We narrowed to 65 results for: Uty

... Distribution to Industry Featured Collections Search Plasmids...Purification Optimization Kit Using SUMO Protease Elution Protein Characterization Christopher Bahl A validation...agreement between the recipient and the depositing institution(s). It governs the transfer of biological materials...distribute plasmids on behalf of the depositing institutions. These agreements indicate the language which...which we are allowed to use for distribution. Please see the Sample Agreements on our Technology Transfer... by an authorized signatory at the recipient institution. An authorized signatory is someone who can execute...execute legal documents on behalf of the recipient institution. Once the MTA has been signed, it can be submitted...

...iAChSnFR none Constitutive 1 Loren Looger 137955 pAAV.CAG.iAChSnFR CAG iAChSnFR none Constitutive 1, 9 Loren...none Constitutive 1, 9 Loren Looger 179254 AAV-CAG-jGCaMP8f-WPRE CAG jGCaMP8f none Constitutive 1, 9, ...none Constitutive 1, 9 Loren Looger 179256 AAV-CAG-jGCaMP8s-WPRE CAG jGCaMP8s none Constitutive 1, 9, rg...none Constitutive 1, 5, 9 Loren Looger 176757 AAV-EF1a-jGCaMP8m-WPRE EF1a jGCaMP8m none Constitutive 1, 9...none Constitutive 1 Lin Tian 112000 pAAV-hSynapsin1-axon-GCaMP6f Syn axon-GCaMP6f none Constitutive 1 Lin...none Constitutive 1 Oliver Griesbeck 173044 AAV-Camk2-NES-Twitch-GR CamKII Twitch none Constitutive 2 Yiyang...dLight1.1 none Constitutive 1 Lin Tian 111067 pAAV-CAG-dLight1.1 CAG dLight1.1 none Constitutive 1, 5 Lin Tian...

...gfaABC1D tdTomato Constitutive 5 Baljit Khakh 50465 pAAV-hSyn-EGFP hSyn EGFP Constitutive 1, 2, 5, 8, 9, ...tdTomato Constitutive 5 Hongkui Zeng 58909 pAAV-GFAP104-mCherry GFAP104 mCherry Constitutive 5 Edward ...PI.eGFP.WPRE.bGH GFA104 EGFP Constitutive 5 Philip Haydon 104055 pAAV-CAG-eYFP CAG EYFP Constitutive 2, 5, rg*, PHP....NLS-GFP Constitutive PHPeB Viviana Gradinaru 116869 pAAV-CAG-H2B-GFP CAG H2B-GFP Constitutive rg* Loren...H2B-tdTomato Constitutive rg* Loren Looger 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Constitutive 1, 2, 5,...ffLuciferase Constitutive 8 James M. Wilson 105534 pAAV.TBG.PI.LacZ.bGH TBG LacZ Constitutive 8 James M....TBG None Constitutive 8 James M. Wilson 105539 pAAV.hSyn.eGFP.WPRE.bGH Syn EGFP Constitutive 1 James M...

...mScarlet Constitutive 9 Christopher Harvey 59170 pAAV-Syn-Chronos-GFP Syn Chronos GFP Constitutive 1, 5, ...ChETA EYFP Constitutive 9 Karl Deisseroth 135633 pAAV-S5E2-C1V1-eYFP E2 C1V1 EYFP Constitutive 1, 5, 9, ...mCherry Constitutive 8 Roger Tsien 99039 pAAV-CamKII-ArchT-GFP (PV2527) CaMKII ArchT GFP Constitutive 1, 5...EYFP Constitutive 2, 5 Karl Deisseroth 137151 pAAV-nEF-NpHR3.3-EYFP nEF NpHR 3.3 EYFP Constitutive 8 Karl...dependent Flp dependent Cre and Flp dependent Constitutive Serotype AAV1 AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB...PI 20071 pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9 Karel Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell...mCherry-Fishell-3 Dlx ChR2 mCherry Constitutive 1, 9, rg* Gordon Fishell 135634 pAAV-S5E2-ChR2-mCherry nEF...

