...edit your gene of choice. These vectors are very similar, both with polylinkers for the addition of gene-specific...at once. Therefore, applications that require simultaneous modification of all alleles of a gene, or which...which require modifying multiple genes simultaneously in the same cell, are more appropriate for a CRISPR-based...

...fastest test that has been developed so far.  A similar method has been optimized to detect the SARS-CoV...and will not give a positive result for highly similar viruses. Unlike Cas12a, Cas13a does not require...illuminator or UV light illuminator. Approach: Highly similar to the SHERLOCK diagnostic test, this test varies...

...a miniaturized version of Spinach that retains similar levels of fluorescence when bound to DFHBI despite...of artificial guide RNAs that can be used to simultaneously target cellular RNA to specific intracellular... Broccoli are derived from Broccoli, and share similar properties to Broccoli despite binding to a different...

...confrontational situations with others because you simply cannot see things from their point of view and ...herself to the nearby village (and to the reader) by simply listening. Chances are, you know someone like this...how previous iterations of those teams resolved similar issues in the past, or how completely different...

...Magic Pocket Cinema Camera 4K. It’s wonderful and simple to use, but when you need to juggle filming and...perfect height (Fig. 2). We recorded video using a simple, external video recorder and a mic clipped to her...video conferencing software. Interviewees could simply boot up Zoom and record themselves talking to their...

...the immune response. The second strategy is the simple removal of the HIV-1 genome from infected cells...efficiency differed from 48-100% between strains. In a similar experiment, CRISPR reduced the HIV-1 copy number...evidence that an HIV-1 cure may be within our reach. Similar studies have shown that CRISPR can be used to combat...

...postdocs. Unfortunately, even when universities have simple postdoc numbers sorted out there can be gaps in...important take-away from this session was the idea that simply satisfying a percent requirement for diversity ...difficult question but not one that should be abandoned simply because it is difficult and is key to the continued...

...complementary Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP...-Veenboer C, Barker N, Klein AM, van Rheenen J, Simons BD, Clevers H. Cell. 2010 Oct 1;143(1):134-44. ...Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature Methods. 2011 Mar;8(3):253-9. doi: 10.1038...

...cells? Immunoblotting or simply the western blot, or western, is one of the simplest methods to detect the...could contain unintended cross-reactive proteins. Similarly, if you vary the experimental conditions from ...

...the go-to choice for studying mammalian biology. Similar to any model, mice are not without their problems...manipulate the mouse genome using CRISPR/Cas9. Similar to the human genome, the mouse genome is made up... finally, we have a technology, CRISPR, that is simple in concept, straightforward to use, and robust ...

...I changed jobs. I highly recommend taking on a similar position in your field of interest before you dive... solution attempted was more meetings, but this simply seemed to create more bureaucracy. As with any ...anticipated and there can be a glut of individuals with similar skill sets. This is not to say that all commercial...

... cell from the clinic. The 2nd generation is similar to the first one with the exception that the HIV...must be chosen carefully. Often researchers want a simple set-up where the gene of interest is expressed ...experience in transfection, CaCl2 transfection is a simple and cheap technique that should work well. Quickly...

...pitfalls of accepting alternative facts. Because, similar to scientists, the legacy of each government and..., challenged us. I worried the march would be a simple, unquestioning celebration of science, at a time...research scientist would be a career that could simply cease to exist. But then November hit and panic...

...lysing cells and removing cellular debris look very similar. However, keep in mind that kit components (i.e... Figure 2: Plasmid isolation workflows are similar across kits. Lyse your bacteria to release the ...can be more flexible in this situation as you can simply add more beads to your sample. But, you’ll need...

... every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most... change the genomic target of the Cas enzyme by simply changing the target sequence present in the gRNA...The requirements for a CRISPR knockout assay are simple. You’ll need a Cas enzyme and a gRNA specific to...AcrVA3.1, which inhibits both Cas12a and Cas3. Similarly, some Acr proteins are specific to a particular...editors are based on cytosine base editors and similarly work by first converting cytidine to uridine. ...repress gene expression in mice and human cells. The simplest dCas9-based activators and repressors consist ...imaging has numerous advantages, including the simplicity of gRNA design, programmability for different...

...likely to express the target at a robust level, similar to that in samples of interest. If you are working...   If your protein of interest is expressed at similar levels in your cell lines, you can consider a cell...transiently overexpressed in CHO cells and displayed similar labeling patterns on different primary blood leukocytes...

...audience blindly receiving them; it results in highly similar content and the absence of iterative design; it...published 1 Jan 2013, no longer available), is a simple catalogue of reported sgRNA designs. The second...The third, WGE (published 15 Sep 2015), is more similar to EENdb than CrisprGE, but is also a good example...

...a single recombination reaction, drastically simplifying the process and reducing the amount of time required...specific recombination sites (in this regard, not so dissimilar from restriction enzyme cloning). To do this ...selection cassettes). Although gateway cloning is a simple and efficient cloning method, gateway vectors and...benefit of Gibson cloning is that it allows for the simple assembly of multiple fragments of DNA in the chosen...Oligonucleotide Stitching Yeast-mediated cloning is very similar in principle to Gibson cloning, but instead of ...the powerful recombination abilities of yeast. Similar to Gibson, this method can efficiently fuse two...

...protein that enables imaging of two biosensors simultaneously   Researchers in the labs of Michael Lin ...mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring the activities of CaMKII and RhoA...PhoCl between an enzyme and an inhibitor domain similarly allows for control of enzymatic activity. In addition...

... of the best ways to learn about a new field is simply to know where to look and how to start. There is...  About the author Niko McCarty is the editor of Asimov Press and is designing a genetic engineering curriculum...