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Showing: 1 - 20 of 29 results

  1. AAV for Neuronal Tracing

    Type
    Collection
    ...is provided to the starter cell in trans , via delivery by an AAV. Since this starter cell now has all ...TVA and G-protein is delivered to the starter cell. (2) The starter cell now expresses TVA and G-protein... RAVB is delivered to the starter cell, and specifically targets the starter cell via the EnvA receptor...). Thus, G-deleted rabies can infect a single "starter" cell, but new virus cannot spread to any neighboring...can spread to cells that are projecting to the starter cell). Once in the secondarily infected cells that... the targeting of G-deleted rabies to specific starter cells, the tropism of this virus was modified so...receptor. RABV is able to replicate in the starter cell using the G-protein, which is supplied in trans . (...

  2. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ... the start of my target sequence? The human U6 promoter prefers a 'G' at the transcription start site ...when they order the oligo. If your spacer sequence starts with a 'G', you naturally have one and do not need...with that mutation be selected/screened? Before starting your experiment, consider co-transfecting with...

  3. Plan Your Experiment

    Type
    Collection
    ...Expression System Design gRNA Publications Get Started CRISPR is a powerful system that enables researchers...section will provide a general framework to get you started using CRISPR in your research. Although we will...understanding of your experimental goal, you are ready to start navigating the different reagents that are available...necessary for Cas9 to bind target DNA. As such, one can start by identifying all PAM sequences within the genetic...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ...false positives with the original STARR-seq method. Learn more about STARR-seq in our 27 Hot Plasmids from...luciferase. Transcriptional Element Screening Optimized STARR-seq ( S elf- T ranscribing A ctive R egulatory R...reporter. These plasmids improve over the original STARR-seq plasmids by using the bacterial ORI as the core...

  5. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...Alternate Prefix: GAATTC GCGGCCGC T TCTAGA TG (contains start) Prefix Enzymes: EcoR1 , NotI , XbaI Suffix: T ACTAGT...Features: Scar encodes Gly-Ser in-frame with prefix start codon; Employs high-efficiency enzymes. Prohibited... Pstl Scar: ACCGGC Features: Modified RFC 10 w/ start and stop codons; Creates in-frame fusions w/ linker...

  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's Michael J Fox Foundation MJFF 40386 pBACgus_Sumostar/LRRK2 1867-2186 LRRK2 His, TEV polH Parkinson's...Parkinson's Michael J Fox Foundation MJFF 40387 pBACgus_Sumostar/LRRK2 1867-2186_G2019S LRRK2 His, TEV polH...Parkinson's Michael J Fox Foundation MJFF 40388 pBACgus_Sumostar/LRRK2 1867-2186_C2024S/C2025S LRRK2 His, TEV...Parkinson's Michael J Fox Foundation MJFF 40389 pBACgus_Sumostar/LRRK2 1867-2186_T2031E LRRK2 His, TEV polH...Parkinson's Michael J Fox Foundation MJFF 40390 pBACgus_Sumostar/LRRK2 1867-2141 LRRK2 His, TEV polH Parkinson's...Parkinson's Michael J Fox Foundation MJFF 40391 pBACgus_Sumostar/LRRK2 1867-2161 LRRK2 His, TEV polH Parkinson's...Parkinson's Michael J Fox Foundation MJFF 40392 pBACgus_Sumostar/LRRK2 1867-2176 LRRK2 His, TEV polH Parkinson's...

  7. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...expectations to the best of our knowledge from the start. However, we know there’s a chance that things may...general estimated turnaround time above. After starting viral production, if we determine that we will...

  8. AAV Packaged on Request

    Type
    Collection
    ...By using AAV Packaged on Request, scientists can start experiments as soon as they receive their AAV prep...depend on your location. Share Your Results Once you start getting results from your AAV experiments, remember...

  9. CRISPR Guide

    Type
    Collection
    ...various applications of CRISPR, and help you get started using CRISPR in your own research. CRISPR Basics...Overview of CRISPRi/a Targeting dCas9 to transcription start sites is sufficient to repress transcription by ...recruits Cas3. Cas3 nicks the non-targeting strand and starts degrading the single-stranded DNA, unraveling DNA...

  11. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...homology to the crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require that each...

  12. Plasmid Collections

    Type
    Collection
    ...plasmids and science information you need before starting your experiment. Genome Engineering Genome Engineering...

  13. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...to add to the Addgene collection? Click here to start the deposit process and have your plasmids added...

  14. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Cas3. After nicking the non-targeted strand, Cas3 starts unraveling DNA using its helicase properties, cleaving...

  15. DNA Service - Cloning Grade DNA

    Type
    Collection
    ... hope to aid scientists who want to immediately start cloning upon arrival of their plasmid from Addgene...

  19. Synthetic Biology - Overview

    Type
    Collection
    ...community. To deposit, view our SynBio Deposit Guide or start our online deposit process . Browse SynBio Plasmids...

  20. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...homology to the crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require that each...
Showing: 1 - 20 of 29 results