We narrowed to 372 results for: Fus

1. CRISPR in the Clinic

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   ...CRISPR targets — Sickle Cell Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which...

2. Viral Vectors 101: Chemogenetics

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   ...activated through administered drugs that must diffuse throughout the body, the onset of receptor activation...

3. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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   ... McLellan JS (2020) Structure-based design of prefusion-stabilized SARS-CoV-2 spikes. Science 369:1501...

4. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   ...spatial control of CRISPR-Cas activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows...

5. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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   ...transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab...

6. Mouse Modeling, Part 1: Genetically Engineered Mice

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   ...tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain of estrogen receptor...

7. Plasmids for Endogenous Gene Tagging in Human Cells

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   ... In addition to generating fluorescent protein fusions, plasmids are available for tagging with epitope...

8. Educational Planarian Tools for Teaching Developmental Biology Techniques

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   ...complicated. These strains are more picky eaters and refuse to eat liver paste mixed with E. coli. Thus, to...

9. Science communication: 9 Strategies to Get Your Foot in the Door

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   ...Sarah Schmidt is engineering resistance against Fusarium wilt disease in bananas as a Marie Curie Fellow...

10. Hot Plasmids and Viral Preps - January 2021

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    ...heterogeneous cultures.  HeLa cells expressed a fusion of keratin with mGold (top) and mVenus (bottom)...

11. Magnetic Control of Proteins: More than a Dream

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    ...fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling FlavinTag made a reliable...

12. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    ... a prime editor (typically a Cas9 H840A nickase fused to a reverse transcriptase) together with a prime...

13. PhD Applications After COVID

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    ...funded? Are there any financial aspects that are confusing? During a traditional campus visit, many students...

14. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ...Protein Purification RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody Toolbox Resources...

15. Tips to Make the Most of a Scientific Conference

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    ...your elevator pitch where the other person seemed confused, bored, or particularly excited about? If you ...

16. Plasmids 101: 5 factors to help you choose the right cloning method

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    ...products into E. coli. Ligases within the E. coli then fuse together the PCR products via overhangs left over...

17. 3D Printing Meets CRISPR Cas9

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    ... initial model of this protein – but were very confused because the HNH nuclease active site wasn’t even...

18. Cloning Mammalian Cells with the Agarose Method

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    ...to the gene of interest (or, if your protein is fused with a fluorescent protein), fluorescence microscopy...

19. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ... fly stocks and other reagents. If you’re ever confused about the best way to study YFG in flies, there...

20. Addgene's Cancer Collection Pages Connect the Research Community

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    ...different applications such as fluorescent protein fusion studies and the generation of mutant cell lines...