We narrowed to 728 results for: STO
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TypeBlog Post...were many excellent applicants but Xin and Fatima stood out for their creativity in the use of fluorescent...shutting off the enzyme. Later exposure to cyan light stopped Dronpa oligomerization and turned the enzymes back...
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Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...first post here. Once you’ve identified some fly stocks and other reagents of interest, the next question...regulated by any chosen promoter, and Bloomington’s Stock Center has fly lines expressing Gal4 under a variety... -
Addgene Depositors Get More Citations
TypeBlog Post... these plasmids. While clearly an outlier, this story had us wondering: is there a larger trend here? ...also get many more citations This isn’t the whole story, though. It would also be great to know how much... -
Science Career Options
TypeBlog Post...are needed in a variety of roles, including: Livestock Breeder Scientific Illustrator Plant Breeder ...hobbies and volunteer experiences, as well as work history can really show that you have some of the abilities... -
Cultivating Community Science at BosLab
TypeBlog Post...sets. Kellen Andrilenas is a Ph.D. grad student at Boston University studying mammalian immunology, and in... get involved with DIY bio If you’re not in the Boston area but are inspired to get involved, check out... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...format. Finally, while we can’t currently produce custom gRNA-containing lentivirus, we are providing lentiviral...packaging plasmids to create your own lentivirus stocks. Our research scientists have provided their standard... -
5 Great Apps for Lab Life
TypeBlog Post...devices to our own interests with the content we store in them: music, emails, chats, pictures, and apps...acids memorized, but the structures are another story. AMINO has been very helpful when I’ve had to explain... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...including synthetic biology. Addgene depositors Christopher Voigt and Jeffrey Tabor have been working on ...given promoter. To harness the power of light, Christopher Voigt’s lab created the first E. coli light-sensitive... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post...Fidelity versions of the endonucleases and/or supply custom buffers as means to avoid this issue. For your...until you have good separation between your bands. Stopping the gel when the bromophenol blue dye line is ... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog Post...calcium events), Greater brightness, Less signal distortion from photoswitching or photobleaching Improved...addgene.org Fluorescent Proteins: Biosensor Plasmids In-Stock AAV Vectors: Biosensors AAV Packaged on Request ... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post... tasksDelegation can feel unnatural for those accustomed to hands-on work. The temptation to "do it yourself...Matteo Tardelli authored the book "Beyond Academia: Stories and Strategies for PhDs Making the Leap to Industry... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...General envelope plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however, may be used across...Element Purpose Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope ... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post...status we call “Archived.” This status allows us to store plasmids and make them available to scientists without...Watanabe R, Taguchi F (2008) Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by... -
How Addgene Creates Content
TypeBlog Post... the blog originally started as a way to easily store and share in-depth answers to frequently asked questions...resources that answer questions our depositors and customers are asking. So how can you get involved? Keep ... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...the frozen cell line becomes non-recoverable, the stock of the mAb becomes limited, which can be a threat...able to isolate RNA from non-recoverable hybridoma stocks, without having to grow up the cells. This RNA ... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...Great! But don’t just stop using it cold turkey. Step back gradually; maybe you stop to check things off... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from the U6 promoter and puromycin resistance...would like to thank all of our depositors and requestors for making their reagents available and for supporting... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog Post...BLACKandSTEM Twitter community. Dr. Page shares her story of how one seemingly innocuous tweet would not only...you advance your research or career? Share your story in the comments below! Additional resources ... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post...box" provided by the manufacturers and enables customized experiments. Projects such as the OpenFlexure...Lachmann, R., Carlstedt, S. et al. A versatile and customizable low-cost 3D-printed open standard for microscopic... -
A Deep Dive into BioBuilder
TypeBlog Post...space offers STEM programming, workshops, and customized programs to meet the needs of students and teachers...blogger, Mary Tamer from BioBuilder! Mary Tamer is a Boston-based writer, editor, and consultant whose work... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...sequence viewer of choice. If the depositor provided a custom annotated GenBank file, this can be found on the...BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using... -
3 Challenges in Plant Synthetic Biology
TypeBlog Post...involved in central anthocyanin metabolism are well understood. Additional work has also identified and characterized...how you were able to overcome them. Share your stories in the comments below! And if you have developed... -
Antibodies 101: Fab Fragments
TypeBlog Post...technique for monoclonal antibodies. J Histochem Cytochem : Off J Histochem Soc 34(6):703–706. https://doi.org... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...refers to the work put into creating a permanent store of knowledge (a schema).” In scicomm, I like to.... It’s a lot easier to read a formulaic fiction story that doesn’t require any detailed retention of information... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...neurons whose function and connectivity are poorly understood,” says Qiu. Qiu developed a dual virus system... light activation and CNO silencing. (D) Immunohistochemistry verification of the dual virus expression... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...directly visualized with fluorescence or immunohistochemistry methods, these proteins are routinely fused... also be identified using antibodies and immunohistochemistry techniques. Thus, these proteins provide... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...2019 Society for Developmental Biology meeting in Boston. The atmosphere was electric! After attending this...(2018): 134-144. PubMed PMID: 29684787. Oviedo, Néstor J., et al. "Long-range neural and gap junction ... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...biomarkers. The lab validated their platform on clinical stool samples by comparison to qPCR and demonstrated that...the SAP-RFP fusion. Then, the cells are washed to stop the production of new particles. With subsequent... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...coordinate emotions, thoughts, and behavior. Historically, scientists study the electrical activity in...interactions in cells because the protein binders can be customized for protein visualization, relocation, degradation... -
The time and cost required to make a plasmid
TypeBlog Post...closing in on the one million plasmids shared milestone in 2018, we wrote a post on the time and cost ...any of the numbers in this post is a mathematical story well beyond my ability to tell. But even from these...