We narrowed to 702 results for: BASE;

...a DNA-binding domain to recognize any desired 15 base pair sequence in the genome in rapid fashion. The...All you do to create a protein that binds those 15 base pairs is take four plasmids out of the library in...genome-editing system for generating human stem cell-based disease models." Cell Stem Cell. 12, 238-251 (7 ...

...and what work he's planning on doing with CRISPR-based gene drives focusing on safety and the ability to...Board with a particular interest in generating Cas9 based tools to enable facile control of DNA and RNA.   ...

... performing either PCR - or restriction enzyme -based cloning to test individual clones before use of ... what it should be. A useful restriction enzyme based technique for verifying plasmids like this is "plasmid...

...type of centrifuge needed. This decision should be based on the size of tubes you are using and the speed...than water, then you may need to fill the blank based on weight instead of volume. Pro-Tip For microfuge...

... knowledge base is going to necessarily need a diverse group of people to provide that base. Sometimes... Mission accomplished! One example of a mission-based team in academia would be your school’s post-doc...Let’s think about some of the advantages of team-based work: Nobody knows everything anymore John F. Kennedy... post, so here are just a few critical features based on my own experience: Leadership Few things are ...

...infectious titer of a viral vector stock can vary based on transduction target and can also be altered by...titer is typically calculated by two popular PCR-based methods: Quantitative PCR (qPCR) Digital droplet...not widely used today (Fagone et al., 2012). PCR-based methods are robust, easy, fast, and convenient. ...quantified with a different primer pair. Ct values vary based on the amount of starting material in the PCR because...

... et al) we were very excited to set up a CRISPR-based editing platform in our lab. Our goal was to use...binding sequence, we recommend changing several bases as close to the PAM as possible (see below to find...Ludwig-Maximilians-University Munich. His lab develops iPSC-based models of neurodegenerative and neurovascular diseases...of Alzheimer’s disease by developing novel iPSC-based model systems.     References 1. Cong, Le, et al...

...are functionally differentiated from one another. Based on their structural elements, oris interact with...proteins within different host organisms. pBBR1-based plasmids and plasmids in incompatibility groups ...campestris pv. malvacearum, Zymomonas mobilis pBBR1 based pBBR1  Gram-negative  Alcaligenes eutrophus...constitutive and IPTG-inducible integron promoter-based expression systems for Escherichia coli and Agrobacterium...

...of the desired mutation (usually 1-3 mismatched bases) is sufficient for your primers to successfully ...a minor extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction...feature used in TA-cloning). Such non-complementary bases at the 3’-end interfere with plasmid reconstitution...et al. "Predicting DNA duplex stability from the base sequence." Proceedings of the National Academy of...

...the best possible results for any tissue culture-based experiment, labs should routinely test for mycoplasma...detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels of contamination...detection, Uphoff et. al. outline a do it yourself PCR-based approach complete with freely available positive...concentration and titration of pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009):...

... decisions based on experimental timelines and specific aims. 2. Then make decisions based on financial...instructions on creating a budget, making decisions based on that budget, and negotiating in regards to the...will need, and how long it will take to publish based on these findings. Most often we suggest estimating...

...contributed by guest blogger, Rachel Rubinstein, a field-based software product manager at Oxford Nanopore Technologies... messy if you’re not on top of it. In a science-based software PM role, having a good understanding of...Rubinstein! Rachel Rubinstein is currently a Field-based Product Manager at Oxford Nanopore Technologies ...

...of imaging data (ILASTIK), or the popular plugin-based image process framework FIJI are just three of the...to the biological protocol, and assembly designs based around the use of commercially available tools and...provides a potential conflict between traditional IP-based models and the use of taxpayer money to support ...

...allows classification of individual target molecules based on their mutation status. The drop-off probe is ...References 1. Miyaoka, Y. et al. Isolation of single-base genome-edited human iPS cells without antibiotic..., Berman, J. R. & Postovit, L.-M. A Digital PCR-Based Method for Efficient and Highly Specific Screening...

... efficiencies ranking as low, medium or high is based on the reprogramming efficiencies reported in González...Non-replicating Plasmids: Generating iPSCs with plasmid-based expression requires serial transfection of 1 or ...Replicating oriP/Epstein-Barr nuclear antigen-1 (EBNA1) based episomes: These plasmids carry the origin of replication...DNA methylation. Another downside of using EBNA1 based vectors is that the plasmid can hang around for ...behave. iPSCs are typically tested with molecular-based assays first. These methods less rigorously assesses...Transgene Expression Mediated by a Sendai Virus-Based Vector Using a Nonstructural C Protein and Endogenous...

...released report “Enhancing Dissemination of Evidence-Based Models for STEM PhD Career Development; a Stakeholder...development and implementation of collections of evidence-based practices. These concrete programs and practices...

... of DNA you need, and at which level of purity. Based on these premises we can classify a plasmid preparation...will adopt for the extraction. Restriction enzyme based approaches can require several hundreds of nanograms...

... your website? Many vendors will process orders based on a purchase order, which is an official document...depositing institutions. Addgene cannot fulfill orders based on PO documents that are sent to us by fax or email...

...resistance cassette) in pUC19 based entry vectors, as well as the pGreen-IIS based destination vectors. To ...CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing a means...ACS Synth Biol 2013 Sep 20;2(9):537-41. DREADD-based chemogenetic technologies After several years...up to six DNA fragments at a time. This method (based on the Golden Gate technology) exploits the ability...reconstitution across synaptic partners) technique based on functional complementation between two non-fluorescent...tags in the FP sequence for separation from Cre-based Brainbow-expressing cells. Autobow plasmids are ...The expression plasmids for a given species are based on the same backbone to permit expression levels...

...ends are compatible, meaning that the overhanging base pairs on the vector and insert are complementary... to adjust the amount of DNA used in a ligation based on the length of the DNA to get a proper ratio of...