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We narrowed to 619 results for: TIL

Showing: 241 - 260 of 619 results
  1. Tips for Technical Support Calls

    Type
    Blog Post
    ...interaction - one that gives our customers the most utility in the least amount of time. Though I now spend...customer support scientists wear many hats at this still relatively small nonprofit. If you do go longer ...
  2. Cpf1 Update: Comparison to Cas9 and NgAgo

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    Blog Post
    ...Figure adapted from Kleinstiver et al., 2016. Until recently, two major elements of Cpf1 characterization...off-target editing in a cell culture model. What we still don't know about Cpf1 In their original paper characterizing...
  3. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post
    ...large tags into a precise location in the genome is still inefficient, particularly in human cell lines. Furthermore...large regions of homology enable us to effectively utilize the host cell’s inherent homology directed repair...
  4. A Look at Addgene's QC Process

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    Blog Post
    ...to both people and the tools we use. Scientists utilize an array of tools that rely on DNA amplification...reference is given, de novo assembly in Geneious can still provide additional insights to resolve the assembly...
  5. Rabies and Neuronal Tracing

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    Blog Post
    ...in neuroscience, this architecture is frequently still an open question. The connections between neurons...new neurons came from nearby neurons. It wasn’t until later time points that longer-range connections ...
  6. How to Prepare for an Industry Interview

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    Blog Post
    ...make sure yours counts. Dress professionally but still like yourself. It’s better to be overdressed rather...challenges, your interviewers might.  You’ll probably still be required to give a job talk for a remote interview...
  7. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...include embryonic lethality, a short lifespan, or infertility and can arise due to copy number, expression ...would get both mutations onto the same chromosome. Until crossing over occurs between the two loci, F2 and...
  8. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ... to modify chromatin. A fluorescent protein can still be included for visualization purposes, but the ...gRNA complex binds the target DNA without cleavage until it's activated by light. Once activated, Cas9 cleavage...
  9. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...inversion, translocation or cassette exchange. By utilizing recombinases to manipulate genomes, scientists...demonstrating low sensitivity to ambient light, and versatile as it can be activated by both low and high-intensity...
  10. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post
    ...harsher stripping means more sample loss. If you are still optimizing your system, start with a mild stripping...always be added fresh to solutions. It is also volatile and toxic (and smells bad!), so be sure to use...
  11. Illuminating Epigenetics with A FRET Based Biosensor

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    Blog Post
    ...paternal grandmothers who experienced famine while still in their mother's womb passed on a trait to their...time.  This challenge was difficult to overcome until now. Alice Ting’s lab has developed a new, plasmid-based...
  12. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post
    ...(Figure 4). When recombination occurs, the gene still functions normally because one of its exons has ...are located in intronic regions, this gene will still be expressed. You'd first screen for this specific...
  13. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post
    ...expression of the gene it follows. For increased versatility, it would be advantageous to adapt PITCh to insert...-independent knock-ins, but this possibility is still being explored. More broadly, Sakuma et al.’s use...
  14. 3D Printing Meets CRISPR Cas9

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    Blog Post
    ...impressive, full-color images of proteins. But they are still flat. To truly appreciate the 3D structure of these... will understand the power of a physical model. Until then, you can only wonder what I am talking about...
  15. Fluorescent Proteins 101: Fluorescent Protein Timers

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    Blog Post
    ...predominantly fluoresce one color but slowly mature until they predominantly fluoresce a second color. This...multiple systems Terskikh et al. showed that their versatile timer could be used in vitro, in mammalian cells...
  16. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ... thousands of genes with minimal effort. The versatility of Cas effectors allows for a wide variety of...AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost entirely...
Showing: 241 - 260 of 619 results