...Newsletter and recently visited Cambridge to spend some time learning and doing research at Novartis. Listen ...scientists Click here to listen! Podcast breakdown (Time : Topic) 0:00 - 7:11 : Introduction - Ghanaian scientist's... opportunities in Ghana. 46:35 - end : Kwabena's time in Boston - Developing microbiome expertise. Many...Many thanks to Kwabena Duedu for taking the time to sit down and talk with us.  Kwabena Duedu, PhD is ...

...plasmid and how much time Addgene has saved researchers. Assuming 5 hours of hands-on time to make each plasmid...others to ensure that everything will ship out in time - from assembling the boxes, to inoculating tubes...scale reagent sharing led us to calculate how much time we have saved researchers. In a recent blog post...

...serotypes may perform better (3). Brain Region  Optimal Serotype Striatum AAV5>AAV9>AAV8>AAV1>AAV6>AAV2...outlined in the table below. Cell Type Cortex Optimal Serotype Oligodendrocytes AAV8 > AAV2 & AAV6 ...quickly. 3. Patience Is Bitter, but Its Fruit Is Sweet Time If you require a quick onset of expression, AAV ...see that gene expression really does not take much time at all. Much of the previous literature using AAVs...3 days vs 7 days vs 14 days etc. To decrease the time until expression, it is possible to use AAV constructs...

...they've managed to keep themselves happy during their time in grad school. As you'll learn, grad school has...topics. Listen to Part 1 Here Listen to Part 2 here! Time : Topic 0:00 - 0:50 : Intro 0:51 - 3:08 : The importance...right that illustrate a grad student's happiness v time throughout graduate school. This is, of course, ...We'd like to thank Niroshi and Ben for taking the time to sit down and talk to us and wish them the best...

...Though, to be fair, I think this is a conservative estimate. As a graduate student, I drank 3-4 cups of coffee...hear that we've been able to help their labs save time and resources by storing and distributing their ...about making such tools open-access. Though it is time for me to refill my large coffee cup and get back... – so if you’re running out of energy, then it’s time for a coffee run! ...

...African countries. Even that grim estimate might prove to be an underestimate. Given all the excitement and...itself that kills you, but the cytokine storm it ultimately sets off.  The virus works its way into immune...Zimmer’s coverage on the subject in the New York Times and on his blog, where he has offered to answer ...

...organisms and their characteristic radiance for some time – there are records describing them dating back ...back to the first century A.D. – the first time anyone truly examined what caused this glowing phenomenon...organisms (E. coli, and later C. elegans) for the first time. After Chalfie’s work, scientists really started...fluorescent proteins with new colors Around the same time, researcher Sergey Lukyanov realized that, although...

...systems, the individual parts are reusable, saving time and money on future assemblies. If you are new to...for eukaryotes! The technology has been used to optimize protein secretion and expression in the biotech...guides, mini screening libraries, direct edit optimization, and more. While there are many CRISPR-Cas libraries...parts can be used in a variety of organisms, saving time and money for labs with diverse applications. The...already in place. MoClo is a fantastic way to save time and money on studies that require many assemblies...

...to as “Epi” illumination. Therefore these are sometimes call Epi-fluorescence microscopes. (See left image...sample. As more than one hole is illuminated at a time, the image can be acquired more quickly. 2-Photon...single focal plane of the sample is excited at one time, limiting photobleaching. Similar to widefield, ..., the entire field of view is excited at one time and captured in a single camera exposure. This is much...technology and parallelization (7) have allowed for real-time monitoring of neuronal activity across substantial...

...importantly, there’s the opportunity cost of your time; an investment of months of your life that may end...and the assay are just as critical for success. Sometimes, you might have a few positive control genes on...way to do something is to do it huge, there are times when a more incremental approach is prudent. What's...      References: 1. Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize...

...use our libraries to do things - sometimes drug screens and sometimes totally different experiments we... others is that, in a relatively short amount of time, cancers become resistant to drugs, particularly...is that if you introduce them into cells one at a time, you could identify those that confer resistance...

...and Nicole Waxmonsky Resource sharing shortens the time needed to go from planning an experiment to performing...using one of these validated gRNAs can save you the time that would be spent making and testing entirely ...CRISPR experiment.  The selection process is often time consuming and there is no guarantee that the selected...demonstrated to work in your system, it is worth spending time sequencing the genomic target to determine whether...

...skill,  time for which most researchers wouldn't have. They are motivated to get data, publish in time, defend... of art to me… The problem is that scientists sometimes forget how to communicate about their work with...its stunning images that is has been featured in Time magazine . Other smaller competitions are now taking...

...Welcome to 2014! Get excited, it’s a new year. A time for resolutions: to wake up and get to the lab earlier...been slower to adapt, likely due to the necessary time and resource investment required to change the status...’ll be able to make updates to protocols in real time. You may also want to consider moving your paper...added or removed samples. Once you've taken some time to get organized, stop back and let us know what...

