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We narrowed to 797 results for: Tec

Showing: 241 - 260 of 797 results

  1. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...genome engineering is done with recombineering, a technique that utilizes phage recombination machinery to...spacer sequence, and these rare events are easily detectable using PCR. The system also functions in S. pneumoniae...MAGE (Multiplex Automated Genome Engineering) technology (Ronda et al., 2016). pMA7CR_2.0 expresses lambda...Scientists have begun to realize the potential CRISPR technology can have on the human microbiome. You can consider...produce sequence-specific antimicrobials. Nat Biotechnol 32:1146–1150 . https://doi.org/10.1038/nbt.3043...efficiently delivered RNA-guided nucleases. Nat Biotechnol 32:1141–1145 . https://doi.org/10.1038/nbt.3011...bacterial genomes using CRISPR-Cas systems. Nat Biotechnol 31:233–239 . https://doi.org/10.1038/nbt.2508...

  2. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...address these limitations, labs have developed techniques like CREATE (Deverman et al., 2016), M-CREATE... on reagents and protocols, visit https://clover.caltech.edu. Figure adapted from Challis et al. 2022....deposited by the Gradinaru lab. You can visit our Caltech Systemic Capsids page to learn more about that ...It shows lower expression in the liver and no detectable transduction in the CNS. In the ENS, it has a...need more options) we recommend you check out Caltech’s CLOVER Center’s extensive repository of published... Gradinaru V, Fox AS. (2023). Advances in AAV technology for delivering genetically encoded cargo to the...throughout the non-human primate brain. Nature Nanotechnology, 1–11. https://doi.org/10.1038/s41565-023-01419...

  3. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...CRISPR and epigenetics As with many TALEN-based technologies, the advent of CRISPR has made targeting much...modification is certainly not the only CRISPR-based technology designed to alter gene expression. Fusing dCas9... genes from promoters and enhancers. Nature Biotechnology 33:510–517 . https://doi.org/10.1038/nbt.3199...regulatory elements in the human genome. Nature Biotechnology 35:561–568 . https://doi.org/10.1038/nbt.3853...programmable TALE-TET1 fusion proteins. Nature Biotechnology 31:1137–1142 . https://doi.org/10.1038/nbt.2726...modifications at endogenous enhancers. Nature Biotechnology 31:1133–1136 . https://doi.org/10.1038/nbt.2701...

  4. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...apply someone else's new and incredibly useful technique simply by following the abbreviated methods section...They also have plans to expand to other protein technologies later. Organizations such as BenchSci and FPbase...fluorescent proteins so you can best apply these technologies to your own research needs. Other companies ...make it much easier to learn new experimental techniques. Electronic lab notebooks also play a role here...larger datasets and use computer based analysis techniques, an important component of reproducibility is...series of blog posts on the defunct genome editing technology NgAgo). In addition, there are organizations ...

  5. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...excellent Technical knowledge and creativity Become a “thought leader,” develop a technical “niche,” develop... something (a journal, a science lunch club, a biotech club) Be the head of a group (like a grad student... develop a useful new assay, master a new technique and be able to talk and own these accomplishments....committee) Follow industry publications like FierceBiotech to “learn the business” Attend events with local...

  6. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...come up. An MTA can resolve those questions! MTAs protect you and your institution, and almost all universities... and adopted by the Association of University Technology Managers (AUTM). This MTA provides many benefits...rights – and the rights of your institution – are protected while we handle all the paperwork. Record ...Addgene is internationally recognized as being protective of both the providing institution and the receiving...receiving institution. Therefore, many technology transfer officers are familiar with the UBMTA and are...

  7. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...engineering company, Genentech, was founded.  This commercialization of gene editing technology led to the development... Diego. The revolution in genetic engineering techniques is a speculation of yesteryear which has been...double-strand breaks (DSBs) in the genome. These technologies rely on the cellular DNA repair machinery, which...genetic engineering and open advertisement of the technology on various non-scientific platforms, CRISPR has...perspective of the current. Although genome editing technologies have brought a renaissance in the field of health...

  8. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...use. In order for this technology to survive the diverse demands of the biotech field, let's look at how...survival tactics of prokaryotes to deliver the technological biological blockbuster known as the CRISPR (...various innovative biological and translative technologies. Addgene has empowered researchers to harness... idea or application that involves CRISPR/Cas technology? Let us know.  References Ishino Y, Hideo,...

  9. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...in government, evidence to inform policies, a protected place to work, funding for science, and numerous...cannot prosper in a world where our medicines, technologies, defense, and governing policies are developed...Nuclear Science and Engineering at Rensselaer Polytechnic Institute. He also studies energy markets in ...water. Many marchers held signs demanding the protection of our Great Lakes and our planet. And as we ...the truth about our world. Jonathan Aguilar (@AggleDetector on Twitter) is a graduate student at Johns Hopkins...being taught to communicate in a very specific, technical manner, so much so that we often find it difficult...

  10. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...photoactivation - and have enabled sophisticated imaging techniques.     Irreversible photoactivation Irreversible...super resolution imaging, where the power of the technique relies heavily on the same fluorophore being imaged... and electron microscopy (CLEM) is an imaging technique that combines electron microscopy (EM) with fluorescence...fluorescence localization data. However, this technique requires harsh fixation conditions to preserve...fluorescent protein induced by blue light." Nature biotechnology 24.4 (2006): 461-465. PubMed PMID:16550175. ... dual color fluorescence nanoscopy." Nature biotechnology 26.9 (2008): 1035-1040. PubMed PMID:18724362...

