We narrowed to 303 results for: SPI;

1. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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   Blog Post

   ...only saw incorporation of the blocking mutation. Despite the fact that both the blocking mutation and our...

2. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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   Blog Post

   ...wheat, mushroom, and rice (Khatodia et al., 2016). Despite the almost universal functionality of the CRISPR...

3. Using Phosphoserine to Study Protein Phosphorylation

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   ...organisms lacked a classic cysteinyl tRNA synthetase, despite abundant incorporation of cysteine in their proteomes...

4. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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   Blog Post

   ...strategy may be a better option than direct cleavage. Despite the potential difficulties in translating these...

5. Treating Muscular Dystrophy with CRISPR Gene Editing

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   ...wasting leading to death at around 30 years of age. Despite the amount of research conducted on DMD, there ...

6. Thoughts on the Future of Research 2015 Boston Symposium

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   ...clearly not equitable to that of other trainees despite the fact that the underrepresented individual is...

7. Is this the right place for me? 8 tactics for choosing a lab

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   ...Will you learn how to run a lab if that is your aspiration? Will you help or at least observe grant writing...

8. CRISPR 101: Epigenetics and Editing the Epigenome

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   ...methyltransferase from the prokaryote Mollicutes spiroplasma (M. Sss1) (termed MQ1.) The Q147L mutation improves...

9. Plasmids 101: Repressible Promoters

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   ...to the promoter and turning transcription OFF. Despite their bacterial origins, Tet systems function well...

10. Hot Plasmids: Spring 2025

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    Blog Post

    ...expressing iGluSnFR3, iGluSnFR4f, and iGluSnFR4s. C) Spike-triggered averages for single action potential-evoked...

11. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    ...cardiac, and retinal cells (Issa et al., 2023). AAV2 Despite being isolated second, AAV2 is the best studied...

12. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    ...described in Nielsen et al (3) and Lou et al (4). Despite choosing proper register parts and designs, we ...

13. 9 tips for a successful postdoctoral experience

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    Blog Post

    ...new pieces of data, your talk will probably not inspire you or the audience. Ask yourself: What is the...

14. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    ...these containers. Clean up any media or culture spilled immediately. Water baths, liquid nitrogen containers...

15. Antibodies 101: Validation

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    ...to be present and recognizable by your antibody despite the manipulation. Additionally, not all sample ...

16. When Fidelity Matters: A frank discussion about ligase fidelity

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    ...fidelity") better than T4 DNA Ligase (21, 25, 26). Despite this higher fidelity, Taq DNA Ligase can still ...

17. A History of Genome Engineering in Popular Culture

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    ...advancements and the noteworthy work of fiction that it inspired. CAUTION: Spoilers alert! Pre-DNA Era  H.G. ...

18. Antibodies 101: Antibody Engineering and Directed Evolution

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    ...synthetic antibody products and development methods. Despite recent advances, there is still always room to ...

19. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    Blog Post

    ...Boyden et al., 2005; Deisseroth et al., 2006). Despite its relatively recent development, the history ...

20. Important Considerations When Using AAVs

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    ...to their Biosafety Level-2 or 2+. Please Note: Despite the fact that AAVs themselves are not pathogenic...