We narrowed to 835 results for: ANG

...three additional amino acid changes were introduced to nCas9. These changes promote nCas9’s dissociation...molecular biologists use to tinker with DNA. Making changes to a DNA sequence can help scientists identify ...types of nucleotides. Both the Bassik Lab and the Chang Lab have created methods that introduce diversification...Cytidine Deaminase (AID) enzyme. AID, however, only changes cytidines or guanines to the other three bases,...

...our understanding of what accessibility is would change over the next twenty years. But learn we did! ... of Addgene’s content team, we — Jo, Brook, and Angela — most often think about accessibility in terms... and educational content conflicted with plain language guidelines.  Which is why, at the Society for ...when I’m done? What are we trying to fundamentally change about their knowledge?    Here, this image is being.... Other times, it is more helpful to use plain language and focus on a broad takeaway.  In this image,...

...Dolan, E., Wang, Hou, Zhonggang, Xiao, Yibei, et. al. Introducing a Spectrum of Long-Range Genomic Deletions...an expanding number of others. Best for: a wide range of DNA edits.   Cas3 – The shredder What sets Cas3... sticky ends. Cas12a cleavage results in 5 bp overhangs and is particularly convenient for targeting experiments...

...fluorescent protein tools. This year, we’ve made some changes. In addition to supporting in-person conference...health and disease,” says Aggarwal. This biosensor changes fluorescence based on the release of glutamate ...be transferred to the roGFP2 cysteine thiols and change its fluorescence properties. The biosensor can ...allow us to better understand how neuron activity changes following opioid withdrawal, providing insight ... Jennifer Tsang...

...neuroendocrine, adipose, immune, inflammatory, angiogenic vascular, endothelial, and stromal cells - a ...lack of caveolin 1 in breast cancer also induces changes of stromal cells in the microenvironment. This ...transferred to other cells, inducing epigenetic changes in targeted cells. In this case, exosomes may transport...formation by inhibiting cell growth, invasion, and angiogenesis (Allinen et al., 2004). In fact, Sanford H. ...influence tumor microenvironments: immunology and angiogenesis (Witz et al., 2009). Then, scientists began ...

...go ahead with raises. What will these further changes mean? The abrupt reversal in salary raises at institutions...Continuing efforts to gather information and expedite change We are continuing to update our FLSA and Postdocs..., under “How institutional plans have/have not changed since the injunction”. Postdocs around the country...enterprise and promote their voices in driving changes in science. Follow him on Twitter @BiophysicalFrog...the Addgene Blog Read Gary's Previous Article: Changing Labor Laws Bring Increased Postdoc Wages Read ...

...quick point-of-care diagnosis kits (reviewed in Tsang and LaManna, 2020). To see even more tools, head...and resources References Biering SB,  Sarnik SA, Wang E, Zengel JR, Sathyan V, Nguyenla X, Van Dis E, ..., Johnson NV, Ludes-Meyers J, Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard ...bioRxiv. https://doi.org/10.1101/2021.03.10.434840 Tsang J, LaManna CM (2020) Open Sharing During COVID-19... Jennifer Tsang...

...precise insertion of a DNA sequence ranging from a single nucleotide change to large insertions like the addition...: The key components of Nanoblades. Image from Mangeot et al., 2019.   MLV GAG-POL polyprotein Nanoblades...efficiencies of the chimeric and fully albino mice ranging from 11% to 100%, a level similar to other approaches... accessible genome editing tool.   References Mangeot, Philippe E., et al. "Genome editing in primary...

...viral components; according the manufacturer these changes result in yields up to 25x higher than other available...such as Cas9. See figure 1 for an example of how changing transfection ratios can affect transfection efficiency...assessing lentiviral titer, don't compare apples to oranges After choosing a packaging system and producing...line. To overcome this issue, we recommend using a range of doses of lentivirus when transducing a target... Oct 10;12(15):1893-905. PubMed PMID: 11589831. Jiang W. et. al.. An optimized method for high-titer lentivirus...

...may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try amplifying the...working with virus: 1. Viral vectors undergo DNA rearrangements. Depending on the composition of the viral...viral vector with bacteria designed to minimize rearrangements such as NEB Stable or use a standard strain... following transfection. In other words, when exchanging the medium after transfecting cells, replace ...

..., a deletion or a frameshift (Ding et al., 2014, Wang et al., 2015). In these studies, the strategy employed...disruption frequencies in the various cell types ranged from 18% to 65% (Maggio et al., 2014). An alternative...and Adeno EA. Or if you're looking for a broader range of CRISPRs plasmid tools, find more plasmids, CRISPR...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature 516:423... . https://doi.org/10.1016/j.jconrel.2019.02.009 Wang D, Mou H, Li S, Li Y, Hough S, Tran K, Li J, Yin...

