...plasmids get their name? In 1952, Joshua Lederberg set out to clarify the classification of these cytoplasmic...

... lab members as possible. Try to get them in a setting out of the lab (coffee, lunch or beer) and in 1...

...Estimate of time required: ~3 h Workflow Timeline Plate set-up: 2 h qPCR run: 1.5 h Data analysis: 30 min Equipment...load the standards and samples onto the qPCR plate Set up and load the 96-well plate: Load 5 μL of each ...repeat 39x from step 3 / melt curve Example of plate set-up: 1 2 3 4 5 6 7 8 A 1.00 x 10 9 1.00 x 10 8 1.00...

...spectrophotometer 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead bath Clamp stand and clamps... NanoDrop Spectrophotometer using the A280 IgG setting. Combine all eluates with measurable protein. Determine... NanoDrop Spectrophotometer using the A280 IgG setting. If the concentration of the pooled sample is above...

... LDR has also been extended to multiplexed probe sets that allow the simultaneous interrogation of multiple...SNP sites (15). The related LCR technique uses two sets of probes to achieve exponential amplification. ...

... the experimental setup and objectives. Most systems can only excite and read a set number of excitation...this is limited by the filter cubes and filtering settings of the equipment. Systematic evaluation of commercially...

...other epitopes and scaffolds to build a more robust set of frankenbodies. References Ewert, S., Honegger...

...first 3 books of a larger epic of fantasy novels. Set in a world where 1000 years ago the hero lost and...

...break. The Xiao Wang Lab has recently deposited a set of plasmids that helps identify base edited cell ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...compact Cas13 proteins found in the metagenome datasets from hypersaline samples, were engineered and ...

...percentage of the recombined vector backbone.   Ready, set, grow! Our 5 Tips for Troubleshooting Viral Transductions...

...establish that schedule. There’s also tips for setting writing goals, tracking progress, and starting ...

...long email. If you have more to say, email again or set up a meeting. If you really got along well, suggest...

... most of these exploratory conversations. If you set yourself a goal of doing at least two informational...

...beneficial for a low number of cells and in vivo settings. Find the sgRNAiBAR library!  Zhu et al., Genome...remove a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids... a high multiplicity of infection, because this setup allows for experimental replicates for each of the...labeling just a fraction of a genetically-defined subset of cells. By labeling fewer cells in a population...

...Single-Stranded Adeno-Associated Viral Genomes in Academic Settings. Human Gene Therapy Methods 30:195–205 . https...vector genomes as containing just the expression cassette that we want, it also includes a small amount ...

...Preventable errors in the design of cloning strategies set experiments back days or even weeks. Primer design...

...majoring in biochemistry in college and play the drum set as their personal instrument. They are the oldest...options from the Addgene catalog. These were a miR30 cassette, Syntaxin3, and osa-miR528. My manager, Katherine...

... as to how the shared reagents should be used. Setting clear expectations from the beginning makes the...

...easily achieved with alternative methods. Ready, set, transduce! Making virus can sound daunting, but ...

... no idea where to start. Thankfully, my managers set aside time for me to complete an online social media...