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  2. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...hairpin that can be designed to bind and detect nearly any RNA sequence. Downstream of the hairpin is ...26615910. PubMed Central PMCID: PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian...

  3. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...better serve the community. We are delighted at the early outcomes and the ecstatic reception by scientists...worry about getting support from depositors. We regularly hear how much they see the value in depositing...

  5. Addgene: The First Twenty Years

    Type
    Blog Post
    ...School. We want to give a special thanks to the early-adopter scientists, who trusted Addgene before we...— 10,000 plasmids deposited and available — and nearly seven before we had 1,000 depositing scientists...

  6. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ... also be used to ensure specificity; this is particularly helpful when wanting to target specific neuron...Dystrophy. InTech. doi: 10.5772/53023 Veldwijk, Marlon R., et al. "Development and optimization of a real-time...

  7. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...Cas12a cleavage results in 5 bp overhangs and is particularly convenient for targeting experiments requiring...it targets RNA instead of DNA. This makes it particularly useful in generating transient edits of signaling...

  8. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...Why study neural connectivity? One of the early lessons many of us learned in biology is that the body...Deshpande et al., 2013). The study found that at early time points, most of the projections to these new...

  10. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ... direct ELISA, aside from the detection step. Similarly, the protein antigen is coated onto the wells ...calculate the protein concentration. Image from Jagarlamudi et al., 2015.   The maximum OD that most ...

  12. A Look at Addgene's QC Process

    Type
    Blog Post
    ...differences are considered a QC issue is reviewed yearly at a best practices meeting, where the QC team ...pipeline. There are regions of plasmids that are particularly difficult to sequence and assemble using the...

  13. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...Zibitt et al. 2021). Mutating splice sites is particularly effective as it can result in the retention ...used to target lncRNAs with high specificity, particularly in applications requiring smaller delivery systems...

  15. 10 Basic tips for mammalian cell culture

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    Blog Post
    ... close to the hood so you can view your cells regularly (both before entering the hood and when putting...Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology and Immunology. You...

  16. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7... and base editing, to more loci. Although these early data are exciting, there’s a lot we still don’t ...

  17. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...experience, the more I heard that getting into industry early would only help. Like any major life decision, ...could be laid off while you’re there. Of course, similarly, an academic advisor could also lose their funding...

  19. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...dsDNA in most starting materials, and is not particularly sensitive. The fluorescent signal can be affected...be affected by DNA quality and primer design, particularly for amplicons under 100 bp (Ma, 2015.) A schematic...

  20. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...may be too constraining for some experiments, particularly in vivo ones. In those cases, AAV vector screens...your CRISPR screen to a high degree. This is particularly helpful when investigating tissue-specific diseases...
Showing: 241 - 260 of 576 results