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Addgene
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  1. Addgene: The First Twenty Years

    Type
    Blog Post
    ...scientists. She, Kenneth Fan, and Benjie Chen decided to start an open science nonprofit that would facilitate ...Excellence Award for our electronic MTA system. We also started our blog and YouTube channel in 2013, which continue...

  2. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...some require going back two steps, some require starting over entirely, and our software needs to handle... tricky procedure failed and one of your techs started over with a new tube. In the first example we’...

  5. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...yeast), S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus (rabbit), synthetic, or other...Like to Find at Addgene Resources on Addgene.org Start Your Deposit Deposit FAQ Which Plasmids Should I...

  6. Engaging with science and society at pgEd

    Type
    Blog Post
    ...School, has a mission of raising awareness and starting conversations about both the benefits as well ...submitting op-eds to your local newspapers, or starting your own blog. Even if you do not end up pursuing...

  9. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...transcriptional units, and multigene constructs. Let’s start by examining individual parts and transcriptional...MoClo Interested in using MoClo? You don’t have to start from scratch! As you can imagine, there is an array...

  13. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...considered "Cre-On" meaning that your gene of interest starts in the inverse/antisense "off" position and is ...employed for "Cre-off" (i.e. your gene of interest starts in the sense orientation and Cre is used to flip...

  14. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...101 eBook to get all the background you need to start working with plasmids! What are termination and ...Addgene.org Browse our Plasmid Reference Pages Just Starting Work in a New Field? Check out Our Plasmid Collections...

  15. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...directed to a target upstream of the transcriptional start site (TSS) of a gene of interest, it induces potent...directed to a site downstream of the transcriptional start site of a gene of interest, Cas9 forms a steric ...

  18. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...relatively quick. After transfection, HEK-293T cells start producing virus within 24 to 48 hours. Harvesting...can even produce a P3 virus! Time Sf9 cells will start producing virus within a day or two, but unlike ...

  19. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...of common E. coli strains. Increase the volume Starting with a larger culture volume can improve plasmid...serve to increase cell density and plasmid yield. Start with a single, fresh colony Sub-culturing directly...

  20. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...in the example above that the bread crumbs trail starts in the browse page, continues through the depositing...the plasmid in your experiments: Backbone: The starting vector that the depositing lab modified to create...
Showing: 241 - 260 of 562 results