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1. Course-Based Undergraduate Research Experiences (CUREs)

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   ...References and Resources References Bangera, G., Brownell, S. 2014. Course-based undergraduate research experiences...Sciences Education. 15(4):ar55. Ma, Y., Mukherjee, S., Uzzi, B. 2020. Mentorship and protégé success in...

2. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...Vanderperre S, Paul R, Björklund M, Ahr D, Ahmed AW, Spinelli L, Brun C, Basler K, Merabet S (2018) Generation...

3. CRISPR 101: RNA Editing with Cas13

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   ...identified that a S375A mutation in RESCUE (named RESCUE-S) offered ~76% on-target C-to-U editing while reducing...from this paper at Addgene. Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a...

4. Droplet Digital PCR for AAV Quantitation

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   ...recommended. Decrease your ramp rate. A rate of 2C/s is recommended to ensure an even temperature change... all of the droplets, but you can go as low as 1C/s. Increasing the elongation time to 2 minutes and the...

5. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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   ... the genome to introduce the genetic modification(s) contained within it by selecting for Zeocin resistance...19416369. Kulakauskas, Saulius, Arvydas Lubys, and S. Dusko Ehrlich. "DNA restriction-modification systems...

6. Tips for arabidopsis transformation

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   ...transgenic plants in the process.     References 1. Emami, S., Yee, M. and Dinneny, J. R. (2013) ‘A robust family...24032037. PubMed Central PMCID: PMC3759027. 2 Harrison, S. J. et al. (2006) ‘A rapid and robust method of identifying...

7. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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   ...Zebrafish (Danio rerio) have been used since the 1930’s in a range of biological studies, including investigations...data in useful ways. Zebrafish mutants In the 1990’s, two revolutionary large-scale random mutagenesis ...

8. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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   ... adult mouse hematopoietic stem cells. Its ligand(s) has not been identified. While LY6A or other Ly6 ... these two references: Dayton, Robert D., Mychal S. Grames, and Ronald L. Klein. "More expansive gene...

9. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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   ... Jul 27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an exceptional genome... this paper at Addgene.  3. Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a...

10. Lentiviral Vector Uses and Overview

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    ... Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer A, Cornetta K, Galacteros...Beuzard Y, Gluckman E, Bushman F, Hacein-Bey-Abina S, Leboulch P. Nature. 2010 Sep 16;467(7313):318-22....

11. A Career in Grants: How To Become a Grant Professional

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    Blog Post

    ...transferable. Use your experience and your passion(s) to guide you in building a grant career. Do you have... client or job will save you and the organization(s) you serve time and resources. 3. Get a mentor, find...

12. Light Sheet Fluorescence Microscopy

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    ... menschlichen und tierischen Praparaten. Leipzig: S. Hierzel.
 J.A Dent, A.G. Polson, M.W. Klymkowsky....1983–1995. Pubmed. K. Chung, J. Wallace, S.Y. Kim, S. Kalyanasundaram, A.S. Andalman, T.J. Davidson et ...

13. Plasmids 101: Protein tags

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    ... peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be fused to recombinant...Purification Very small size, rarely affects function S-tag KETAAAKFERQHMDS  1.8 Solubility and Affinity Abundance...

14. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

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    ...CRISPR/Cas9 vector system. Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T. Sci Rep. 2014 Jun 23;4:5400...

15. Plasmids 101: Modular Cloning Applications and Kits

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    ... then the MoClo YTK Kit specifically designed for S. cerevisiae is at your service (Lee et al., 2015)....al., 2017). In addition to additional elements for S. cerevisiae chromosomal integration and gene expression...//doi.org/10.1021/acssynbio.9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling...

16. What's New in CRISPR - Winter 2018

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    ...precise deletion products compared to monomeric Cas9’s. Read the paper in Nature Communications Find ...

17. Ras Pathway

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    ...targets. Rajalingam K, Schreck R, Rapp UR, Albert S. Biochim Biophys Acta. 2007 Aug;1773(8):1177-95. PubMed...

18. Twenty Years of Sharing: Addgene's Viral Vector Service

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    ...Matho, K. S., Cetin, A., Woodard, K., Cepko, C., Guerin, K. I., Rego, M. A., Ersing, I., Bachle, S. M., Kamens...

19. Antibodies 101: Introduction to Immunofluorescence

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    ...References and resources References Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to Performing...fluorescence-microscopy/   Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez...

20. The Fluorescent Vegetables in Aptamer Soup

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    ...2412-2420. PubMed PMID: 25101481. Filonov, Grigory S., et al. "Broccoli: rapid selection of an RNA mimic...Central PMCID: PMC4923196. Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification and characterization...