...This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells...Protocols Viral Service Introduction This protocol describes how to generate a monoclonal cell line from a ...type will be able to grow under the conditions described in this protocol. Conditioned medium (details ... specific conditions used. Each 96-well plate described in this protocol could, in theory, give rise to...protocol was performed at Addgene on the A549 cells described in our sample data , each 96-well plate gave rise...from Feng Zhang (Addgene plasmid #52962 ) and is described in Improved vectors and genome-wide libraries ...

...This protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected...Protocol Antibody Guide Introduction This protocol describes how to run an indirect Enzyme-Linked ImmunoSorbent...antigen to validate an antibody. This protocol will describe how to create a standard curve using known concentrations...

...bath on and set the appropriate temperature as described in your protocol. How to set the temperature will...water bath even if you are using disinfectants as described above in step 3. You will also need to maintain...

...This protocol describes the basic steps for fixing and staining cells in culture with a primary antibody...antibodies to detect antigens in cells. Here we describe the basic steps for fixing and labeling cells ...

...This protocol describes how to affinity purify recombinant antibodies from cell culture supernatant using...Western Blot Protocol Introduction This protocol describes how to affinity purify recombinant antibodies ...

...This protocol describes the basic steps for lysing cells, determining total protein concentration in ...specific to the protein of interest. This protocol describes the basic steps for lysing cells, determining ...

...activating pulse and follow all specifications described in the equipment manual. Immediately add 1 mL ...µl aliquots of cell/DNA mixture used (8 in the described protocol) using a new vented falcon tube for every...

... Bacterial Culture Introduction This protocol describes methodology for plating antibiotic over-agar for...

...Protocols Viral Service Introduction This protocol describes a general method for transfecting mammalian cells...

...gene of interest in cell culture. This protocol describes how to transfect suspension HEK293 cells with ...

... several μg/μL. The protocol below is meant to describe the general procedure for purifying plasmid DNA...

...from Feng Zhang (Addgene plasmid #52962 ) and is described in Improved vectors and genome-wide libraries ...

...plasmid of interest. For this example, we will describe how to copy a cDNA from one vector into a new ...

...Purification Protocol Introduction This protocol describes how to determine the purity and concentration ...

... Biology Reference Introduction This protocol describes how to use droplet digital PCR (ddPCR) to titer...

... Biology Reference Introduction This protocol describes how to extract RNA from tissues or cells without...

...Guide Virus Blog Posts Introduction This protocol describes how to use droplet digital PCR (ddPCR) to titer...

...license or rights of access or use to the materials described in the literature. The distribution of materials...