We narrowed to 581 results for: SUB

1. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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   Blog Post

   ...serves to stabilize DNA-protein interactions for subsequent sheering of DNA by sonification. Though it helps... short DNA fragments can then be purified for subsequent library preparation and high-throughput sequencing...

2. Bringing Sustainable Practices to the Lab: Recycling

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   Blog Post

   ...popularity of single-use items, plastic is a substantial portion of lab waste. Despite the fact that researchers...PMC5517107. Additional resources on the Addgene blog Subscribe to the Addgene blog Find lab tips for your research...

3. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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   Blog Post

   ...determine potential water contaminants and to subsequently develop technologies to protect fresh water ...Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for updates and future collaborations...

4. Management for Scientists: What Makes a Good Manager Anyway?

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   Blog Post

   ...prefer to be approached as peers rather than subordinates Need mental space and dislike intrusions (this...Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the entire series...

5. 10 Ways to Share Your Science!

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   Blog Post

   ...reasons to feel obligated too, as experts on the subject brought out in a Kavli Foundation Round Table. ... be a good way to follow news about particular subjects or find out what others are reading. Check out...

6. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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   Blog Post

   ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...labeling just a fraction of a genetically-defined subset of cells. By labeling fewer cells in a population...

7. The Golden Stopwatch Award: Recognizing Facilitators of Research

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   Blog Post

   ... deposit agreement must be put in place. And subsequently, every time that material is requested, a material...find some easy talking points for broaching this subject with your institution’s tech transfer professionals...

8. Summer SciComm Series: Master of SciComm

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   Blog Post

   ...to what the research had to say. Moreover, this subject allowed me to engage in findings relevant to my...areas in which I knew very little. Diving into subjects like risk perception, scientific uncertainty and...

9. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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   Blog Post

   ...Karolinska Institute developed mNeonGreen via 21 substitutions to LanYFP and the addition of the enhanced GFP...choosing fluorescent proteins for your research Subscribe to the Addgene blog Resources on Addgene.org ...

10. Donations from Addgene to Yield Answers for Rare Disease Researchers

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    Blog Post

    ...Accelerate Research Discoveries) Award attracted submissions from 99 universities and foundations in 21 countries...Rare Genomics Institute and Addgene will make a substantial difference in pushing the work forward. Xeroderma...

11. Another Pathway into Cells: iTOP

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    Blog Post

    ... be helping the untagged protein enter cells. Subsequent work found that both NaCl and non-detergent sulfobetaine... be used to transduce Cas9 and a gRNA, which subsequently enter the nucleus and cleave the specified target...

12. Hot Plasmids - January 2023

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    Blog Post

    ...Every few months we highlight a subset of the new plasmids, antibodies, and viral preps in the repository... here!   Andrews NP,  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

13. Reagent Repositories Are Speeding up Science During the Pandemic

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    Blog Post

    ... could not enter cells expressing murine ACE2 (Subbarao et al., 2006). Now that a similar phenomena is...: . https://doi.org/10.1038/s41467-020-15562-9 Subbarao K, Roberts A (2006) Is there an ideal animal model...

14. What Good Citizenship Can Do for Reproducibility in Science

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    Blog Post

    ...conditions will derail a research program (think a subtle difference in mouse chow, a different water filtration...did. Admittedly, the differences are often very subtle, and it can take a lot of detective work to figure...

15. Visualizing Genomic Loci with CRISPR-Sirius

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    ...hairpins into the gRNA tetraloop and went through subsequent design iterations to increase stability and minimize... used to visualize relative locations of the subtelomeric region of the pericentromeric region (PR1), ...

16. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    Blog Post

    ... as a whole or can be used to isolate specific subsets like CD4+ or CD8+ T-cells or monocytes. PBMCs can... lymphatic system or even the human skin and subcutaneous models (Groell et.al, 2018). Treating these ...

17. R Bodies: Membrane-Rupturing Microscopic Tools

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    Blog Post

    ...bodies are a polymer of many copies of monomeric subunits. Unlike cytoskeletal polymers, however, R bodies...propagating through an enormous lattice of identical subunits. A brief history of R bodies R bodies have been...

18. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    Blog Post

    ...target certain sites, or make only a subset of base substitutions.  The need for speed: base editing ...

19. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    Blog Post

    ...results of all four of those tests and not just the subset of those that return a particular result. Anything...review occurs before results are known. The author submits the research plan (similar to a preregistration...

20. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post

    ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids... Weiss’s group used these reporters to define a subset of active miRNA’s unique to HEK293FT and HeLa cell...