We narrowed to 365 results for: Pol;
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TypeBlog Post...to get going. It’s like being stuck in jail in Monopoly—you keep rolling the dice and not moving while...
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Celebrate National Book Month with Addgene
TypeBlog Post...(which had been lost for an age) fight in the Napoleonic Wars and against something even more sinister... -
Finding and Joining Your Dream Lab
TypeBlog Post...or contract, you should be starting to send out polite emails with your CV to people you’d like to work... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...III. Gene III is under the control of a T7 RNA polymerase and cannot be expressed until the base editor... -
AAV Q&A with Tim Miles
TypeBlog Post... before considering the need for some manner of polyA. In this scenario, you may be best served by a dual... -
Hot Plasmids - February 2022
TypeBlog Post...individually (~20,000 lanes/50 mLs) or through two polycistronic expression vectors (3570 lanes/50 mLs) coexpressing... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...at odd hours, used more secluded workspaces, or politely asked to not be disturbed when they were at the... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog Post...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies... -
Antibodies 101: Isotypes
TypeBlog Post...lot of ways to classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog Post...previously mentioned, enlist the help of your network to polish the final draft prior to submission. When in doubt... -
Communicating Your Science With Help From ComSciCon
TypeBlog Post...broader impacts for their work in education and policy and to draw attention and support to work in their... -
Plasmids 101: Inducible Promoters
TypeBlog Post...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ... -
Viral Vectors 101: Biosensors
TypeBlog Post...2016 Jun 30;60(1):1-8. DOI: 10.1042/EBC20150001 Leopold, A. V., Shcherbakova D. M., Verkhusha, V. V., Fluorescent... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post...exciting computational algorithms, such as SIFT or PolyPhen to approach this challenge, using attributes of... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog Post...PMCID: PMC3773023. 7. Vouillot, L., Thélie, A. & Pollet, N. Comparison of T7E1 and surveyor mismatch cleavage... -
A Deep Dive into BioBuilder
TypeBlog Post...whose work is focused on education and education policy. Additional Resources on the Addgene Blog Synthetic... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post.... Using commercially available high-fidelity polymerases such as KOD and Phusion, which are recommended... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog Post... have a higher conductance, so they can still depolarize neurons at lower MOIs. Figure 2: ChRgers... -
Summer SciComm: Preprints
TypeBlog Post...comment online! Want the general public (say, policymakers or community organizations) to use your work... -
dTAG - You're it!
TypeBlog Post...proximity with the target protein, leading to polyubiquitination of the target protein and proteasomal degradation...