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We narrowed to 911 results for: Tro;

Showing: 601 - 650 of 911 results
  1. Single-cell tracking of lineage and identity with CellTag

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    Blog Post
    ...cell types. MEFs were reprogrammed into iEPs by retroviral expression of transcription factors Foxa1 and.... Adding Mettl7a1 to the standard Foxa1-Hnf4α retroviral overexpression cocktail yielded a three-fold ...
  2. Advice for Moving Into Sales After Your Science Postdoc

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    Blog Post
    ...build, sales and marketing gives you a great introduction to the financial world. It teaches you about...you can find some great information on ELISA troubleshooting, ELISA kits and cell death assays. You can ...
  3. "Build Your Plasmid": The Game - Play to Teach

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    Blog Post
    ...YOUR PLASMID has been developed to teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies...at FRESCI particularly interested in advanced in vitro models technology. Follow him on twitter @FRE_SCI...
  4. Scientists Map the SARS-CoV-2-Human Interaction Network

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    Blog Post
    ...Nsp5. Our set also includes a GFP construct as a control. For easier portability, all viral genes are flanked...clone selection. Expression is driven by a fairly strong EF1alpha promoter. We have verified expression ...
  5. 25 Work From Home Tips for Scientists

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    Blog Post
    ... one figure. If you aren’t there yet, write introductions to potential publications or prepare a review...isn’t for a while. Getting started early on introductions and literature reviews could make the months...
  6. Networking Follow-up: I Met a Person. Now What?

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    Blog Post
    ...person or virtually) will make the relationship stronger.  If appropriate, invite them to speak for your...chocolate-covered strawberries for helping with an introduction that helped someone get a job interview. Even...
  7. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    Blog Post
    ...complex genetic diseases, Alzhiemer’s disease (AD).  Troy Rohn and his lab are researching a way to treat ...proteins aid the limitation of these mutations, to control the CRISPR system activity.  Using an AAV vector...
  8. Cultivating Community Science at BosLab

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    Blog Post
    ... the lab, so in December 2015 we held our first intro to bacterial transformation course (read our blog...bacterial transformation guru and instructor for our intro to biohacking courses. Our members pursue a diverse...
  9. Pushing the Limits of DNA Assembly

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    Blog Post
    ...necessarily have to be. Regions of homology can be introduced to fragments via PCR using primers that contain...patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. Many thanks to ...
  10. Resume Writing for Non-academic Science Careers

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    Blog Post
    ...you did. Emphasize the skills. If you studied astronomy or bioinformatics data, then the chance is that...Communications Coordinator for the Department of Physics & Astronomy, University of British Columbia. Originally on...
  11. Advice for Scientists Starting a Lab

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    Blog Post
    ...equipment always helps. But how does one build a strong collaborative research team motivated by similar...selectively picking the first couple of hires to build a strong, collaborative team. And take advantage of the ...
  12. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post
    ...Please keep in mind that this is just a brief introduction to the wide, wild, and fascinating field of ...Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies Resources on Addgene.org ...
  13. Communicating Your Science With Help From ComSciCon

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    Blog Post
    ...already grown out of our workshops. If you're having trouble finding science communication opportunities in ...of its Leadership Team.  Nathan did his PhD in astronomy and works as a data scientist in the entertainment...
  14. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    Blog Post
    ... Image from Wen et al., 2017. Once you’ve introduced the reporter into the cells, you’re ready to ...sequence is also found in the reporter gene). A control RFP expression plasmid.  With this set up, all...
  15. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    Blog Post
    ..., you’ll want the proper positive and negative controls for your PCR.  Find the details and learn more... this blog post for tips for analyzing and troubleshooting Sanger sequencing results. At Addgene, we now...
  16. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    ...base pairing between these ~4 base overhangs isn’t strong enough to hold a plasmid together through transformation... reading Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Li MZ, Elledge...
  17. A Deep Dive into BioBuilder

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    Blog Post
    ...extracts to perform transcription and translation in vitro, and the new BioBuilder module allows students to...Assembly Standards Find Plasmids for Creating and Controlling Gene Networks Browse All SynBio Plasmids ...
  18. Screening for Successful Genome Editing with Digital PCR

