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  1. Of Myc and Men

    Type
    Blog Post
    ... those questions in this post as we explore… the life and times of the myc tag.  Origins of protein tags...would help the field explore Myc’s role in cell proliferation and tumorigenesis.  Using two different synthetic...

  3. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...Resources I asked some colleagues to share valuable life lessons they have learned during their scientific...wrote about them in a blog post on Born to Science: Life Lessons from Scientific Training More and more ...

  4. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...required by MMEJ can easily be added via PCR amplification. Given the inefficiency of HDR for knock-ins...Select for puromycin resistant cells Step 5: PCR amplify and sequence the locus to verify correct GFP-puro...

  5. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...two-step staining with antibodies. Nanobodies also simplify multicolor staining experiments because they don...epitope-mapping and native protein complex isolation. eLife. PubMed PMID: 26633879 PubMed Central PMCID: PMC4755751...

  9. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...photostable than GFP. In addition, antibody-based amplification of their signals is weak and increases background...sites for primary antibodies which can be used to amplify the direct smFP signal within sub-cellular structures...

  10. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...staining. Direct ELISAs also lack the signal amplification afforded by secondary antibodies and thus are... bind to a single primary antibody and thereby amplify the signal. Since the primary antibody does not...

  11. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...University and became a lecturer in the College of Life Sciences that same year. Her research is focused...studying biochemistry at the University of Southern California. He is also an intern at the Yang Lab of the ...

  13. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...knowledge of the software, sharing knowledge allows for life to happen without impacting the overall work. Each...and tidy it up. Conclusion While none of us are life science majors, we do our best to understand the...

  14. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...to that of a professional email. Notes from real-life hiring managers In preparation for updating this...that stage, cover letters can be a barrier for qualified applicants. In short, while a cover letter can...

  15. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...two other areas that could potentially improve the life expectancy of mesothelioma patients: early detection...use, they will have a much greater impact on the life expectancy and overall survival of mesothelioma ...

  16. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...possible, especially if the infection is serious or life-threatening, treatment will often begin before this...development of resistance; it’s a natural process of life and one which has allowed bacteria to evolve and...

  17. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...expertise in science careers, women in STEM, work-life negotiation, and mentorship, we decided to have ...questions about science careers, women in STEM, work-life negotiation, and mentorship. We can direct people...

  18. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...base pair sequence surrounding your gRNA. Once amplified, you can insert this region into a pCAG-EGXXFP...to the day you have your F0 generation (barring life’s little accidents and stuff ups). This is a huge...

  19. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...acceptor photobleaching method and the fluorescence lifetime imaging microscopy (FLIM) method. Acceptor photobleaching...not to destroy the donor molecule. Fluorescence lifetime imaging microscopy method FLIM has been developed...
Showing: 241 - 260 of 471 results