... or mammalian expression (Constitutive or Cre-dependent) Directed Evolution of a Selective and Sensitive...and Archon 2 (Constitutive or Cre-dependent) A robotic multidimensional directed evolution approach applied...Fast Genetically Encoded Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded...High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance calcium sensors...calcium sensors for imaging neural activity (Constitutive or Cre-dependent) Ultrasensitive fluorescent...Yellow protein calcium sensor jYCaMP1 variants (Constitutive or Cre-dependent) jYCaMP: an optimized calcium... Imaging intraorganellar Ca 2+ at subcellular resolution using CEPIA. Nat Commun. 2014 Jun 13;5:4153. ...

...Activity Serotype PI 99118 pAAV-CAG-tTA CAG-driven, constitutive Expression of the tet-off transactivator (tTA...34910 paavCAG-pre-mGRASP-mCerulean CAG-driven, constitutive Expression of pre-synaptic mGRASP-mCerulean ...34912 paavCAG-post-mGRASP-2A-dTomato CAG-driven, constitutive Expression of post-synaptic mGRASP-dTomato for...AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA CMV-driven, constitutive Expression of human codon-optimized Cas9 from...Serotype PI 149415 pAAV-TBG-ER-TurboID TBG-driven, constitutive Expression of ER-localized TurboID (with HA ...Serotype PI 58376 pAAV/D377Y-mPCSK9 hAAT-driven, constitutive Expression of mutant (D377Y) murine PCSK9 for...-hSyn-Kir2.1WT-2A-mScarlet-KASH hSyn-driven, constitutive hSyn-driven expression of Kir2.1 and mScarlet-KASH...

...have been mutated and screened through directed evolution to charge the tRNA with a different amino acid...and evolve proteins for various purposes from therapeutics to biopolymers. Tips for Success Before beginning... IBBN (G2) synthetase M. jannaschii 4-(2′-bromoisobutyramido)-phenylalanine (IBBN) and structurally analogous...Howard Hang 126035 pEVOL-ABK PylRS M. barkeri 3’-azibutyl-N-carbamoyl-lysine (AbK) Bacterial TAG Andrea ...Expresses yeast TrpRS/tRNA pair instead. For directed evolution of the liberated EcTrpRS/tRNA pair to incorporate...

...gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no signs of slowing down. This prokaryotic...easy multiplexing/scaling. Prokaryotes have long utilized CRISPR as a powerful defensive strategy against...CRISPR as part of an adaptive immune system - utilizing various CRISPR-associated (Cas) genes to not only...subtypes based on shared characteristics and evolutionary similarity. These are grouped into two large...technologies. Biotechnology companies are exploring therapeutic applications of CRISPR to treat genetic disease...ethics associated with CRISPR in agricultural and therapeutic applications. Despite the ethical controversies...truly disruptive technology for basic research. The beauty of CRISPR is that it’s adaptable to almost any ...

...close enough to cause a DSB. The nickase system is utilized in many CRISPR systems to achieve specific genetic... in nature, but have been created by directed evolution of the Escherichia coli TadA, a tRNA adenosine...window. These systems are well-suited to directed evolution applications. Examples of these base editing systems...activity. Knockout libraries often target 5′ constitutively expressed exons, while activation and repression...repression, many CRISPR epigenetic tools require constitutive expression of the dCas9 fusion and/or expression...CAPTURE system, developed by the Jian Xu lab , utilizes biotin tagging of dCas9 by fusing a biotin acceptor... Kim, D., Kim, S., & Kim, J. (2018). Directed evolution of CRISPR-Cas9 to increase its specificity. Nature...

...They are also an important tool for developing therapeutic strategies to treat Rett syndrome and restore...disease and for the development of successful therapeutics. ATCC - (Link opens in a new window) LUHMES ...CRISPR Tools CRISPR technology is a promising therapeutic approach for precise editing of mutations in ...Harvard Brain Bank (Link opens in a new window) Autism Brain Bank (Link opens in a new window) University... Hagberg et al. 1983. A progressive syndrome of autism, dementia, ataxia, and loss of purposeful hand ...Return to top Help us improve this resource by contributing your plasmids! To get started with your deposit...

