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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 802 results for: Clu

Showing: 241 - 260 of 802 results

  3. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...protease. The carboxyl-terminus of the fusion protein includes an AviTag and a 6X HisTag for affinity purification...purification (see plasmid map and cartoon below). Including biotin in your culture and inducing protein expression...binding kinetics using a multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences...

  4. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ... by any number of characteristics. Common ones include age range, familiarity with the subject, shared... that by defining a specific audience, they’ll exclude others from accessing their work. But that’s not...science communication work does not explicitly exclude people outside of the original recipients. Instead...

  5. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...allow researchers to understand bacterial gene clusters that produce useful natural materials, such as...are some highlights from the past couple months including several pooled libraries: Cas13X and Cas13Y, ...sensors from Yulong Li’s lab are now available including: pAAV-hsyn-GRAB_DA2h in AAV9 New GRAB-DA and ...

  6. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...thickness. The fibers are drawn with microelectronics included in them for downstream control – this is important...components are added - the ‘bells and whistles’ – including microscale light emitting diodes, thermal sensors...neuroscience tools into the brains of model organisms, including the Anikeeva lab’s work on a similar neural fiber...

  7. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...structure and transparency of methods sections, including clear information about reagents, and why other...roles on review and primary research journals including Trends in Cell Biology, Cell, Molecular Cell, ...inception and launch of other Cell Press journals, including Cell Metabolism, Cell Reports and Joule. In September...

  8. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...navel-gaze over from the last couple of weeks, including whether or not scientists can recognize so-called...work on emerging issues in novel technologies, including self-driving vehicles, synthetic biology, biosecurity...factory with their cats, Harley and Lexi, and a clutter ofoutdoors-only spiders, all of whom are named ...

  9. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...protein, etc...).  There are many online guides – including an excellent series of posts by Joachim Goedhart...risk of inaccurate data submissions, so FPbase includes a versioning system that allows users to see when...improvement and elaboration.  Current projects include incorporation of biosensors, protein evolution ...

  10. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ... planning to soon expand to other cell types, including more physiologically-relevant systems, to catch... viral proteins except for Nsp3 and Nsp16, and includes an additional catalytically dead version of the...the viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier portability, ...

  12. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...lifesaver when your research requires cell culture and include a counter, lab timer, dilution calculator, and ...quite helpful and provides a list of directions including which buffers to use and that indicates if a sequential...reagents you work with in experiments. Hence, I’m including this MSDS app. The Chemical Safety Data Sheets...

  13. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ... Tom Ellis was offered a start-up package that included a postdoc and a PhD student in his first year....gradually incorporated regular group meetings, journal clubs, and focused sub-group meetings to discuss day-to-day... great resources for Early Career Scientists, including their "Making the Right Moves: A Practical Guide...

  14. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...previously identified Cas13a-c orthologs, but they do include HEPN nuclease domains characteristic of the Cas13...these studies also identified other advantages, including Cas13d-specific regulatory proteins and high targeting... knockdown by CasRx for each of four targets, including two mRNAs and two nuclear long non-coding RNAs...

  15. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...nano-lanterns for use in various applications, including optogenetics, biosensors, and fusion proteins....chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. In 2012...al. expanded the Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these...

  16. Hot Plasmids - February 2022

    Type
    Blog Post
    ...applications. This set of new anti-FLAG frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and HaloTag ...HaloTag fusions, while the anti-HA frankenbodies include mEGFP, mCherry, and HaloTag. Figure 1: (a) Anti-FLAG...frankenbodies have a variety of applications, including live-cell imaging, single-molecule tracking, immunolabeling...

  17. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ... fluorescence in the presence of calcium. This includes FRET-based sensors such as Twitch. While calcium...world, there are sensors available for other ions including magnesium, potassium, and zinc. These ion sensors...sensors designed for them. For example, this class includes tension monitors associated with specific proteins...

  18. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...target a particular DNA sequence? Musunuru: The kit includes 832 plasmids containing prefabricated pieces that...the kit have the rest of the protein in place including FokI endonuclease domain as well as fluorescent...use as testified by the fact that the TALEN kit includes 834 plasmids for the pieces you need to assemble...

  19. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...an epitope folded into a space that would size-exclude an antibody. In imaging applications, smaller affinity...They are often used for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed...molecules can bind to a wide variety of targets, including proteins, peptides, amino acids, drugs, metal ...

  20. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ... antibodies available (others you’ll encounter include monoclonal and recombinant antibodies). By the ...further antibody production, polyclonal antibodies include any antibodies produced during the immune response...staining you are seeing is real. It’s important to include a negative control using serum from the same species...
Showing: 241 - 260 of 802 results