We narrowed to 795 results for: Tec
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TypeBlog Post...States while caring for a patient, despite wearing protective gear, and now comes news of a second Texas healthcare...essential in allowing those infected cells to go undetected by the immune system in a novel way, by shielding...
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Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog Post...grade DNA Molecular cloning includes a set of techniques that are used to insert recombinant DNA from .... As a consequence, the preparations for this technique must be larger scale, to allow the extraction ... -
A Conference By Postdocs For Postdocs: Future of Research
TypeBlog Post...Group. University of Pennsylvania has the Penn Biotech Group and PBG Healthcare Consulting. I know of ...that have completely student/postdoc-driven biotechnology clubs such as Penn State, Berkeley and Harvard... -
Keeping Up With CRISPR/Cas9
TypeBlog Post... understanding and application of CRISPR/Cas9 technology are coming along at a fast and furious pace. ...Way to Edit DNA" and "This Protein Could Change Biotech Forever." David S. Weiss of Emory University summed... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog Post...molecules are very useful tools because we can easily detect and measure the light they emit. Proteins that ...closer look at how they’re used, see some new techniques that take advantage of them, and give some tips... -
Our Most Popular Blog Posts from 2019
TypeBlog Post...Jennifer Tsang Prime editing is a new CRISPR technique from David Liu’s lab that gives more control and...into bacteria or eukaryotic cells is a common technique in molecular biology and scientific research. ... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog Post... Genome-Wide Screening Browse 28 Hot Plasmid Technologies from 2015 Additional Resources on Addgene.org... Hot Plasmid Pages to See the Latest Plasmid Technologies ... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog Post...assay, sequencing, RT-qPCR, or immunoblotting to detect. Another approach is to target two exonic sites...design, cloning, and delivery, as well as their techniques for creating deletions, identifying these deletions... -
What's New in CRISPR - March 2020
TypeBlog Post...They generated these editors using the BE4max architecture. The new editors reported in this study have...relative to BE4max. Read the paper in Nature Biotechnology Find the base editor plasmids CRISPRi in Caulobacter... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine. Neuron, 102(4), 745-761.e8....Fluorescent Biosensors Which Fluorescent Microscopy Technique is Best for Me? In Living Color: The Skinny on... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog Post... curator of the thousands of amazing plasmid technologies contributed by our depositors, Addgene strives...certainly not least, we often feature new plasmid technologies on our blog and encourage you to fill out this... -
London Calling - Addgene’s New Europe Office in London
TypeBlog Post...Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids to study the expression of an unknown...plasmid sharing? Check out our Top 10 Plasmid Technologies blog post Or look at our "10 Years of Plasmid... -
9 Tips to Achieve Success in Academia
TypeBlog Post...theory or practice; business-like or artistic; tech, anti-tech or pragmatic.” Advice for pursuing careers... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post...reported doing in a paper that appeared in Nature Biotechnology back in 2011 and then in another Nature Methods...protein for in vivo imaging. Filonov et al (Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918.) Near-infrared... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post...Chemogentic and optogenetic technologies have pushed the boundaries in neuroscience by granting targeted...activity. While they serve similar purposes, both techniques offer researchers different advantages and limitations... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...overcomes all of these labor-intensive and invasive techniques to reliably label any post-mitotic cells in Drosophila...et al., 2019. Applications for SPARC SPARC technology is versatile and can be applied across cell types... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog Post...services we provide like quality control, curation, technical service and open-access educational resources....nonprofit? One big reason is that scientists and technology transfer offices trust us to work for them with... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog Post...intertwined. In this blog we will review how CRISPR technology has brought drug and genetic screens together...of control have also been added to the CRISPR technology to allow time-controlled edits to occur, such... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog Post...also do videos demonstrating an experiment or a technique to students, like how to pour a gel [for] electrophoresis...really cool to take a step back and look at new technologies in education and ask ‘Can we implement that?... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog Post...interview three science communicators who work in the biotech, education, or nonprofit industries. First up ... a job as a Science Writer at Integrated DNA Technologies (IDT). “It was fairly accidental. I was finishing... -
Cellosaurus: A Cell Line Information Database
TypeBlog Post...graduate student down the hall who got it from some technician downstairs who got it from who knows where), ...Cellosaurus, a Cell-Line Knowledge Resource. J Biomol Tech. 2018; 29(2): 25-38.... -
Tips for Improving Your Next Manuscript
TypeBlog Post...sections. The focus groups allowed us to try out techniques that were discussed in the larger sessions and... Well what does that mean? If you start a new technique, then type up the materials and methods. Over ... -
Networking and Inspiring Young Scientists at Freshers Fairs
TypeBlog Post...learn about Synthetic Biology and exciting new technologies in the field. One of the main aims of the society...networking to promote the creation of start ups and biotech spin offs. The Synthetic Biology Society also aims... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog Post...field. Advances in genome engineering & CRISPR technology That afternoon's workshop, entitled "Advances...amazement at how much has been done with CRISPR technology in just 2 years and excitement about how much... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog Post...postdoc association, started a speaker series or biotech club, managed their own blog or podcast, initiated...the board of a professional society, solved a technical problem, had an alter ego as a mad scientist for... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog Post...scientists studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure that ...applied for the government sponsored Paycheck Protection Program loan and received enough funds to make... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post...bacterial strains, such as Stbl3s. ITR loss can be detected by restriction digest using enzymes, like SmaI...Kamens J, Fan M (2019) Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...containing lentiviral vectors the iBARs can be detected by next generation sequencing. In order to avoid...measurements, or monitoring in vivo. To overcome these technical issues, Sabrina Büttner’s lab designed a calcineurin... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...reliably confirm the identity of AAV genomes and detect contaminating AAV DNA. In addition, we wrote an...minutes and the scripts can be easily customized to detect other sequences of interest. While the VGS process... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog Post...Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative, will allow Addgene to... The development of monoclonal antibody (mAb) technology circumvented many of these issues by creating... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post... genetically encoded antibody based probe that detects the widely used HA epitope in diverse cellular ...dynamics in vivo. In the future, this stitching technique could be applied to other epitopes and scaffolds... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...desired identity. The development of single cell technologies, such as single cell RNA-seq (scRNA-seq), now...multi-generational cell lineage trees. CellTags were detected over a 10-week period. Their expression decreased... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...Their aim - to red-shift the boundaries of FP technology by shifting the emission maxima toward the red...However, a challenge in optimizing base editing technologies across several cell types is the lack of a rapid... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post...The sky is really the limit for this emerging technology! Resources and References References Orguc, ...modulation of gut and brain neural circuits. Nature Biotechnology, 1–13. https://doi.org/10.1038/s41587-023-01833... -
Antibodies 101: Chimeric Antibodies
TypeBlog Post...secondary to detect Protein A and green fluorophore-tagged anti-mouse IgG2a secondary to detect Protein B... -
The Strength of Story Telling
TypeBlog Post...STAR (Situation, Task, Action, Result) interview technique, which has been addressed in much better pieces...story should be too. Use drama Use any rhetorical technique you have in your arsenal to make sure people pay... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog Post... to detect mutants, but this method limits screening to a few hundred worms, making the detection of rare... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog Post...especially for life science researchers. Many biotechnology companies are looking for PhD or Master’s level...capabilities, courses and office skills. I removed my techniques and protocols, courses taken during my PhD and... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog Post...translational approaches for scientific tool and technique development. However, careers outside of the lab...Management Leadership Networking University technology transfer office positions Market analyses... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post...materials available rapidly to scientists (Nature Biotechnology 2020). The continued success of repositories...j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020...