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We narrowed to 1,092 results for: addgene

Showing: 601 - 650 of 1092 results

  1. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ... wild type and mutant R bodies can be found at Addgene here. What can R bodies do for you? R bodies are...26814170. Find Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., ...Central PMCID: PMC26518. Additional Resources on the Addgene Blog Read About Other Hot Plasmid Technologies...Plasmid Tools for the Repository Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids...

  3. Science Communication for Everyone

    Type
    Blog Post
    ...science communication Resources on Addgene.org Learn how Addgene engages with the community ...BioTechniques.     Additional resources on the Addgene blog Find all of our science communication posts...

  4. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...will make those new plasmids available through Addgene, and they hope others will do the same. “This isn...10.1186/1754-1611-8-7. PubMed.  Related Resources Addgene's Synthetic Biology Overview More Synbio Blog Posts...

  5. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...The complete GreenGate toolkit, now available at Addgene, also gives the option to stack several expression...motivations for making GreenGate available through Addgene,” Lohmann said. “We strongly believe the free sharing...

  6. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...line), armed only with viruses obtained through Addgene’s viral service and the methods sections of scientific...of performing and analyzing a gene edit using Addgene’s lentiviral CRISPR tools. Schematic overview...repeatability and bad news for a screen. Because Addgene viruses are provided at a specific titer, you can...Protocols 2007; 2(11): 2734-46. PubMed PMID: 18007609. Addgene Materials Used 1. lentiCas9-Blast (Plasmid #52962...PMCID: PMC4744125. Additional Resources on the Addgene Blog Genome-wide Screening Using CRISPR/Cas9 Deliver...Mouse Models Using CRISPR/Cas9 Resources at Addgene.org Generating Stable Cell Lines with Lentivirus...

  7. Antibodies 101: Conjugation

    Type
    Blog Post
    ... Resources on Addgene.org Unconjugated antibodies at AddgeneAntibody plasmids at Addgene... functionality of the conjugate. For example, Addgene’s antibodies are provided in a buffer with the antimicrobial...10.1038/nmeth.4380  Additional resources on the Addgene blog Antibodies 101: Buffers, Storage, and ConjugatesAntibodies...

  8. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...10.1186/s12870-020-02388-2 Resources on Addgene.org Addgene’s Modular Cloning Guide EcoFlex MoClo Kit...with characterized behaviors are available from Addgene and other sources; second, characterizing a part...MoClo Kit MoClo Plant Parts Kit Resources on the Addgene blog High Complexity Golden Gate Assembly with...

  9. To Each HIS Own

    Type
    Blog Post
    ...  Resources on addgene.org Addgene Antibody Guide Ready-to-use antibodies from Addgene More Resources ...Resources on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies 101: Introduction to ...

  10. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...are many more useful viral vectors available at Addgene: Follow our viral vector blog posts for updates... Get the ready-to-use lentiviral prep from the Addgene viral service. CRISPR technology lentiCRISPR v2...PMCID: PMC4830361. Additional Resources on the Addgene blog Viral vector topic page Beginners guide to...guide to all things lentivirus Resources on Addgene.org Gamma-retrovirus Guide Lentivirus Guide ...

  13. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...review of Cpf1, check out this blog post or see Addgene's CRISPR guide page for a review of Cas9.  For a... the Cpf1 multiplexing plasmids available from Addgene. If you have any questions or thoughts about Cpf1...Central PMCID: 4638220. Additional Resources on the Addgene Blog Cpf1: A New Tool for CRISPR Genome Editing...in Heaven: CRISPR/Cas9 and AAV Resources on Addgene.org Browse Our CRISPR Multiplexing Resources Find...

  14. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...fluorescent biosensor that was recently deposited with Addgene by Dr. Mark Prescott. This system was developed...mitochondria-targeted variants are available from Addgene. References 1. Rosado, C., Mijaljica, D., Hatzinisiriou...PubMed PMID: 19185711. Additional Resources on the Addgene Blog Beware of Fluorescent Protein Oligomerization...Fluorescence Microscopy Additional Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent...

  15. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...with GFP. These plasmids are available through Addgene, and the stem cell lines we’ve made using them ...a variety of endogenous gene tagging systems. Addgene’s CRISPR-based Protein Tagging collection includes...bioRxiv (2017): 123042. Additional Resources on the Addgene Blog Learn More about Homology Directed Repair... Our Fluorescent Protein Posts Resources on Addgene.org Browse CRISPR Tagging Plasmids Browse the Fluorescent...

  16. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...Science Careers Resources on Addgene.org Find Career Opportunities at Addgene  ...leading up to this date, as discussed in a previous Addgene blogpost and more recently in a paper published...Research @FORsymp.   Additional Resources on the Addgene Blog Read Gary's Previous Article: Changing Labor...