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    Blog Post
    ...minimally informative. In this blog post, we’ll introduce digital PCR as an emerging validation technology...assays for mutation screening Now that we have introduced how digital PCR works, let’s explain what kind...
  19. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post
    ...team develops methods, performs extensive quality control, generates  educational materials, and provides...details about virus production protocols and quality control can be found on Addgene’s Viral Production webpage...
  20. Plasmids 101: Blue-white Screening

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    Blog Post
    ...operon.   Tips for blue-white screening Use a good control: Transform the backbone plasmid without insert....reproduce to form colonies (used to complement auxotrophic cell lines), or genes which rescue a lethal phenotype...
  21. 3 Challenges in Plant Synthetic Biology

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    Blog Post
    ...other than to sustain those techniques. We feel strongly that the scientific and the agricultural community...aesthetics with this technology and giving consumers control over their interaction with it, we hope to inspire...
  22. What's New in CRISPR - November 2021

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    ...for cellular delivery of RNAs By Mike Lacy The retroviral-like protein PEG10 specifically binds its own...small insertions to allow in-frame deletions and introduce epitope tags and RNA transcription start sites...
  23. RNA Extraction Without A Kit

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    ...degraded. To effectively isolate intact RNA, a fast, strong protein denaturant would be required- something...late 1970’s, Chirgwin and colleagues showed that a strong protein denaturant, guanidinium thiocyanate, did...
  24. Click Biology: How to Click and Play to Explore Biology

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    Blog Post
    ...triggering new cellular behavior like degradation or electrophile signaling. Many of these tools can be found ...References Devaraj, N. K., & Finn, M. G. (2021). Introduction: Click Chemistry. Chemical Reviews, 121(12),...
  25. To Each HIS Own

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    ...the lab (for a refresher see Antibodies 101: Introduction to Antibodies.) Unfortunately, for some proteins...Protein Tags Antibodies 101: ChIP Antibodies 101: Introduction to Antibodies References Hochuli E, Bannwarth...
  26. Antibodies 101: Affinity Reagents

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    ...of the two fragments. However, rigidity can be introduced through mutation, allowing diabodies to be used...FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction to Antibodies Additional resources on addgene.org...
  27. Celebrating 15 Years of Scientific Sharing

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    ...the hands of our finance team, now 3.5 Addgenies strong. The Addgene Board of Directors is mature and...and I believe they know and trust this. We’ve introduced cutting edge practices and benefits and these...
  28. Antibodies 101: Fab Fragments

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    ... means that F(ab’)2 fragments may have overall stronger interaction with their antigens than a Fab or ....2022.100301  Additional resources on the Addgene blog: Introduction to AntibodiesSingle Chain Fragment Variables...
  29. CRISPRainbow and Genome Visualization

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    ...successfully labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the method is specific...of the Pitfalls When Using Fluorescent Proteins Control Protein Activity with GFP Resources on Asddgene.org...
  30. The Future of Research Symposium Boston 2015

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    ...Department at University of Maryland College Park, is a strong supporter of increasing the number of postdoctoral...postdoc-related data collection and analysis. He is also a strong supporter of science Master’s programs. This will...
  31. Career Coaching for Scientists: Why and Where Do I Find One?

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    ...their goals.  Check out Joanne's Reddit AMA I am a strong advocate for peer mentoring, but volunteer mentors...contact is Twitter’s @DrQualls. Marquita Qualls of Entropia Consulting works remotely, will travel for speaking...
  32. 5 Steps to Writing Grant Proposals for Grad Students

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    ...institution. Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to...will convince the reviewers that the proposal is strong, well-thought out, and will be successful when ...
  33. Lab Automation at Addgene

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    ...into the robotics spirit include: Start using electronic multichannel and single channel pipettes. These...by one. It seems very simplistic and it isn’t electronic but it is one of the most helpful machines I ...
  34. Progress Towards a PAM-Free CRISPR

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    Blog Post
    ...want to knock out a gene, a frameshift mutation introduced anywhere within the early coding region will ...  Susanna Stroik...
Showing: 601 - 650 of 911 results