...of imaging data (ILASTIK), or the popular plugin-based image process framework FIJI are just three of the...to the biological protocol, and assembly designs based around the use of commercially available tools and...provides a potential conflict between traditional IP-based models and the use of taxpayer money to support ...

...contributed by guest blogger, Rachel Rubinstein, a field-based software product manager at Oxford Nanopore Technologies... messy if you’re not on top of it. In a science-based software PM role, having a good understanding of...Rubinstein! Rachel Rubinstein is currently a Field-based Product Manager at Oxford Nanopore Technologies ...

... performing either PCR - or restriction enzyme -based cloning to test individual clones before use of ... what it should be. A useful restriction enzyme based technique for verifying plasmids like this is "plasmid...

...type of centrifuge needed. This decision should be based on the size of tubes you are using and the speed...than water, then you may need to fill the blank based on weight instead of volume. Pro-Tip For microfuge...

...Resources Our curated resources group plasmids together based on type or application. Browse our guides to find...

...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ...

...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ...degradation. This degradation can continue for many kilobases in one or both directions from the target region...

...manuscript submitted), and the FLASH (Fast Ligation-based Automatable Solid-phase High-throughput) assembly...

... efficiencies ranking as low, medium or high is based on the reprogramming efficiencies reported in González...Non-replicating Plasmids: Generating iPSCs with plasmid-based expression requires serial transfection of 1 or ...Replicating oriP/Epstein-Barr nuclear antigen-1 (EBNA1) based episomes: These plasmids carry the origin of replication...DNA methylation. Another downside of using EBNA1 based vectors is that the plasmid can hang around for ...behave. iPSCs are typically tested with molecular-based assays first. These methods less rigorously assesses...Transgene Expression Mediated by a Sendai Virus-Based Vector Using a Nonstructural C Protein and Endogenous...

... of DNA you need, and at which level of purity. Based on these premises we can classify a plasmid preparation...will adopt for the extraction. Restriction enzyme based approaches can require several hundreds of nanograms...

...released report “Enhancing Dissemination of Evidence-Based Models for STEM PhD Career Development; a Stakeholder...development and implementation of collections of evidence-based practices. These concrete programs and practices...

... your website? Many vendors will process orders based on a purchase order, which is an official document...depositing institutions. Addgene cannot fulfill orders based on PO documents that are sent to us by fax or email...

...resistance cassette) in pUC19 based entry vectors, as well as the pGreen-IIS based destination vectors. To ...CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing a means...ACS Synth Biol 2013 Sep 20;2(9):537-41. DREADD-based chemogenetic technologies After several years...up to six DNA fragments at a time. This method (based on the Golden Gate technology) exploits the ability...reconstitution across synaptic partners) technique based on functional complementation between two non-fluorescent...tags in the FP sequence for separation from Cre-based Brainbow-expressing cells. Autobow plasmids are ...The expression plasmids for a given species are based on the same backbone to permit expression levels...

...into a maxi-prep culture. Prep size may be scaled based on the required DNA yield. Perform maxi-prep for...another transfection method such as cationic liposome-based transfection. Optimize transfection conditions for...Optimize PCR conditions for each primer pair designed based on testing the bulk sorted cells. Run samples on...

...change, so they are typically used in microscopy-based assays like immunohistochemistry, though they are....  Biotin Biotin is used in a range of antibody-based assays. Biotin itself is not visible or capable ...But hopefully, this introduction provides a good base from which to dive deeper!References and resources...instruments, each other, and your target. I love FPbase’s Spectra Viewer tool for comparing different fluorophores...

...resolution for in vivo work. APEX2, a faster peroxidase-based labeling approach, is an alternative option but ...signaling. Limitations of RaPID Like other BirA*-based proximity labeling methods, RaPID cannot distinguish...reported by other research groups that performed BirA*-based affinity purification mass spec experiments. These...

...protein degradation. Enter TRIM-Away - an antibody-based system developed by the James and Schuh labs. Clift...endocytosis is further complicated by the reliance on pH-based biosensors used to detect the maturation of neutral... Crabtree’s lab has developed a new CRISPR/Cas9-based system to study epigenetic modulation. While catalytically...

...recombination of the targeting vector. CRISPR/Cas9-based systems have been developed for generating knockin... for gestation. All three can introduce multiple base pair deletions, but CRISPR/Cas9 can also introduce...in which the conditional recombination can occur based on the cells in which Cre is expressed.  Several...

...is less than half the size of the Cas9 or Cas12a based systems, making it well suited for AAV packaging...was shown to be effective for CRISPR activation, base editing, and genome editing in mammalian cells. ...had the highest activity for gene activation  and base editing, whereas a different set of mutations (the...

...gradient with different concentrations of your protein-based buffer (FBS, milk, etc.) against the same amount...instead of concentrations. Most labs use protein-based buffers, so here’s a quick tip: Keep a bottle of...even give gelatin a shot.  Remember that protein-based blockers degrade fairly quickly, even at 4 °C, so...peroxidase protein (HRP), have a limited dynamic range based on the solution used to expose them. You’ll need...(if available) and note in analysis Plasmid-based proteins: plasmid sequence not accurate Check ...and/or concentration Try a different protein-based buffer (eg, milk instead of BSA) Nonspecific...buffer/adjust lysing buffer strength Sonication based lysing protocol Subcellular fractional kit or...