  17. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...Please check out Addgene’s Choosing a Molecular Cloning Technique page and the Addgene Blog for a variety...PubMed Central PMCID: PMC209908 Resources on the Addgene Blog Plasmids 101: Common Lab E. coli Strains ...all of our Plasmid Cloning Posts Resources on Addgene.org Choose a Molecular Cloning Technique Find Plasmid...

  18. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...Resources on Addgene.org: Sign up to receive Addgene’s Science Career Guide Check out Addgene’s job openings...but then relax.  Additional resources on the Addgene blog: Tips for writing a good cover letter 6 Steps...

  19. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...I love Addgene's new Antibody Guide and not just because I helped create it. But while antibodies, as...space to give proper credit to. Thankfully, as Addgene's resident blog editor, I could take the needed ...References and Resources Additional resources on the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies...resources on addgene.org  Antibody GuideRecombinant antibodies available from AddgeneBrowse our recombinant...

  20. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by both highlighting...currently work on the Scientific Support Team here at Addgene, so troubleshooting molecular biology experiments...choose.    Resources Additional resources on the Addgene blog More from the transferable skills guide: ...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ...

  21. Engaging with science and society at pgEd

    Type
    Blog Post
    ... Resources on Addgene.org Learn how Addgene engages with the community on our Addgene gives back page...biology and society.     Additional resources on the Addgene blog Check out our series on careers in science...

  23. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...suggest some coaches? Yes I can. Please note that Addgene does not endorse any of these coaches and I can't...investment in your future. Additional Resources on the Addgene Blog 6 Steps to Submitting a Resume That Gets ...Future of Research Symposium Resources on the Addgene Website Check out Our Career Page for Recent Job...

  24. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...sequence. (A) Plasmid sequence from pLVUHshp53 (Addgene plasmid #11653). Full shRNA sequence is highlighted...boxes. (B) Plasmid sequence from pcDNA3-miR21 (Addgene plasmid #21114). The miRNA sequence is highlighted...button_text" is_json="true" %}{% raw %}"Browse Addgene's shRNA plasmid collection"{% endraw %}{% end_module_attribute...s12551-021-00902-w Additional resources on the Addgene blog RNA Interference in Plant Biology: New Tools...https://www.addgene.org/collections/mammalianrnai/","href_with_scheme":"https://www.addgene.org/collections... Plasmids 101 Posts Additional resources on addgene.org Mammalian shRNA Tools for RNAi Molecular Biology...

  25. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...vectors (for a more detailed explanation, check out Addgene's Lentiviral Guide). To produce lentiviral vectors...integrase-deficient packaging plasmid available at Addgene: psPAX2-D64V. This construct has the D64V mutation... how to package lentiviral vectors, check out Addgene’s Lentiviral Guide and our lentiviral production...Central PMCID: PMC9377332 More resources on the Addgene blog Viral Vectors 101: The Retroviral Life Cycle...every conversation! This blog post was written by Addgenie Rebecca Hutcheson.   References and resources... to All Things Lentivirus More resources on addgene.org Lentiviral Guide   Popular lentiviral vectors...

  26. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...ability to use rats as disease models.  Check out Addgene’s collection of plasmids containing Rattus norvegicus...disease alleles during and after embryogenesis. Addgene has over 600 plasmids that contain Xenopus genes...popular model organisms in the lab, check out Addgene’s plasmid collections to help start your experiments...E-coli-as-a-Model-Organism.aspx. Additional resources on the Addgene blog: Read more about model organisms in the first... in our CRISPR 101 blog series Resources on Addgene.org: Browse our plasmid collection page Find more... Various Addgenies...

  28. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...PubMed Central PMCID: PMC3969860. Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using... Plasmids to Mammalian Cells Resources on the Addgene Website Visit our CRISPR Guide Pages Browse Our...

  29. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...vectors to generate a kit which is now available at Addgene. “We called it cloning by mail,” Wood says, and...PMCID: PMC4353587. Additional Resources on the Addgene Blog Learn How the Clon Tracer Library Has Been...growth, survival, and differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs/ Finding...Technologies from 2015 Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled...

  30. Top 10 Open Science Developments of 2013

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    Blog Post
    ...should come as no surprise that those of us at Addgene believe in the power of sharing, and especially...And, last but not least (certainly not for us), Addgene launched this blog, A Better Way to Share Science...

  31. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ... which is similar to PR-T4P, is available from Addgene. If you use these published protocols and want ...genes, we encourage you to deposit them back with Addgene and expand the number of genes that other classrooms... PMCID: PMC304099. Additional Resources on the Addgene Blog Arabidopsis in Education Learning Synthetic...Models for learning about CRISPR Resources at Addgene.org Find plasmids from the Sánchez lab Find plasmids...

  32. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...vectors Learn about Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use AAV...PubMed PMID: 25665714. Additional Resources on the Addgene Blog Check out our Viral Vectors Featured Topic... AAV in Addgene's Viral Service Check out our AAV Production Protocol Check out our AAV Purification Protocol...

