...zebrafish Whole organism lineage tracing by combinatorial and cumulative genome editing - Jay Shendure...America. The Zebrafish Book - A guide for the laboratory use of zebrafish. CRISPRz - A database of CRISPR...

...Addgene contracted with a professional scientific translator, L. Denise Roche, to edit the translation. We...  Many thanks to:  Daniel Mejías Sánchez, PhD, translator  Denise Roche, PhD, MD, editor, ldeniseroche@...

.... While certain cell types, such as principal excitatory cells and glia, have been successfully targeted...’s lab has developed a new AAV approach using regulatory elements that restrict expression to this cell...wild type SOX2 with either a SOX2-VP16 (strong activator) or SOX2-HP1 (strong repressor) fusion protein...

... Singapore. Chapter 4: Life as a new primary investigator. Coming up with new project ideas, applying ... Chew! Wei Leong Chew is a Junior Principal Investigator at the Genome Institute of Singapore. His lab...

...structure is strikingly similar to the design model at atomic resolution” (Kuhlman and Dantas et al., 2003). ...). Design of a Novel Globular Protein Fold with Atomic-Level Accuracy. Science, 302(5649), 1364–1368. ...

... retrograde serotype, and a dTomato under the control of the E6 regulatory element, which targets it to...

...Cell demonstrate how PGC-1a1, a transcriptional coactivator induced in skeletal muscle by exercise and endurance...altogether. Their muscles showed an increase in inflammatory cytokines as a result of those mild stressors...

...elegans Dupuy Lab and Lehner Lab vectors - Tools for nematode transgenesis Insect / Baculovirus Polyhedrin pFastBac...vivo Dupuy Lab and Lehner Lab vectors - Tools for nematode transgenesis Rosenberg Lab Plasmids...

...Sun et al. Nat Methods. 2020 Oct 21. Glutamate indicators with improved activation kinetics and localization...with support from the BRAIN Initiative. Jackson Laboratory (Link opens in a new window) A collection of ...

...included the Belmont Human Rights Commission and mediator for the Massachusetts small claims court summary...Oncology and the Chicago Marathon to benefit Neurofibromatosis, New England. Additional resources on the...

..., from zinc finger nucleases to transcription activator-like effector nucleases (TALENs). Our most popular... GCaMP6 series of genetically encoded calcium indicators were the hot technology of the year in our collection...

...customizable DNA-binding domains based on transcription-activator-like effectors (TALEs) as anchors for genome engineering...and modular,” Konermann said. “Transcriptional activators are easy to expand or combine with different ...

... bit of a Twitter storm surrounding NgAgo, its creator, Han Chunyu of Hebei University of Science and ...Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect Science...

...environments, for example. “We’ve been contacted by investigators all over the world who are interested to use...researchers have plans to expand the library to include activators for more cancer-related pathways and more nodes...

...hour, we feasted on sliders, hot dogs and a mashed potato bar (complete with all the fixings), as well as...about her grandfather’s humble beginnings as an operator of a printing press. Just as her grandfather used...

...TCTTTTTGTGCTGCGCCAcccagcccataatcgaGTCTTCTACAGGCATGAA retinitis pigmentosa GTPase regulator b TAL3528 & TAL3529 TGGAGAAACAGAAGATGAaatccccgagtccggaGCTGTCTTCACTTTTGGA...TGGAGAAACAGAAGATGAaatccccgagtccggaGCTGTCTTCACTTTTGGA retinitis pigmentosa GTPase regulator b ORF15 TAL3530 & TAL3531 TAAGAGGGCGGAGCTTTTatctcgcaaagctctgCCCACTGAGTTACTTAGA...

...this purpose? What led you to it? Howarth: Our collaborator Uli Schwarz-Linek at St. Andrews discovered ... are useful for mechanical study of proteins by atomic force microscopy (AFM). Maybe polyantibodies could...

...for students, postdocs, and young Principal Investigators (PIs). We've interviewed Addgene depositors ...different ways to succeed – as loner or gregarious;  dictator or laissez-faire; theory or practice; business-like...

...metadata for each screen is captured by BioGRID curators, including the cell line/tissue type, enzyme system...CRISPR database are encouraged to contact BioGRID curators at [email protected] to submit their published...

...applications. Also, purifying GST by affinity chromatography requires proper folding of the tag, which prevents...to be purified by single step metal affinity chromatography. Purification of His fusion proteins from E...