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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 718 results for: STO

Showing: 241 - 260 of 718 results

  1. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...sequence of a mCherry fluorescent protein followed by a stop codon and the coding sequence for a green fluorescent...success of the change in expression of GFP, for the stop codon should have changed from “TGA” to “TGG.”  ...acceptor and donor sites, and introducing premature stop codons.    New from the viral service By Jason...

  2. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...to argue otherwise). To be sure, throughout the history of medical research, there have always been people...CRISPR/Cas9 research” but whether or not we will stop shuffling our feet on necessary actions to review...the future of genome editing technology. We must stop punting decisions on scientific, ethical, regulatory...

  3. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...quick turn-around time of Sanger sequencing have historically matched the needs of most molecular biology ...displayed at the individual read level, and because custom primers or "primer-walking" reactions are completely...problems. He received his PhD in Bioinformatics from Boston University, and did postdoctoral work at Harvard...

  4. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...enormous lattice of identical subunits. A brief history of R bodies R bodies have been studied for many...recombinant E. coli on paramecia. European Journal of Protistology 48, 290–296 (2012). PubMed PMID: 22356923. 3... Polka, J. K. & Silver, P. A. A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo...

  6. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...them.   When you think about it, virtually every milestone in a scientist's career is mediated by some kind...flagship workshop.  Our 2018 workshop took place in Boston - you can revisit the activities of our attendees...careers in science communication The strength of story telling Science communication: 9 strategies to get...

  7. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...RNA-editing Cas13 enzymes have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock ...from this paper are available at Addgene. Yan, Winston X., et al. “Cas13d Is a Compact RNA-Targeting Type...Plasmids Check out our CRISPR Guide Read our CRISPR History  ...

  8. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ... addition, there was an entire story-telling session organized by Story Collider and which featured a ...the different parts of the brain stimulated by stories. In retrospect, I should have expected this given...

  9. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...design a custom primer. Because Sanger sequencing can usually sequence just 1 kb of DNA, custom primers...All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab. It contains an ampicillin resistance marker...

  10. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic biology scene in 1989.... While the codon used is often one of the three stop codons ­– most commonly the UAG codon – in some ... V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry, 60(46), 3455–3469...

  11. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...assembled a cross-team team, with members from the Customer Service, Data Curation, Outreach, Product, Scientist...particular and our deep understanding of Addgene’s customer’s needs. Categories We created a categorization...of our information in order to more granularly customize the search engine, and a scoping mechanism that...

  12. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...in C++ and Java Command line history: Tracking command line history by recording all commands entered...addition to output files, this is also where you can store any power point, excel, or other files relevant ...

  13. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...project, I read the book “Every Other Thursday: Stories and Strategies from Successful Women Scientists... group to be intrinsic to their success. Their stories made it clear to me that, executed correctly, the...renowned Healthcare Businesswomen’s Association (HBA) Boston Chapter and the Massachusetts Association for Women...

  14. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...well received, and then when the team started brainstorming ideas for the next workshop, they realized that...to practice and teach reproducibility An early milestone for the group was the “reproducibility for everyone... Schwessinger for helpful conversations on the history of the group, lessons learned, and to Sonali Roy...

  15. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...Segall-Shapiro, Thomas H., Eduardo D. Sontag, and Christopher A. Voigt. "Engineered promoters enable constant...2. Kittleson, Joshua T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization of gene dosage...Synthetic Biology: A Conversation with MIT’s Christopher Voigt Resources on Addgene.org See the synthetic...

  16. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published August... studies, the edited embryos proceeded to the blastocyst stage at similar rates to untreated controls....researchers were able to examine each individual blastomere to determine its genotype. However, this detailed...

  17. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ... by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular Cytology and Van ...our guest bloggers Joachim Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor at ...follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the Section of Molecular ...

  18. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...targeted the following antigens: GFP, RFP,  the histone protein H2A2B, and the actin-binding protein gelsolin...however, compatible with most multi-labeling immunohistochemical protocols where either a directly labeled...RANbodies) as versatile, small, sensitive immunohistochemical reagents. PNAS. PubMed PMID: 29440485. Additional...

  19. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...faculty (at time of writing, Brigham And Women's in Boston also just announced that they were reversing their...does not appear to hold. There are institutions in Boston - Massachusetts General Hospital, Mass Eye and ...that are not raising salaries when all others in Boston (some of them literally across the street from ...

  20. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...six figures. Averaging ten panels each. (For this story, it is important to know that the student presenting... it. When my mind went blank, my options were to stop talking until I could present something coherent... something as simple as telling yourself, “I can stop and check my notes if I need to,” or “It is okay...
Showing: 241 - 260 of 718 results