  33. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...in large sections of DNA can be more difficult. Addgene depositors have developed new CRISPR-based methods...please consider sharing them by depositing with Addgene! Figures were adapted from: Hall B, A Limaye, ... PMCID: PMC2782548 Additional Resources on the Addgene Blog Get the Basics of Homology Directed Repair... Used to Turn Genes On and Off Resources on Addgene.org Find Mammalian Expression Vectors Find Cre-Lox...

  34. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...skill set Resources on the Addgene website Become of a member of the Addgene depositor community ...your scientific conference? Resources on the Addgene blog Read other posts on scientific sharing, scientific...

  35. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...prokaryotic terminators and deposited several with Addgene (1). Prokaryotic polyadenylation Although mostly...General overview BGH rbGlob SV40 Resources on the Addgene Blog Learn how the Promoter Controls Gene Expression...Field? Check out Our Plasmid Collections! Join the Addgene Community      ... all of our Plasmids 101 Posts Resources on Addgene.org Browse our Plasmid Reference Pages Just Starting...

  36. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...published a protocol that would work for your system. Addgene has depositor submitted protocols and links to ...of CRISPR delivery. There are a few plasmids at Addgene that have been published as CRISPR testing tools...writing of this post. Additional Resources on the Addgene Blog Check out our CRISPR Featured Topic Page ...User (by a 1st Time CRISPR User) Resources on Addgene.org Browse CRISPR Plasmids for Mammalian Systems...

  37. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ... several hundred plasmids from this study with Addgene. Read on to learn more about their work, and how...PMCID: PMC4737758. Additional resources on the Addgene blog Learn about our cancer collection pages Read... Read all cancer blog posts Resources on Addgene.org Find plasmids for your research Find cancer research...

  38. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...Immunofluorescence Resources on Addgene.org Find recombinant antibodies available at Addgene Protocols for Immunocytochemistry...IHC validated or ICC validated antibodies from Addgene), or be prepared to run some extra controls and...three targets or you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed...10.1073/pnas.2018488117 Additional Resources on the Addgene blog Introduction to Antibodies Which Fluorescence...

  39. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ... 93–103 Additional resources on the Addgene blog Browse Addgene's viral vectors articles Find tips and...working with viral vectors Resources on Addgene.org Check out Addgene's written and video protocols ...contamination periodically. For more tips, check out Addgene's video Getting Started with Tissue Culture below...environmental contaminants.  Figure 2: An Addgenie works in the tissue culture hood while wearing...

  40. Antibodies 101: Validation

    Type
    Blog Post
    ... post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ready-to-use...next time you test an antibody (perhaps one from Addgene!), consider sharing your results so that others...References and Resources Additional resources on the Addgene blog:  Still trying to choose an antibody? Check...-to-use antibodies from Addgene Designing a recombinant validation experiment? Find plasmids here!  References...

  41. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...an instructional text file. What's available at Addgene? In total, we constructed and tested eight two-...in the table below and can be requested through Addgene. Their plasmid maps and GenBank files can be found...the plasmid maps and GenBank files uploaded to Addgene. We also recommend inserting self-cleaving hammerhead...PMCID: PMC4245323. Additional Resources on the Addgene Blog Learn How Piggy Bac Vectors Can Be Used for...of Our SynBio Posts Additional Resources on Addgene.org Visit Our SynBio Pages Check Out More Lu Lab...

  44. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...that expound on bench skills and experience. Most Addgene PhD level job descriptions clearly describe that....  You might also enjoy the other posts in the Addgene Blog Scientist Careers Series.    ...

  45. New Antibody Animation!

    Type
    Blog Post
    ...isotypes found in mammals.      Subscribe to Addgene's YouTube channel for more helpful animations, useful...

  46. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...own mutant flies. Find Drosophila Resources at Addgene The birds and the bees when it comes to fruit ...basic knowledge to start working with flies. Like Addgene, the Drosophila community is highly sharing-oriented... a “pound symbol”. Additional Resources on the Addgene Blog Methods for Site Specific Recombination in...Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila Plamids Drosophila CRISPR Resources...

  48. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...Antibodies Resources on Addgene.org Find recombinant antibodies at Addgene Find plasmids encoding recombinant...the-process-of-fixation-and-the-nature-of-fixatives/  Additional resources on the Addgene blog: Antibodies 101: Introduction to Immunofluorescence...recombinant antibodies at Addgene  ...

  49. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...interest; more recently we have also been using Addgene CRISPR vectors to generate cell lines with knockouts...figure 1.   Find Additional Useful Protocols on Addgene's Protocol Pages Agarose cloning method procedure...som.umaryland.edu.   Additional Resources on the Addgene Blog Tips for a 1st Time CRISPR User (by a 1st...Plasmids 101: Mammalian Vectors Resources on Addgene.org Check Out Our Protocols Pages Browse Fluorescent...
Showing: 601 - 650 of 1092 results