We narrowed to 752 results for: Uty

...there are benefits to optimized libraries that utilize only a few sgRNAs per gene. These libraries provide...., 2016, Sanson et al., 2018). These libraries utilize optimized sgRNAs that were designed based on the...while bypassing these issues. CRISPRi libraries utilize an engineered Cas9, which contains multiple point...transcription factors. The Root and Doench labs utilized tracrRNAs with two PP7 stem loops to recruit additional...

...be easy to get caught up in the minutia of the workday, but that minutia gets much more manageable when...games. In previous years, this event was held at Executive Director Joanne Kamen’s home- a testament to the... the lab was such a master drywall hanger?  4. Utilize your office space too Balance point number 3 with...

...molecules. A solution of a longer DNA fragment will contain fewer molecules than a solution of a shorter...written by Alfonso Timoneda, with significant contributions from Robert Hurt and Mohamed Soufi. So you’ve...(and therefore positive colonies) after each consecutive assembly and transformation round, due to the...

...Liu lab recently used phage-assisted continuous evolution and protein engineering to generate seven new ...J.L., Pandey, S., et al. (2023). Phage-assisted evolution and protein engineering yield compact, efficient...lab recently made two deposits that increase the utility of this technique.  By engineering a light-sensitive...antibodies in Addgene’s collection since we began distribution and has been verified to perform well in standard...

...the lab based team as part of their general job duties. Bigger companies have dedicated technical support...customers, internal resources and databases are scrutinized, and this information is enriched with your technical...discussions can often lead to otherwise unobtainable solutions. To some extent there is the need (or opportunity...problems are the most rewarding parts of the job. Solutions may also be achieved by simply replacing the product...

...antibodies. The most famous ones are influenza hemagglutinin (HA), myelocytomatosis viral oncogene (myc),...labels than fusions to 3 or more HA tags. 4. High-resolutions microscopy As indicated above, smFPs can be used... technologies such as array tomography, super-resolution STORM imaging, and electron microscopy. Sample...signal provided by smFPs can result in better resolution images. Conclusion By combining advantages ...

...cells (usually grown at 37 °C). Smart molecular evolution led to the identification of FLPe which has a ...sites (Schnütgen et al. 2003). Flex vectors revolutionize neuroscience Manipulation of specific cell types...channels (plasmid # 20298 for example) have revolutionized Neuroscience. They are the only means to target...transfected with both vectors. Flex vectors are the solution. Flex vectors expressing fluorescent cytoplasmic...

...differently from you and you will arrive at unique solutions to the same problem. If your lab/company/team ...because they will fight more adamantly for their solution), selecting a devil’s advocate is a good option...timer for five minutes and come up with as many solutions as you can in that timeframe. Tell yourself they...slowly turn your unworkable idea into a viable solution.  Anticipate Change Just because a process is ...

... yourself for potential gRNA sites, or you can utilise several online tools that do it for you. One such...by enhanced green fluorescent protein (EGFP) reconstitution. (a) Scheme of validation for DSB mediated ...mediated EGFP expression cassette reconstitution. When the target sequence was digested by sgRNA guided Cas9 ...is much easier and doesn’t require the extra precautions needed when dealing with RNA (e.g. face mask,...

...the fluorescence pattern is equivalent to the distribution of FP-fusion proteins. Our studies and those... increase in fluorescent signal achieved when utilizing an optimized moxFP as opposed to standard FPs....moxFPs currently represent one of the most inert solutions for imaging in a variety of cellular compartments...cellular organelles: serious pitfalls and some solutions." DNA and cell biology 32.11 (2013): 622-627. ...

...it. Several of these limitations and possible solutions are discussed below. There are numerous GFP variants...fluorescent analogues are generally added to the bathing solution and require uptake by the cells. Which of these...also a better choice for thicker samples. Another solution to avoid autofluorescence is offered by bioluminescent...fluorescent proteins. Luckily there are often solutions for many of the limitations, and the number of...

...you’re an academic researcher at a nonprofit institution, you can order plasmids covering a wide range...Institute for Molecular Bioscience has provided a solution for this problem by developing a general translation...next generation sequencing in order to study evolutionary dynamics. By counting DNA sequence reads, FREQ-Seq...surprisingly large fitness effects during adaptive evolution of antibiotic resistance." Proceedings of the ...

...anticipated nearly every webcam recording only 1280x720 resolution at best. Some came in even lower.  Learn: While...but due to costs and availability, a traditional solution was completely out of reach for Addgene.  So, ...Addgene. It’s why we exist, storing plasmids, and distributing them to labs that need them. The lab re-opened...Space was at a premium, so I had to figure out a solution for that. Normally, shots require me to get in...

...purpose: fast growth, high-throughput cloning, routine cloning, cloning unstable DNA, preparing unmethylated... Hte   High transformation efficiency deoR constitutive expression of genes for deoxyribose synthesis...disruption of arabinose metabolism pathway Inability to utilize arabinose as a carbon source leuB β-isopropyl...XL1 Blue Tetracycline Blue/white screening and routine cloning, nalidixic acid resistant. endA1 gyrA96...

...-localized red GECIs, enabling better spatial resolution for neuronal populations in vivo in both mouse...imaging of neuronal populations at single-cell resolution. PLoS Biol. 23(4): e3003048. https://doi.org/...reduced. This seemed to be due to the adipose cells utilizing glucose to the exclusion and detriment of the ...experiments By Ashley Waldron CRISPR/Cas9 has revolutionized biotechnology — partly because of its relative...

... the University of California, San Diego. The revolution in genetic engineering techniques is a speculation...the noteworthy work of fiction that it inspired. CAUTION: Spoilers alert! Pre-DNA Era  H.G. Wells, the...negative. But these negative depictions serve as cautionary tales for the entire scientific community and...Trading Card seen in this blog post?Download high resolution versions here.  Many thanks to our guest blogger...

...generation is safer than the first one and can be used routinely in a research laboratory. The risk of generating...used to express your gene of interest either constitutively or inducibly. If your gene of interest is toxic...cells - even your own! You should therefore be cautious about inserting potentially harmful genes into...information and always be sure to consult your institution’s Biosafety Committee before beginning any work...

...perspectives, the optimism they encapsulate, and the solutions they promote help motivate you to spend a little...from celebrities falsely claiming vaccines cause autism to politicians saying that climate change is a ...skepticism to promote harmful policies that benefit polluting industries.In March, three climate scientists ...citizens first and scientists second and that it is our duty to tell our science stories in a manner that encourages...communities at Harvard University to studying an evolutionary arms race at University of California, Berkeley...

...preparation for our upcoming affinity reagent distribution launch - and we're excited to say our long naming...to help us with our upcoming affinity reagent distribution launch! There was only one problem - we didn't... biggest discovery yet: Addgene will soon be distributing affinity reagents. Name Our New Antibody Friend...

... room above), but as Joanne Kamens, Addgene’s executive director, said during our celebratory lunch, it...’ve enjoyed seeing scientists sharing their contributions on social media as well the excitement amongst...community. For example, we started a new service distributing ready-to-use viral preps in 2016 and we just...