...Neurotechnologies® (BRAIN) Initiative aims to revolutionize our knowledge of the human brain through the... behavior. Long desired by researchers, the revolutionary dynamic picture of the brain generated through...distributed as high-quality and high-titer 100 µL solutions of purified viral particles that are suitable ...which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter...which enables single cell, single millisecond resolution optogenetics. Cre-dependent virus Edward Boyden...distributed as 100 µL of purified viral particles in solution that are suitable for in vivo use....

...interference (RNAi) experiments at Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA... genes with potentially harmful mutations in therapeutic settings. As a tool in mammalian cell systems...retroviral and AAV backbone options. Plasmids for constitutive expression as well as those that allow for conditional... small interfering RNAs (siRNAs): mechanism, therapeutic targets, and delivery strategies for cancer therapy...

...Additional steps, including request approval, your institution’s approval of the MTA, and shipping, mean that... typically takes 1–4 days, depending on your institution. AAV Production 6–8 weeks Quality is our priority... and results through Addgene’s Data Hub . By contributing your AAV vector experience, from methods to ...lab, and even managing legal permissions and distribution. Troubleshooting If your answer isn’t in the...

...your specific application. Knockout : Target constitutively expressed regions, 5’ exons, or an exon known...cell type. Figure 2: Different delivery systems utilized for CRISPR/Cas gene editing. This image summarizes... frequently use AAVs to develop CRISPR-based therapeutics. One limitation of AAV vectors is that the packaging...will ultimately depend on which CRISPR method you utilized. If your edit affects genome sequence, your edited...Chen, P. J., & Liu, D. R. (2022). Designing and executing prime editing experiments in mammalian cells. ...

...Briefly, 293T cells are transduced with serial dilutions of a lentiviral vector, incubated for 72 h, and...293T cell line was obtained from Takara, and is routinely tested for mycoplasma contamination using mycoplasma... assay involves transducing cells with serial dilutions of the lentiviral vector, harvesting cells several...lentiviral insert. During this cultivation, cells are routinely checked for signs of microbial contamination. ...

...Garry Nolan 12257 pWPXL 2nd EF-1alpha-driven constitutive transgene expression. Contains intron that gives...Didier Trono 12254 pWPI 2nd EF-1alpha driven constitutive transgene expression and EGFP coexpression. ...Puro DEST 3rd Gateway destination plasmid with constitutive or inducible expression of cDNAs, shRNAs or ...shRNA or cDNA expression, gateway plasmid, and constitutive hygromycin selection. See Shin et al. (2006)...

...like rTetR, rtTA, and tTA. Dox has good tissue distribution, low toxicity, a known half-life (24 hours),...rtTA-Advanced TRE3G Johannes Zuber 60495 pSBtet-GP Sleeping Beauty transposon system with luciferase in cloning site... vector expressing Tet-On 3G transactivator constitutively expressed by EF1a promoter Tet-On 3G rtTA David...population. Proliferation history is monitored by the dilution of a dox-inducible H2B-mCherry marker. Aviv Regev...system for regulated gene expression through viral evolution . Gene Ther, 13 (19), 1382–1390. https://doi.org...

...storage. Biosafety Guide Documentation for your institutional biosafety office and your lab, information on...Vector Core have been transferred to Addgene for distribution. Look up the IDs of particular Penn Vector Core... have the right to use the original plasmid. Distribution of the original plasmid is important because...

...networks involved in retinal development the lab utilizes a CRISPR/Cas9 based approach to knockout, repress...listed here are provided as empty backbones that utilize Golden Gate cloning to insert the desired guide...guides can be expressed from the same plasmid by utilizing one of the dual guide shuttle plasmids to insert...

...some options for moving forward with a helpful solution. You must reply to that email within 10 business... weeks after the approval of the MTA by your institution. Our final step of the QC process, viral genome...some options for moving forward with a helpful solution. You must reply to this email within 10 business...