We narrowed to 578 results for: SUB

1. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

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   ...or antibodies against common tumor markers to subsequently capture cancerous cells expressing low levels...depositing your plasmids with Addgene. Howarth: We submit all our plasmids that may well be of use to others...

2. Sensing Neuronal Dopamine

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   ...dopaminergic neurons in a brain region called the substantia nigra, which is the main source of the body’s...sensor, dLight1.1 and dLight1.2, exhibit high (submicromolar) DA affinity and high-magnitude responses (increases...

3. Bringing Sustainable Practices to the Lab: Recycling

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   ...popularity of single-use items, plastic is a substantial portion of lab waste. Despite the fact that researchers...PMC5517107. Additional resources on the Addgene blog Subscribe to the Addgene blog Find lab tips for your research...

4. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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   ...serves to stabilize DNA-protein interactions for subsequent sheering of DNA by sonification. Though it helps... short DNA fragments can then be purified for subsequent library preparation and high-throughput sequencing...

5. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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   ...determine potential water contaminants and to subsequently develop technologies to protect fresh water ...Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for updates and future collaborations...

6. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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   ...just a fraction of a single genetically-defined subset of cells. By labeling fewer cells in a population... genetic tool that allows for the labeling of a subset of cells with the same genetic makeup, overcomes...

7. Management for Scientists: What Makes a Good Manager Anyway?

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   ...prefer to be approached as peers rather than subordinates Need mental space and dislike intrusions (this...Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the entire series...

8. 10 Ways to Share Your Science!

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   ...reasons to feel obligated too, as experts on the subject brought out in a Kavli Foundation Round Table. ... be a good way to follow news about particular subjects or find out what others are reading. Check out...

9. The Golden Stopwatch Award: Recognizing Facilitators of Research

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   ... deposit agreement must be put in place. And subsequently, every time that material is requested, a material...find some easy talking points for broaching this subject with your institution’s tech transfer professionals...

10. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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    ...Karolinska Institute developed mNeonGreen via 21 substitutions to LanYFP and the addition of the enhanced GFP...choosing fluorescent proteins for your research Subscribe to the Addgene blog Resources on Addgene.org ...

11. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...labeling just a fraction of a genetically-defined subset of cells. By labeling fewer cells in a population...

12. Summer SciComm Series: Master of SciComm

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    ...to what the research had to say. Moreover, this subject allowed me to engage in findings relevant to my...areas in which I knew very little. Diving into subjects like risk perception, scientific uncertainty and...

13. Donations from Addgene to Yield Answers for Rare Disease Researchers

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    ...Accelerate Research Discoveries) Award attracted submissions from 99 universities and foundations in 21 countries...Rare Genomics Institute and Addgene will make a substantial difference in pushing the work forward. Xeroderma...

14. Another Pathway into Cells: iTOP

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    ... be helping the untagged protein enter cells. Subsequent work found that both NaCl and non-detergent sulfobetaine... be used to transduce Cas9 and a gRNA, which subsequently enter the nucleus and cleave the specified target...

15. Visualizing Genomic Loci with CRISPR-Sirius

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    ...hairpins into the gRNA tetraloop and went through subsequent design iterations to increase stability and minimize... used to visualize relative locations of the subtelomeric region of the pericentromeric region (PR1), ...

16. Reagent Repositories Are Speeding up Science During the Pandemic

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    ... could not enter cells expressing murine ACE2 (Subbarao et al., 2006). Now that a similar phenomena is...: . https://doi.org/10.1038/s41467-020-15562-9 Subbarao K, Roberts A (2006) Is there an ideal animal model...

17. Hot Plasmids - January 2023

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    ...Every few months we highlight a subset of the new plasmids, antibodies, and viral preps in the repository... here!   Andrews NP,  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

18. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    ... as a whole or can be used to isolate specific subsets like CD4+ or CD8+ T-cells or monocytes. PBMCs can... lymphatic system or even the human skin and subcutaneous models (Groell et.al, 2018). Treating these ...

19. What Good Citizenship Can Do for Reproducibility in Science

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    ...conditions will derail a research program (think a subtle difference in mouse chow, a different water filtration...did. Admittedly, the differences are often very subtle, and it can take a lot of detective work to figure...

20. R Bodies: Membrane-Rupturing Microscopic Tools

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    ...bodies are a polymer of many copies of monomeric subunits. Unlike cytoskeletal polymers, however, R bodies...propagating through an enormous lattice of identical subunits. A brief history of R bodies R bodies have been...