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  2. CRISPR Guide Update

    Type
    Blog Post
    ...the Cascade-Cas3 system. We also describe newly engineered Cas9s with more flexible requirements and enhanced...

  3. Retrovirus Guide

    Type
    Guide
    ...Questions (FAQ) about γ-Retroviral Plasmids How do engineered γ-retroviruses differ from lentiviruses? There...There are a few important differences between engineered γ-retroviruses and lentiviruses. First, these viruses...

  5. The 12 Days of CRISPR

    Type
    Blog Post
    ...the 1st day of CRISPR, Addgene gave to me: An engineered prime editor with enhanced editing efficiency...

  9. Summer Fun at Addgene!

    Type
    Blog Post
    ...webinar “Navigating an Expanding Landscape for Engineered Systemic AAVs” with the director of CalTech’s...

  10. Our New Antibody Mascot is....

    Type
    Blog Post
    ...  This as-yet–unnamed friend is a huge fan of scavenger hunts. In fact, they’ve been following a set of...

  11. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...2xNLS-tdTomato-WPRE-hGHpolyA AAV9, PHP.eB Controls Feng New viral service, multiple serotypes Looking...

  12. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...dendritic spines!  Curious? Watch our AAV Data Hub Challenge recording to see two reports presented by the ...

  14. Editor's Choice, October 2016

    Type
    Blog Post
    ...Grad School Advice Part 2: Building Community Engineering the Plant Genome Using CRISPR/Cas9 Healthcare...

  17. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...experiments. Speaking of CRISPRs, we were excited to have Feng Zhang (of the Broad Institute of MIT and Harvard...

  18. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...papers freely available here. -PLoS and The New England Journal of Medicine have similarly curated their...

  19. Plan Your Experiment

    Type
    Guide
    ...gRNAs that have been used successfully in genome engineering experiments. Using validated gRNAs can save your...Publications Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9 system. Methods in Molecular...

  20. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...deamination of RNA cytosines in human cells. Thus, they engineered two CBE variants with mutations in the rat APOBEC1...

  25. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ... Post on CRISPR Methods for Bacterial Genome Engineering Learn about Science Communication using Twitter...

  28. Reflections and Looking Ahead

    Type
    Blog Post
    ...closer to making an impact on humanity's greatest challenges. Thank you for being part of this incredible ...

  29. Editor's Choice, August 2016

    Type
    Blog Post
    ...its kind for the Addgene blog - Addgene Software Engineer Kris Shamloo wrote a very interesting post about...

  32. Lentiviral Guide

    Type
    Guide
    ...use the HIV protein tat in order to produce full length virus from the transfer vector during the viral...This deletion abolishes transcription of the full-length virus after it has incorporated into a host cell...

  41. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ... pAAV-EF1a-fDIO-Cre AAV5 Recombinases Esteban Engel New serotype pAAV-hSyn-Cre-P2A-dTomato AAV PHP.eB...

  47. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... other Cas proteins as well, both natural and engineered. What these proteins all have in common is the...
Showing: 601 - 650 of 800 results