...for half of the trip. Preparations ahead of time included contacting YNP staff to arrange permits to visit...frozen after collection. Figure 1: Paul included a voltmeter on top of the battery box to monitor...route that would reach all of the remote areas, including one hot spring requiring an overnight hike in....

...thickness. The fibers are drawn with microelectronics included in them for downstream control – this is important...components are added - the ‘bells and whistles’ – including microscale light emitting diodes, thermal sensors...neuroscience tools into the brains of model organisms, including the Anikeeva lab’s work on a similar neural fiber...

...allow researchers to understand bacterial gene clusters that produce useful natural materials, such as...are some highlights from the past couple months including several pooled libraries: Cas13X and Cas13Y, ...sensors from Yulong Li’s lab are now available including: pAAV-hsyn-GRAB_DA2h in AAV9 New GRAB-DA and ...

... by any number of characteristics. Common ones include age range, familiarity with the subject, shared... that by defining a specific audience, they’ll exclude others from accessing their work. But that’s not...science communication work does not explicitly exclude people outside of the original recipients. Instead...

...the mid-20th century, and many iconic papers, including the ones on the structure of the DNA, were not...first couple of sentences - even if written in a clumsy language make it much easier for me to go back ...initial read, adjust my language in the first (and clunky) version of my general comments and adjust them...

...lifesaver when your research requires cell culture and include a counter, lab timer, dilution calculator, and ...quite helpful and provides a list of directions including which buffers to use and that indicates if a sequential...reagents you work with in experiments. Hence, I’m including this MSDS app. The Chemical Safety Data Sheets...

...protein, etc...).  There are many online guides – including an excellent series of posts by Joachim Goedhart...risk of inaccurate data submissions, so FPbase includes a versioning system that allows users to see when...improvement and elaboration.  Current projects include incorporation of biosensors, protein evolution ...

...navel-gaze over from the last couple of weeks, including whether or not scientists can recognize so-called...work on emerging issues in novel technologies, including self-driving vehicles, synthetic biology, biosecurity...factory with their cats, Harley and Lexi, and a clutter ofoutdoors-only spiders, all of whom are named ...

... planning to soon expand to other cell types, including more physiologically-relevant systems, to catch... viral proteins except for Nsp3 and Nsp16, and includes an additional catalytically dead version of the...the viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier portability, ...

...structure and transparency of methods sections, including clear information about reagents, and why other...roles on review and primary research journals including Trends in Cell Biology, Cell, Molecular Cell, ...inception and launch of other Cell Press journals, including Cell Metabolism, Cell Reports and Joule. In September...

...RNA-seq clustering of cells transduced with the TF pooled library. Louvain community clusters of individual...Collection) and an accompanying expression atlas, including over 3,500 human TF isoforms, to help researchers...

... Tom Ellis was offered a start-up package that included a postdoc and a PhD student in his first year....gradually incorporated regular group meetings, journal clubs, and focused sub-group meetings to discuss day-to-day... great resources for Early Career Scientists, including their "Making the Right Moves: A Practical Guide...

...nano-lanterns for use in various applications, including optogenetics, biosensors, and fusion proteins....chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. In 2012...al. expanded the Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these...

...previously identified Cas13a-c orthologs, but they do include HEPN nuclease domains characteristic of the Cas13...these studies also identified other advantages, including Cas13d-specific regulatory proteins and high targeting... knockdown by CasRx for each of four targets, including two mRNAs and two nuclear long non-coding RNAs...

... fluorescence in the presence of calcium. This includes FRET-based sensors such as Twitch. While calcium...world, there are sensors available for other ions including magnesium, potassium, and zinc. These ion sensors...sensors designed for them. For example, this class includes tension monitors associated with specific proteins...

...applications. This set of new anti-FLAG frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and HaloTag ...HaloTag fusions, while the anti-HA frankenbodies include mEGFP, mCherry, and HaloTag. Figure 1: (a) Anti-FLAG...frankenbodies have a variety of applications, including live-cell imaging, single-molecule tracking, immunolabeling...

...target a particular DNA sequence? Musunuru: The kit includes 832 plasmids containing prefabricated pieces that...the kit have the rest of the protein in place including FokI endonuclease domain as well as fluorescent...use as testified by the fact that the TALEN kit includes 834 plasmids for the pieces you need to assemble...

...undergrad, I was assigned a paper to give as a journal club to my molecular biology class. A Cell paper. With...took just under a minute. I can't say the journal club was a rousing success–the response was a full sixty...with you. If you can give a seminar, lead a journal club, or present at lab meetings, you’ve developed a ...

... antibodies available (others you’ll encounter include monoclonal and recombinant antibodies). By the ...further antibody production, polyclonal antibodies include any antibodies produced during the immune response...staining you are seeing is real. It’s important to include a negative control using serum from the same species...

...an epitope folded into a space that would size-exclude an antibody. In imaging applications, smaller affinity...They are often used for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed...molecules can bind to a wide variety of targets, including proteins, peptides, amino acids, drugs, metal ...