  11. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...eukaryotes! The technology has been used to optimize protein secretion and expression in the biotech workhorse...once you are up to speed on the basics of the technology. In this blog, we will highlight some common ...applications and kits. Keep in mind that this technology is useful for a variety of purposes beyond what...Heterologous Proteins in Yeast Mitochondria.” BMC Biotechnology, vol. 17, no. 1, Springer Science and Business... Enables Rapid Assessment of Emerging CRISPR Technologies. Cold Spring Harbor Laboratory, 27 Oct. 2023...

  12. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...tools by making their own fluorescent protein technologies widely available to the research community. ... a key advantage over other inducible enzyme technologies. She hopes that other researchers will be able... HMOs. Current HPLC-MS and fluorophore-based technologies are too time consuming in the first case and... two biosensors that could quickly and reliably detect HMOs. Fatima’s first design is a whole cell biosensor...Fatima specifically proposes using this sensor to detect HMOs found on cellular surfaces. After incubating...

  13. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...Biotechnology is no longer just for those working in academia or industry. At BosLab, we identify ourselves...towards enabling everyone to participate in the biotech revolution. Our lab exists in what appears to be...Because we’re conveniently located next to a major Biotech hub, building up our lab was easy. Most of our ...engineer yeast to glow, produce synthetic scents, detect compounds in the environment, and openly publish...Kaczmarczyk is a co-organizer at BosLab, a community biotech laboratory in Somerville MA. Follow her on twitter...

  14. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...Silva, Universidad de Ingeniería y Tecnología (UTEC), Peru Julio Ernesto Valdivia Silva is a master of interesting...identifying new drug targets. An early and accurate detection of PZA-resistance is necessary to improve tuberculosis...engineering: glowing bacteria and a fish robot to detect water pollution Dr. Julio Ernesto Valdivia Silva...synthetic biology resources and microfabrication technologies to generate a self renewable sensor, a bacterium...

  15. CRISPR 101: Cytosine Transversion Editors

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    Blog Post
    ...experimented with adding different proteins to the CGBE architecture. They conducted a CRISPRi screen to identify...forms a chemical crosslink with DNA abasic sites, protecting them from cellular processes that would cause...target-library analysis, and machine learning. Nature Biotechnology, 39(11), 1414–1425. https://doi.org/10.1038/...optimization and ancestral reconstruction. Nature Biotechnology, 36(9), 843–846. https://doi.org/10.1038/nbt...targeted DNA transversions in human cells. Nature Biotechnology, 39(1), 41–46. https://doi.org/10.1038/s41587...enable C-to-A and C-to-G base changes. Nature Biotechnology, 39(1), 35–40. https://doi.org/10.1038/s41587...

  16. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...it’s true that adding histones and chromatin architecture to every diagram portraying some aspect of eukaryotic...when I recently attended the 2018 Chromosome Architecture and Chromosome Organization Keystone Symposium...posters I saw at the 2018 Keystone Chromosome Architecture and Chromosome Organization. These aren’t the...Crabtree GR Cell. 1998 Nov 25. 95(5):625-36. An architectural role for a nuclear noncoding RNA: NEAT1 RNA ...epigenome. If you have been considering some new techniques, the posts below may steer you in the right direction...

  17. Hot Plasmids - February 2022

    Type
    Blog Post
    ... cellular targets Optimized genetic screening technique using Cas12a for multiplexed gene perturbation...s41467-019-10846-1.   Optimized genetic screening technique using Cas12a for multiplexed gene perturbation... To validate this optimized genetic screening technique, researchers identified a synthetic lethal interaction...future genetic screens.   DeWeirdt PC, et al. Nat Biotechnol. 2021. doi: 10.1038/s41587-020-0600-6. The Penn...tags, are optimized for SDS-PAGE gels and can be detected on Western blots via engineered immunoglobulin...

  18. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...quite impressed by how neuroscientists are using techniques from across different disciplines to better understand...many opportunities to learn about and develop technologies that will have impacts on many different facets...ready to discuss their VR helmets, gait tracking technologies, and brain monitors. Walking around the poster... addition to the researcher using sequencing technologies to study microbiome interactions with Alzheimer's... or maybe your favorite FRET-based biosensing technique can be applied to an important neuronal signalling...

  19. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...developing assays.  Protocol architecture The basic (very basic!) architecture of the indirect approach is...direct approach isn’t giving you enough signal to detect your protein of interest, it may be time to look...occasion, usually after other signal amplification techniques have been ruled out.  Ease of access Signal amplification...course, this is much easier said than done! Each technique, and sometimes each individual assay, will need...

  20. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...and success. Customer and technical support Our customer and technical service brands continue to ... We score ~99.9% helpful and the customer and technical service teams put 100% effort into helping our...also lauded by another set of stakeholders - the tech transfer community. In a recent law review article...our image in the world. Addgene is now as much a tech company as it is a reagent company. I used to have...
Showing: 241 - 260 of 797 results