...account for three years and have only tweeted 6 times: #fail. I’d hoped to use Twitter professionally ...scientists tweet URLs with scientific domains <10% of the time. Figure 3: Top 20 Domains Tweeted by Scientists... contain scientific domains between 0-10% of the time; however, ~20% of life scientists  (biologists, ...ecologists) tweet scientific domains 20% or more of the time (red box). 8. Scientists tend to follow other scientists...

... eight times faster than GRABNE and deactivated three times faster. This enhanced response time allows...generating a measurable signal after electrical stimulation with a single pulse, while nLightR gave a measurable...

... stage, before you order.  Timeline Once your order is placed, we estimate it will take 6-8 weeks for ...and QC assays to be completed and reviewed. The timeline therefore will be as follows:  Request review:...Prep and QC completed: 6–8 weeks Average shipping time: 1–2 days (USA); 3–4 days (international; varies...

...took the time to answer our questions.   Advice for success in academia How can we shape our time in academia...passion. “Love what you are doing!” It helps when the time comes to work with full force. George Church 1....during their studies. They suggest that you make time for extracurricular activities both to develop new...

...with a master’s. At the time my degree felt like a consolation prize, but in time I realized its value. ...helped develop my science communication skills. Ultimately, my degree prepared me to be exactly the type...1. Are you interested in master’s-level jobs? Ultimately a degree is a means to gain employment, so it...

...exceeding a kit’s capacity can actually reduce your ultimate plasmid yield.  Take your average column-based...available for plasmid purification. Kits can be optimized based on the plasmid size, cell model, or overall...size. Some manufacturers offer kits specifically optimized for purification of these large plasmids, while...and for extracting RNA! (That’s a blog for another time, however!) Where can you look for help?  All manufacturers...Different Quantities, Different Uses Plasmids 101: Optimizing Plasmid Yields Bringing Sustainable Practices...

...outstanding material transfer agreement (MTA) turnaround times: less than two days for material requests and less...agreement must be put in place. And subsequently, every time that material is requested, a material transfer ...By default, your TTO must sign an agreement each time a material is being requested or deposited. But,...future requests/deposits. This reduces the processing time of request MTAs and deposit agreements to basically...

...in utero electroporation. Then at your preferred time points, sacrifice the animals and fix the samples...AAVs, it typically takes a few weeks to achieve optimal expression of XFPs. Any longer and the cells may...concentrations or AAV titer for the best results. The timing of sacrifice when using Tetbow AAVs - You will ...Super-resolution mapping of neuronal circuitry with an index-optimized clearing agent." Cell reports14.11 (2016): 2718...

...rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually waiting Work is ... (mostly), but can often achieve more structured time management conducive to work/life scheduling Diverse...to shed light on what paths to pursue. Spend some time really thinking about your motivations and what ...

...an entire classification scheme, known as the Baltimore replication classes, has been developed around...around how they replicate (Figure 2). Figure 2: Baltimore replication classes. Image credit: Louten, 2016...single particle creates many copies of itself, sometimes up to millions of new copies, which are released...And now that you know what viral vectors are, it’s time to dive into how they’re used! We have a growing...

...individuals for green cards because of the cost, long timeline and uncertainty associated with filing a permanent... a series of stages to refine and build skills overtime for success. Many people will have different roles... Ph.D. trained scientist. If the candidate is ultimately looking for a role in a different functional ...must be named in the media report (NPR, New York Times, respected online sources) and the media should ...must show that your company employs at least 3 full-time people engaged in researcher activities. The EB-...

...repository, they remain accessible during these times. Centralized reagent repositories, also known as...access to validated reagents, especially during a time when these materials may not be available from their...Addgene and has already been requested dozens of times in 2 weeks. This is a striking example of the contributions...Microbiology 14:299–303 . https://doi.org/10.1016/j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol...

...have been able to study the effects of various stimuli on global translation, but a visual technique to...by tracking the translation of a single mRNA over time. Both are helping researchers explore the complexity...that translates the mRNA. To permit tracking over time, Yan et al. devised a system that labels both mRNAs...fused to the SunTag-specific antibody scFv. Each time the reporter is translated, the SunTag sites on ...

...Use the Viral Service Tips for 1st Time CRIPSR User (By a 1st Time CRISPR User) Tips for Titering Your...from 7.4 to 6.8). It’s possible that a longer vent time would be sufficient to return the sample back to...handling recommendations Not all O-rings function optimally at -80°C. For samples that are shipped on dry ...

...visit   Design and communicate a research plan Your time at the new research site is limited, and we all ... know that science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for...research and teach you new techniques. Show up on time (don’t make your bench mentor wait on you!), make...amazing career opportunity, but also a really good time! If it sounds right for you, start thinking outside...

...a high titer Cas9 lentivirus prep takes a bit of time and effort that, for many groups, may not be worthwhile...enough lentivirus for you to both perform an optimization-scale infection and at least one screening-scale... robust. What you don't need to do is spend your time troubleshooting reagent production, because that's...make new lentiviral preps on your own, fear not - anytime you order ready-to-use lentivirus from Addgene,...