...in other plant species, thanks to the broad host range of TRV. Find TRV-TnpB plasmids here! Weiss, T.,...transsynaptic label based on antibody-like sensors) changes that. Developed by the Arnold Lab, ATLAS is based...subunit GluA1 found at excitatory synapses (Rivera & Huang, et al., 2025). AF was fused to a presynaptic vesicle...generated with mRNA display. Modified from Rivera & Huang, et al. (2025) under CC BY-NC-ND license, created...applications. Find ATLAS plasmids here! Rivera, J. F., Huang, H., et al. (2025). ATLAS: a rationally designed...

...bacterial strain, such as a B. subtilis, or a narrow range of bacterial species, such as the human gut bacteria...proteins. Second, it can be introduced into a broader range of bacteria. Once transferred, Mobile-CRISPRi stably...reporter, was variable across bacterial strains, ranging from an ~8 to 150-fold reduction, with a median...been a target for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible...27381852. Choi, Kyoung-Hee, et al. "A Tn7-based broad-range bacterial cloning and expression system." Nature...

... to create xCas9, an enzyme recognizing a broad range of PAMs like NG, GAA, and GAT, but also displaying...spectinomycin resistance. xCas9 3.7 showed the broadest PAM range, recognizing NG, NNG, GAT, and CAA PAMs, while xCas9... xCas9 3.6 displayed the second best PAM range. Characterizing xCas9 in mammalian cells Hu et al. compared... the observed off-target loci displayed a broad range of PAM sequences, indicating they may be less predictable... with NGS) are shown as percentages, with fold changes compared to SpCas9 in parentheses. The development...

...enjoy seeing how our quality control process has changed with the introduction of next generation sequencing... we use blastn to identify any discrepancies in Sanger sequences, such as mismatches, deletions, or insertions... first decision to make is whether to compare a Sanger sequencing result to a single known reference sequence...95% of our results were always correct!]  Since Sanger sequencing reactions are imperfect and sequence...used blastn to determine the reliable portion of a Sanger sequencing result and noted any potential mismatches...

...instead of nMagHigh1-C714, generating paCas9-2. This change did not significantly alter the system’s efficiency...Using Guide RNAs with Photocleavable Protectors. Angew Chem Int Ed 55:12440–12444 . https://doi.org/10.1002...Biology. Springer US, pp 261–281 Manna D, Maji B, Gangopadhyay SA, Cox KJ, Zhou Q, Law BK, Mazitschek R, Choudhary...Dosing and Spatiotemporal Control of CRISPR‐Cas9. Angew Chem Int Ed 58:6285–6289 . https://doi.org/10.1002...Bardhan A, Delaney M, Ilk AS, Rauen RR, Kahn SI, Tsang M, Deiters A (2020) Spatiotemporal Control of CRISPR... and Zebrafish using Light‐Activated Guide RNA. Angew Chem Int Ed 59:8998–9003 . https://doi.org/10.1002...

...10.1073/pnas.76.2.615  Zhou, Y., Zhang, Y., He, W., Wang, J., Peng, F., Huang, L., Zhao, S., & Deng, W. (2018...the Patterson Protocol was shown to purify DNA ranging in size from 100 bp to 48 kb with high yields and...10.1038/s41598-018-30316-w  Zou, Y., Mason, M.G., Wang, Y., Wee, E.J., Turni, C., Blackall, P.J., Trau,...

...have larger homology arms in the 500 bp to 1 kb range, however, shorter homology tracts have recently ...is a silent mutation, so you don’t accidentally change your protein of interest.   Fig. 2: Stop...to be efficacious for increasing knock-in events (Yang et al., Int J Mol Sci). Adding these inhibitors ...cutting. Cas12a may promote HDR by generating ssDNA overhangs at the break site – an early HR step (Moreno-Mateos...: 10524-10535 (2018). 1 10.1074/jbc.TM118.000372 Yang, H., Ren, S., Yu, S., Pan, H., et al. Methods favoring...

...regulations. A grant professional’s job duties can range from coordinating the grant application and submitting...government grantmakers. We keep up-to-date on the changing guidelines and regulations impacting grant management...government. Throughout these moves and program changes, I learned to write grants for health, housing,...within the city infrastructure. My career path changed when I was asked if I would consider consulting...evaluation and grant management.  Your niche will change over time. Going into the grants profession with...

...technology? See our improved CRISPR-Cas Guide. Wang et al., Science. 2014. Jan 3;343(6166):80-4. Shalem...a mutant of the fluorescent protein dsRed that changes irreversibly its color from green to red when it... show its usefulness in cell culture to report changes in mitochondrial turnover and transport. pMitoTimer...green) protein and organelles with older (yellow to orange) protein. Image courtesy of Roberta Gottlieb. ...sites, and provides a modular system for easier exchange and re-use of existing elements. DNA sequences... and Dieter Söll at Yale University previously changed the way researchers can explore important questions...site, and permits DNA fragments with compatible overhangs to be efficiently assembled. Scientists can engineer...