Addgene

We narrowed to 382 results for: Scrib

Showing: 241 - 260 of 382 results

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Multiplexed Overexpression of Regulatory Factors (MORF) collection

Type

Blog Post

...approximately 1,800 TFs, with >3,500 unique isoforms transcribed from these sites. The Zhang lab generated a comprehensive...
Revamp Your Lab Meetings With Creative Virtual Collaboration

Type

Blog Post

...pivotal in writing publications/grants too. As described by several works (Wuchty et al., 2007, Vicens ...
Four Factors that Differentiate the Stem Cell Field

Type

Blog Post

...speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka...
Optimizing Donor DNA for Enhanced CRISPR Genome Editing

Type

Blog Post

...relatively modest, requiring expressed Cas9 protein, transcribed sgRNA, and optional donor DNA. Chris Richardson...
Fluorescent Proteins 101: Luciferase

Type

Blog Post

...emission at 560nm. Shortly after the initial article describing the cloning of firefly luciferase was published...
Multiplex Genome Editing with CRISPR-Cpf1

Type

Blog Post

...editing! A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing. ...
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

Type

Blog Post

...about Addgene in our "Inside Addgene" blog series Subscribe to the Addgene blog Resources on Addgene.org ...
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

Type

Blog Post

... Lab’s recent Nature Biotechnology paper, they describe TurboID and miniTurbo, which utilize non-toxic...
AAV Q&A with Tim Miles

Type

Blog Post

...Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors Blog Posts ...
Viral Vectors 101: Biosensors

Type

Blog Post

.... Physiological factors Unlike the previously described sensors, this class measures either factors as...
GCE4All: Making Genetic Code Expansion Accessible

Type

Blog Post

...through a seminal paper from Peter Schultz’s lab describing a technique that allowed for intentional incorporation...
Viral Vectors 101: The AAV Retrograde Serotype

Type

Blog Post

...trillion connections (synapses.) In this post we’ll describe the AAV retrograde serotype (AAVrg) and how it...
Viral Vectors 101: Gamma-Retroviral Packaging Systems

Type

Blog Post

...lentiviral packaging methods. The three components described above (envelope, packaging, and transfer) are ...
Antibodies 101: Flow Cytometry Controls

Type

Blog Post

...controls like FMO or isotype controls, which we’ll describe a bit later. Single Colors Single color controls...
CasPEDIA: A Functional Classification of Cas Enzymes

Type

Blog Post

...relevant resources The homepage displays a graphic describing CasIDs and relevant properties of Cas enzymes...
Plasmids 101: Blue-white Screening

Type

Blog Post

...process is called α-complementation. The system described above was put to practical use in the following...
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Type

Blog Post

...worries, I've summarized the three strategies described in this post in the graphic below - including ...
CRISPR 101: Non-Homologous End Joining

Type

Blog Post

...better substrates for ligation. Although we do not describe these steps here, the processing of DNA ends tends...
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

Type

Blog Post

...Konermann and her colleagues in Feng Zhang’s lab describe just such a system, which they call CRISPR/Cas9...
Plasmids 101: Yeast Vectors

Type

Blog Post

...differ from the bacterial elements previously described. Why do scientists use yeast vectors? Yeast are...

Showing: 241 - 260 of 382 results

Results per page:

Multiplexed Overexpression of Regulatory Factors (MORF) collection

Revamp Your Lab Meetings With Creative Virtual Collaboration

Four Factors that Differentiate the Stem Cell Field

Optimizing Donor DNA for Enhanced CRISPR Genome Editing

Fluorescent Proteins 101: Luciferase

Multiplex Genome Editing with CRISPR-Cpf1

Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

AAV Q&A with Tim Miles

Viral Vectors 101: Biosensors

GCE4All: Making Genetic Code Expansion Accessible

Viral Vectors 101: The AAV Retrograde Serotype

Viral Vectors 101: Gamma-Retroviral Packaging Systems

Antibodies 101: Flow Cytometry Controls

CasPEDIA: A Functional Classification of Cas Enzymes

Plasmids 101: Blue-white Screening

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

CRISPR 101: Non-Homologous End Joining

Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

Plasmids 101: Yeast Vectors

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