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  1. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post
    ...environment” with a strong focus on publishing, but still made it so she could “get exposure to the drug discovery...and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource Pages  Resources at Addgene...

  2. 5 Steps to Writing Grant Proposals for Grad Students

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    Blog Post
    ...administration information. Carefully read over all parts until all aspects are understood. While reading the funding...research project grant application" by the NIH Still want more information on the application process...

  3. Rabies and Neuronal Tracing

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    Blog Post
    ...in neuroscience, this architecture is frequently still an open question. The connections between neurons...new neurons came from nearby neurons. It wasn’t until later time points that longer-range connections ...

  5. A Look at Addgene's QC Process

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    Blog Post
    ...to both people and the tools we use. Scientists utilize an array of tools that rely on DNA amplification...reference is given, de novo assembly in Geneious can still provide additional insights to resolve the assembly...

  6. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post
    ...harsher stripping means more sample loss. If you are still optimizing your system, start with a mild stripping...always be added fresh to solutions. It is also volatile and toxic (and smells bad!), so be sure to use...

  8. How to Prepare for an Industry Interview

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    Blog Post
    ...make sure yours counts. Dress professionally but still like yourself. It’s better to be overdressed rather...challenges, your interviewers might.  You’ll probably still be required to give a job talk for a remote interview...

  9. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    Blog Post
    ...include embryonic lethality, a short lifespan, or infertility and can arise due to copy number, expression ...would get both mutations onto the same chromosome. Until crossing over occurs between the two loci, F2 and...

  11. Hot Plasmids and Viral Preps - March 2021

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    Blog Post
    ... to modify chromatin. A fluorescent protein can still be included for visualization purposes, but the ...gRNA complex binds the target DNA without cleavage until it's activated by light. Once activated, Cas9 cleavage...

  12. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...inversion, translocation or cassette exchange. By utilizing recombinases to manipulate genomes, scientists...demonstrating low sensitivity to ambient light, and versatile as it can be activated by both low and high-intensity...

  13. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post
    ...large tags into a precise location in the genome is still inefficient, particularly in human cell lines. Furthermore...large regions of homology enable us to effectively utilize the host cell’s inherent homology directed repair...

  14. Tips for Technical Support Calls

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    Blog Post
    ...interaction - one that gives our customers the most utility in the least amount of time. Though I now spend...customer support scientists wear many hats at this still relatively small nonprofit. If you do go longer ...

  16. Cpf1 Update: Comparison to Cas9 and NgAgo

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    Blog Post
    ...Figure adapted from Kleinstiver et al., 2016. Until recently, two major elements of Cpf1 characterization...off-target editing in a cell culture model. What we still don't know about Cpf1 In their original paper characterizing...

  17. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...paternal grandmothers who experienced famine while still in their mother's womb passed on a trait to their...time.  This challenge was difficult to overcome until now. Alice Ting’s lab has developed a new, plasmid-based...

  18. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ... thousands of genes with minimal effort. The versatility of Cas effectors allows for a wide variety of...AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost entirely...

  19. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...wealth of potential, and while many applications are still yet to be demonstrated (or invented), its behavior...results early and often, including works-in-progress still in need of further study and development like MagLOV...
Showing: 241 - 260 of 607 results