We narrowed to 766 results for: Vars

...fused a different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions...them. Thus, it has been widely adapted for a wide variety of applications: base editing, CRISPR activation... and results were consistent with copy number variation (CNV) analysis of the U2OS cells.   Figure...

... are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent...In mammalian cells, one of the improved folding variants of EGFP like mEmerald or Clover is probably best...measurements. Here, EGFP outperforms the improved folding variants, presumably due to the lower growth temperature...fellow at the Bauer Center for Genomics Research at Harvard University.       ...

...expression (Zhou et al., 2017). The most common variant is an 11 bp deletion in the B or C domain of one... ITR sequences from different AAV serotypes that vary in their RBE and T-shaped hairpin loop sequences...non-AAV2 ITRs can be used to drive gene expression at varying capacities. AAV vectors containing the AAV2 ITRs...different combinations of ITR and capsid serotypes vary in their gene transfer efficiency. Our practical...

...the best advice is to try a couple of promising variants to check how they perform under your experimental... a fluorescent protein (fusion) there is huge variation in the fluorescence intensities of individual ... plasmid uptake by cells. To correct for this variation, a reference fluorescent protein that is expressed... from Addgene. Another way to dodge cellular variation observed in mammalian cells is to tag an endogenous...

...well-characterized. During this time, we’ve also created a variety of educational resources in an effort to make scientific...created a normalization mechanism to “collapse” variations and synonyms of the same term that appear in ...promoter “Syn”, that, when selected, will match variations present in the data such as synapsin, hsyn synapsin...synapsin, hSynap. Matching We capture multiple variations of the same information in our search indices...

...gap in the field. Arlene Sharpe’s group at the Harvard Medical School developed a solution: CHIME, a CRISPR-Cas9...in vivo, the non-immunogenic violet-excited GFP variant Vex. To understand the impact of this gene editing...immune system, the group inserted sgRNAs targeting various innate and adaptive immune genes into pXPR_053.... Various Addgenies...

...and industry vary drastically, he reassures that one can learn it by reading a broad variety of materials...entrepreneurs must clearly communicate their idea at various levels of complexity and abstraction and convince...funding, foundations, and investors are examples of various sources of funding. Always ask for money. Moreover...

...see. To solve this issue, researchers conjugate various labels to antibodies that produce detectable signals...are great for multiplexing and can be used in a variety of assay types. Be sure to check the spectral properties...Peroxidase (HRP) and Alkaline Phosphatase (AP). Various substrates for each of these enzymes allow you ... methods for antibody conjugation can be quite variable from antibody to antibody or batch to batch. For...

...CRISPR-Cas9 activity switching via a novel optogenetic variant of AcrIIA4.” CASANOVA responds to blue light quickly... robotic picking approach to select cells with variant characteristics they wanted, the team identified...which may lead to unequal gene dosage and high variability of protein coexpression. By introducing a single... Various Addgenies...

...address these issues independently; for example, Harvard’s Therapeutics Graduate Program has a compulsory...Associate at the Labor and Worklife Program at Harvard Law School. She recently launched a survey “Identifying...Associate at the Labor and Worklife Program at Harvard Law School. He has continually promoted the cause...David T. Riglar! David T Riglar is a postdoc at Harvard Medical School. In the lab, he uses synthetic biology...

...universities typically requires funding from a variety of government and academic institutions. New graduate...grant writing, volunteered to take the lead on various applications throughout my graduate career, and... towards my PhD project. Grant writing offers various skill building opportunities that graduate students...readers why and how your innovation will address a variety of challenges and impact the field - its encouraged...

... using a dCas9 variant as well. Furthermore, they provide a high-fidelity (HF) variant of the BE3 that...Streptomyces  Streptomyces bacteria produce a wide variety of bioactive natural products. To easily explore...of essential and non essential genes in a wide variety of bacteria including in pooled, genome-wide, formats...bacteria. The authors were able to engineer a dCas9 variant fused to bacterial enhancer binding protein required...

...of neuronal networks at the single-cell level. Various soCoChR plasmids described in this work including...this strain in minimal media supplemented with various caffeinated beverages, but not with caffeine-free... stability allowing for minimized basal FRET variability, and is a good choice for FRET assays where a...  Various Addgenies...

...concentrations in different subcellular compartments can vary greatly. Additionally, the calcium changes you will...between experiments can be more difficult due to variance in sensor expression if you aren’t working with...through FRET sensors, can reduce these unwanted variables. Twitch is a FRET-based calcium reporter derived...advantage: tissue-specific targeting. By employing various AAV serotypes, researchers can precisely direct...

...Cas protein variants may also be useful for targeting ncRNAs. For example, the Cas12a variant can cleave...genomic elements (Hazan and Bester 2021). Other Cas variants Another option is CRISPR/Cas13, which targets ...specifically edit or degrade targeted lncRNAs. The Cas14 variant is a smaller and more versatile option. It can ...

...of yeast terminators of varying strength. A discussion of the effects of various terminators in the context...important role in RNA processing and contributes to variability in RNA half-life, and ultimately gene-expression...transcript. The purpose and mechanism of polyadenylation vary across cell types, but polyadenylation generally...

...or RNA) to target cells has been challenging. A variety of vector delivery technologies have been investigated...vectors great gene delivery tools? There are a variety of key properties that distinguish AAVs as gene...multitude of known AAV serotypes that have slight variations in their viral capsids. Novel forms of AAV capsids...are under consideration, recent advancements in various AAV technologies have vastly improved AAVs’ potential...

...results. Since polyclonal antibodies contain a variety of clones and thus the potential to bind many different... tend to have a strong signal and to work in a variety of applications. The desired specificity of your...your primary antibody. Manning et al. tested a variety of commercially available secondary antibodies ...factor Biological systems tend to be complex with a variety of proteins interacting. Experiments tend to be...

...proteins (FPs) came into wide use, there were a variety of ways to monitor cell, organelle, and protein...progressively longer blue fluorescence maturation times and varied blue-red maturation times (Table 1). These separate...useful for monitoring cellular events occurring at varied time scales.  Table 1: Monomeric FP Timers and ...and release from the plasma membrane by fusing various vesicle cargoes to FP timer mk-GO (Tsuboi et al...

... and this nuclease-null or “dead” Cas9 (dCas9) variant can then be fused to effector domains such as transcriptional...limitations to a system that relies on two distinct Cas9 variants—nuclease-competent or nuclease-null—in order to...with both the nuclease-positive and nuclease-null variants of Cas9 along with the necessary gRNAs, but it...control which gRNA becomes complexed with which variant of Cas9. To circumvent this problem, previous studies...