...Salvador family WW domain containing protein 1 SCRIB Scribbled planar cell polarity protein SHC SHC1 SHC2 ...

...pSerOTS-C1* (V70) 188537 ). These reagents are described in detail in (Mohler et al., Mol Syst Biol 2023...Phosphorylated Isomers (iSPI). This new resource described in (Gassaway et al., Nature Methods 2022) is adapted...Iterative Synthetically Phosphorylated Isomers (iSPI) described in: A multi-purpose, regenerable, proteome-scale...in a new window) rEcoli XpS and a new pSerOTS described in: System-wide optimization of an orthogonal ..., 551 KB) Human Serine Phosphopeptide Library described in: Encoding human serine phosphopeptides in bacteria...in a new window) Improved Reagents (ca. 2015) described in: A flexible codon in genomically recoded Escherichia...Reagents (DOCX, 525.9 KB) Reagents (ca. 2014) described in: Enhanced phosphoserine insertion during Escherichia...

...expression plasmids. Large collections or kits are described below and not included in this table. For a more...strains. CRISPR plasmids from John Calarco's lab as described in Heritable genome editing in C. elegans via ...genome-editing events. Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing...also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery of Custom Genetic...C. elegans and other nematodes. Wormbuilder - Describes methods to engineer the genome of the genetic ...

...zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One 2009) pMLM292 (for expression...zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One 2009) pMLM800 (for expression...Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and Cade et al.,...

... references are below. Available plasmids are described below: 61591 : PX601; CMV-driven SaCas9; U6-driven...sgRNA to a constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with...Constitutively active Cas9 mouse Available plasmids are described below: 61408 : Targeting vector for the mouse ... backbone plasmid for cloning new targets are described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA...present in the mouse genome. These plasmids are described here. #60227 - AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR...

...production of genomic deletions. In addition, we describe techniques for deletion, identification, and characterization... the genomic recognition site. NOTE: Figure 1 describes possible deletion strategies for genes and non-coding...deletion. For this approach, the internal primers described in step 2.1 may be omitted. CRISPR Cloning Anneal...plasmids by electroporation 4 . This protocol is described in detail for murine erythroleukemia (MEL) cells...

...that came out in the spring of 2011, the groups describe a set of customized plasmids that can be used ... a TALEN kit through Addgene. This kit, first described in Nature Biotechnology in August, uses a serial...in Nature Biotechnology early in 2011 and they described their kit for assembling TAL effector arrays in...

... is described in more detail in the Multiplex Assays section below. The following table describes the ...of B cells isolated from animals immunized as described above are used to create hybridomas, which produce...primary antibody for your protein of interest, as described in the IP section above. Reverse the crosslink...

...palindromic repeat sequences. (1) The CRISPR array is transcribed to make the pre-CRISPR RNA (pre-crRNA). (2) The...in vitro. In 2013, Cong et al. and Mali et al. described CRISPR-based genome editing in mammalian cell ... of new applications. The first CRISPR papers described two main categories of genome edits. First, the...

...cleaves the target DNA. The Joung lab recently described gRNA and Cas9 expression vectors and validated...Engineering newsgroup here . Links to additional pages describing Joung Lab CRISPR-Cas/RGN reagents: Cpf1 expression...

...regulatory sequences of an RNA polymerase III transcribed U6 snRNA. In both vectors, the target site sequence...non-model invertebrate species. These plasmids are described in: Genome engineering of Drosophila with the ...

...label of their choice according to the methods described by George et al. (2004). The ACP and SFP Synthases...respectively) may be expressed and purified as described previously in Stachelhaus et al. (1998) References...

...recent resurgence of “Do-It-Yourself Science” described in Chapter 3, and of course the rapidly changing... science.”  The three chapters in this section describe the challenges of trusting science in a “fake ... of the book, called simply “Old Science,” he describes the now infamous tale of Rosalind Franklin and...changed significantly over the last decade. He describes the detection of gravitational waves--a story ...collaboration from scientists around the world. The paper describing the waves’ detection had over 1,000 authors. ...This last section of the book, Future Science, describes the author’s thoughts on science’s biggest challenges... doesn’t need to. The problems and challenges described in The State of Science have not changed in “COVID...

... friends on the Cambridge 2010 iGEM team that describes the basics of Gibson assembly as a parody of “...assembly The Gibson assembly technique was first described by Dr. Daniel Gibson and colleagues at the J. ...combined with the popular CRISPR technology and is described in a recent publication by the Lockey lab (Wang...followed by the standard Gibson assembly techniques described above, this resulted in the direct and seamless...using Gibson Assembly along with CRISPR was also described recently (Jiang et al. 2015), in which very large...challenging. Other homology based technologies We've described Sequence and Ligation Independent Cloning (SLIC...homology based assembly methods that have been described--CPEC (circular polymerase extension cloning) ...

...Detect Zika Virus The RNA-based toehold switches described in this post are engineered to bind RNA sequences...Boston University School of Medicine, this post describes a tool you can use to study basic virus biology...researcher in the Silver Lab at Harvard University, describes R-bodies, “force generating protein machines that... Connie Cepko’s Lab at Harvard Medical School describes how AAVs function and indicates important things... postdoctoral researcher in Jacob Corn’s lab, describes how you can better design your donor DNA template...from the Jackson Laboratory for Genomic Medicine described Casilio, a system that uses modular RNA binding...

...plasmids with the original publication where you described them. Search for your article as you would search...purpose of the plasmid. Once you've named and described all of the plasmids in your deposit, you should...process, you will enter plasmid data in the pages described below. You can complete these pages sequentially... Use the "Relevant mutations/deletions" box to describe any mutations within your insert using standard...lowers biosensor affinity for calcium". Please describe any tags or fusions (e.g. His, FLAG, EGFP, etc...the page will open a new set of entry fields to describe the next insert. Repeat as needed for more inserts...Addgene plasmid #52961)".   The vector type should describe how your plasmid is intended to be used. For instance...

...minigenome is capable of being replicated and transcribed in the cell when the appropriate viral proteins...EBOV polymerase replicates the viral genome and transcribes viral genes. Four viral proteins are required...minigenome. These genes and the minigenome are transcribed (and the viral mRNAs are translated) by cellular...polymerase will use the minigenome as a template to transcribe the reporter gene (e.g. firefly luciferase). ...minigenome system Minigenome systems, such as the one described here, are extremely useful research tools. In ...polymerase also needs to be present in the cells to transcribe the viral mRNAs and minigenome. This can be accomplished...

...SSC-H, or FSC-W/SSC-W, respectively. H, A, and W describe the shape of a histogram, which graphically represents... of the cell’s illumination (see Figure 2). H describes the maximum signal strength, while W results from...photocurrent vs. time. The resulting histograms is described through maximum current (H), the time the cell...depends on cell type and laser voltage. The voltage describes an electric potential that can be applied to the...comparing two markers simultaneously, we commonly describe the resulting populations as double positive (...that there are three parameters — H, W, and A — describing the intensity and duration of the detected signal...

...the transcribed gene and its 5’ UTR. Various self-cleaving hammerhead ribozyme parts are described in ...of an RSM is defined by the sequence within a prescribed region of DNA termed the “register”. Each chemical...registers within a sample using a metric that we describe in the Materials and Methods section of our paper...to read states by qPCR or Sanger sequencing as described in the Material and methods section of our paper... Multiple large serine recombinases have been described in the literature (5, 6) and may be substituted...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...For more information, see the bioRxiv preprint describing ICE . Deep Sequencing Data Analysis CRISPResso...