We narrowed to 614 results for: TIL

... to modify chromatin. A fluorescent protein can still be included for visualization purposes, but the ...gRNA complex binds the target DNA without cleavage until it's activated by light. Once activated, Cas9 cleavage...

...proteins. Discovery of anti-CRISPR proteins That is, until the discovery of anti-CRISPR proteins in 2012 (Bondy-Denomy... RNPs, AcrIIA4 reduced off-target editing while still allowing on-target editing (Shin et al., 2017). ...

...make sure yours counts. Dress professionally but still like yourself. It’s better to be overdressed rather...challenges, your interviewers might.  You’ll probably still be required to give a job talk for a remote interview...

...to both people and the tools we use. Scientists utilize an array of tools that rely on DNA amplification...reference is given, de novo assembly in Geneious can still provide additional insights to resolve the assembly...

...in neuroscience, this architecture is frequently still an open question. The connections between neurons...new neurons came from nearby neurons. It wasn’t until later time points that longer-range connections ...

...is a modified version of L440. This vector is versatile in that it can be used for both in situ hybridization...feedings. Some of the phenotypes are not evident until after amputation. For example, the dramatic “two-headed...

...include embryonic lethality, a short lifespan, or infertility and can arise due to copy number, expression ...would get both mutations onto the same chromosome. Until crossing over occurs between the two loci, F2 and...

...large tags into a precise location in the genome is still inefficient, particularly in human cell lines. Furthermore...large regions of homology enable us to effectively utilize the host cell’s inherent homology directed repair...

...interaction - one that gives our customers the most utility in the least amount of time. Though I now spend...customer support scientists wear many hats at this still relatively small nonprofit. If you do go longer ...

...move in the right direction, the increase notably still does not meet the suggested postdoc salary ($50,...advocating for change: from developing a strategy to distilling a message for the appropriate audience and communicating...

...harsher stripping means more sample loss. If you are still optimizing your system, start with a mild stripping...always be added fresh to solutions. It is also volatile and toxic (and smells bad!), so be sure to use...

...inversion, translocation or cassette exchange. By utilizing recombinases to manipulate genomes, scientists...demonstrating low sensitivity to ambient light, and versatile as it can be activated by both low and high-intensity...

...Figure adapted from Kleinstiver et al., 2016. Until recently, two major elements of Cpf1 characterization...off-target editing in a cell culture model. What we still don't know about Cpf1 In their original paper characterizing...

...paternal grandmothers who experienced famine while still in their mother's womb passed on a trait to their...time.  This challenge was difficult to overcome until now. Alice Ting’s lab has developed a new, plasmid-based...

... related to the actual data collection task but still be beneficial to both.  Coming up with creative ...with the company so they know I’m excited while still being able to take my vacation? I want to learn ...

...the use of AdV for in vivo CRISPR-Cas delivery utilized the Non Homologous End Joining (NHEJ) DNA repair..., 2014). An alternative approach to NHEJ is to utilize the Homology Directed Repair (HDR) to knock in ...

... thousands of genes with minimal effort. The versatility of Cas effectors allows for a wide variety of...AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost entirely...

...wealth of potential, and while many applications are still yet to be demonstrated (or invented), its behavior...results early and often, including works-in-progress still in need of further study and development like MagLOV...

...predominantly fluoresce one color but slowly mature until they predominantly fluoresce a second color. This...multiple systems Terskikh et al. showed that their versatile timer could be used in vitro, in mammalian cells...

...Traditional Cas9 regulators In addition to its utility in making targeted modifications to DNA, Cas9 can.... When complexed with a truncated gRNA, Cas9 is still able to bind DNA but is no longer competent to cut...