We narrowed to 381 results for: BRE

1. Mentoring for Scientists: I Have a Mentor, What Now?

   Type

   Blog Post

   ...Figure out what I want to do after I get my PhD) and break them down into smaller, actionable steps (e.g. Meet...

2. Reagent Repositories Are Speeding up Science During the Pandemic

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   Blog Post

   ...doi.org/10.1016/j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038...

3. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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   Blog Post

   ...presenter at their poster, they could be hosted in breakout rooms, or even on social media.Zhang was able ...

4. Networking Follow-up: I Met a Person. Now What?

   Type

   Blog Post

   ... multiple times if you don’t get a response. It breaks an important law of networking--don’t be creepy...

5. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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   Blog Post

   ...Addgene. The Podgorski Lab has provided a brief breakdown of the promoter systems you can use to express...

6. The Power Behind NGS Plasmid Validation: seqWell

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   Blog Post

   ... seqWell Inc. Plasmids and PCR products are the bread and butter of molecular biology labs the world over...

7. Four Factors that Differentiate the Stem Cell Field

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   Blog Post

   ...Parkinson's Disease; Hideyuki Okano presented ground-breaking work on spinal cord injuries; Jessica Young shared...

8. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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   Blog Post

   ...given application is a challenge. To help keep you abreast of the latest fluorescent proteins, I maintain ...

9. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   Blog Post

   ...only able to enter the nucleus during mitotic breakdown of the nuclear envelope. Lentiviruses can transduce...

10. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type

    Blog Post

    ...be targeted by a sgRNA guided Cas9 causing a DSB break. This DSB is repaired by NHEJ which depending on...

11. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    Blog Post

    ...the DNA base change without a DNA double-strand break. But base editors aren’t perfect. They may be slow...

12. Sweating the Small Stuff: Details in the Lab

    Type

    Blog Post

    ...intentionally slow down, you’re giving yourself a chance to break what can be a rather vicious cycle.  Everybody’s...

13. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post

    ...the histone methylation status at a double-strand break that needs repairing. They may be looking at chromatin...

14. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    Blog Post

    ...Comprehensive Set of MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell Reports, 2016. PubMed PMID: 27760319...

15. Targeted Mutagenesis with EvolvR

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    Blog Post

    ...tracing cells that do not tolerate double-strand DNA breaks by introducing a unique tag into a genomic site...

16. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

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    Blog Post

    ... it available to everyone, that creates more breakthrough and innovation. With any kind of open platform...

17. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    Blog Post

    ...These cells, presumably the cell you want, cannot break down X-gal and are white. Cells that don’t contain...

18. Building and Validating Recombinant Antibodies: A Case Study

    Type

    Blog Post

    ... Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. A toolbox of IgG subclass-switched...

19. Viral Vectors 101: Viral Vector Elements

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    Blog Post

    ...components are called helper plasmids. Below, we’ll breakdown the vector components first by virus and then ...

20. Viral Vectors 101: Types of viruses

    Type

    Blog Post

    ...through nuclear pores and thus do not require breakdown of the nuclear envelope during cell division to...