cells also... mice. Chimeric antigen receptors T cells producing chimeric antigen receptors (CARs) on their surfaces...contributed by guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University Cancer is one...1. Eliminate - Latent tumors are recognized by effector cells of the immune system - cytotoxic T lymphocytes...

...and feature information given by our depositors. Many depositors also upload their own detailed plasmid...research, and helps requestors use the plasmid in their own labs. We highly encourage depositors to provide plasmid...of the Principal Investigator (PI) in whose lab the construct was made. The depositor name links to a ...starting vector that the depositing lab modified to create their new plasmid.  The Addgene Vector Database... modifying a vector previously deposited with Addgene, you can list the original vector’s plasmid number...also like to store plasmids that need extra stability, such as some of our viral vectors, in NEB Stable... used in your research! Sequence information: Depositors may provide full or partial sequence information...

...will aid neuroscientists in studying circuits in laboratory animals and human tissue specimens. These technologies...rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons...lines or co-infection with Cre dependent viral vectors. Jonathan Ting Viviana Gradinaru 163509-PHPeB CN1839...CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for...BGHpA DLX2.0 (3xCore version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain...

...Agrobacterium binary vectors Vectors designed for use in Agrobacterium are termed binary vectors because they .... coli. Addgene depositor Indu Maiti has created a new and versatile binary Ti vector for both transient...pSIM24! To build a better binary vector, Sahoo et al. started with a vector commonly used by their lab, the...applications in plants. This smaller, easily customizable vector functions in multiple species, including tobacco...coli. Like plasmids used in other systems, binary vectors are cloned and prepped in E. coli. However, they...co-cultivation with the target plant species. Thus, binary vectors contain two origins of replication, one each for... be transferred to the plant, similar to viral vectors’ terminal repeats. It’s a lot of information to...

...Level 1 vector with a promoter and terminator flanking the CDS, and a Level 2 multigene vector with two...interchangeable between different laboratories. Therefore, choosing acceptor vectors and parts with the adequate...carrying each part, ‘vector’ refers to acceptor plasmids, and the ‘amount of vector’ and the ‘molar ratio... questions like these will determine which acceptor vectors and parts you will be using during the different...C-terminal tag; TER, terminator. Created with BioRender.com. The Sainsbury Laboratory Golden Gate Cloning...You can input the sequences of the parts and acceptor vectors, as well as the restriction enzymes, and it...purposes. But what now? MoClo really simplifies vector construction by combining restriction and ligation...

... India, and Mexico. Table 2: Addgene distributors Distributor Partnership Start Date Country Served... with a distributor. Arrows indicate the year we entered into an agreement with a distributor for that... materials in our repository. We started to increase accessibility to our repository by making it easier...Addgene opened in 2004, we focused on creating a repository that made it easy to share plasmids, both for...celebration, we thought it only fitting to share some stories of ways we’ve turned the available into the accessible...to — so far, we’ve shipped materials from our repository to an amazing 111 countries (Figure 1)! But while...would benefit from a third-party “helper”: a distributor who, among other things, could ensure our packages...

...Repressible: An activator protein is bound, turning transcription ON. When a repressor binds the activator protein...introduced the second generation QF2 activators. These activators solve the problem of broad QF expression-induced...transcription factor cannot drive transcription from the QUAS promoter, and the QF transcription factor cannot...guide to working with Drosophila Terminators and PolyA Signals FLEx Vectors Resources at Addgene.org Tetracycline...the binding of RNA polymerase and transcription factors. Since the promoter region drives transcription...protein, the activator protein cannot bind the promoter sequence anymore and transcription is turned OFF....protein, the repressor protein can bind to the operator. The bound repressor then prevents transcription...

...directly to the acceptor fluorophore--think of it as a virtual photon 3. Relaxation of the acceptor fluorophore... the acceptor In order for FRET to occur several constraints must be met. The donor and acceptor fluorophores...photons) from the excited donor fluorophore to the acceptor fluorophore. The typical steps in FRET are: 1....fluorophore overlaps with the excitation spectrum of the acceptor fluorophore. Otherwise the energy transferred ... from the donor will not be able to excite the acceptor. In addition to sufficient spectral overlap, the...efficiency of FRET can be measured for a given donor-acceptor pair and a change in FRET efficiency correlates...Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells." (...

...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. Figure 1: Overview of ...

...RTK signaling Receptor tyrosine kinases (RTKs) are a major class of signaling receptors, but the options... for monitoring RTK signaling.  Figure 4: Design of pYtag fluorescent biosensors for monitoring RTK activation...of fluorescent potassium indicators with a range of binding affinities (Torres Cabán et al. 2022): KRaION1...one of the loop regions of any G-protein-coupled receptor (GPCR) recognizing a specific ligand (Ino et al...intracellular loop of various G-protein-coupled receptors. Image adapted from Ino et al. 2022, under CC-...doi: 10.1038/s41592-022-01597-x. New glutamate indicator iGluSnFR3 Improving on the glutamate sensor iGluSnFR...PDGFR.codonopt    Aggarwal A, et al. Glutamate indicators with improved activation kinetics and localization...