We narrowed to 268 results for: aav
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TypeCollection...Localization Optogenetics Plasmids Viral Service: AAV Biosensors Fluorescent biosensors are proteins designed...associated with the article. We also offer ready-to-use AAV preparations of select plasmids; these are noted ...Commun. 2018 Oct 25;9(1):4440. Eric Schreiter Calcium AAV expression of photoconvertible fluorescent protein-based...2024.12.16.628673. Olivia Masseck Calcium Astrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent...voltage imaging. Science. 2019 Aug 1. pii: science.aav6416. Eric Schreiter Voltage Green fluorescent voltage...
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CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...them advantageous for small capacity vectors like AAV. In addition to the mammalian option described below... -
Tetracycline Inducible Expression
TypeCollection...pTREtight promoter None Either Elledge 35625 pAAV-Ptet-RFP-shR-rtTA AAV; shRNA cloning vector; for evaluation... interest. tTA Off Sabatini 92099 AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep Bidirectional promoter controls expression...System. Contains homology arms for integration into AAVS1 Genomic Safe Harbor Locus. Tet-On 3G On Doyon 58245... -
Molecular Biology Reference
TypeGuide...viral particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that can infect your target...expanding viral service offers select ready-made AAV and lentiviral particles. Visit our viral service... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post...respectively, to obtain PEs small enough to deliver with AAVs but still efficient at long and complex edits. The... -
Deep Dive: qPCR
TypeBlog Post...resources on the Addgene blog Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR... -
Deisseroth INTRSECT Collection
TypeCollection...targeting strategy that allows adeno-associated virus (AAV)-borne payloads to be expressed in cells based on... Items 55636 pAAV-EF1a-Cre None Yes 55632 pAAV-Ef1a-mCherry-IRES-Cre None Yes 55637 pAAV-EF1a-Flpo None...None Yes 55634 pAAV-EF1a-mCherry-IRES-Flpo None Yes 55638 pAAV-EF1a-vCre None Yes 55635 pAAV-EF1a-sCre None...Items 55641 pAAV-Ef1a-fDIO EYFP Flp Yes 55640 pAAV-Ef1a-dDIO hChR2(H134R)-EYFP Dre No 55639 pAAV-Ef1a-fDIO...55650 pAAV-hSyn Con/Fon EYFP Cre AND Flp Yes 231926 pAAV-Ef1a-Con/Fon-EYFP Cre AND Flp No 55651 pAAV-hSyn...137134 pAAV-Ef1a-Coff/Fon-mCherry Flp AND NOT Cre Yes 137135 pAAV-Ef1a-oScarlet None Yes 137136 pAAV-Ef1a-Con... 137125 pAAV-Ef1a-sRGECO None Yes 137126 pAAV-Ef1a-Con/Fon-sRGECO Cre AND Flp Yes 137127 pAAV-Ef1a-Con... -
CRISPR Guide
TypeGuide...efficiently packaged into adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9 ...Mammalian CRISPR libraries have also been created in AAV backbones for in vivo experiments and in a retroviral...capabilities but are small enough to be packaged in AAV particles. Cas13 fusions have also been used in in... -
CRISPR Pooled gRNA Libraries
TypeCollection...113584 (EFS) 113585 (TBG) Knockout Mouse Chen N/A (AAV) 4 286 Mouse Validation (mVAL) CRISPR Library 159391... -
Plasmids for Stem Cell Research
TypeCollection...Jun 6. Buganim Glial Cells Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-... -
CRISPR Guide
TypeCollection...efficiently packaged into adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9 ...Mammalian CRISPR libraries have also been created in AAV backbones for in vivo experiments and in a retroviral...capabilities but are small enough to be packaged in AAV particles. Cas13 fusions have also been used in in... -
Our New Antibody Mascot is....
TypeBlog Post... Last month, we introduced you to Blugene and Aavery's newest friend, our Addgene antibody mascot, here...on Feb. 24. Stay tuned! Original Blugene and Aavery would like your attention, please, as they introduce...AntibodyMakesThree on Twitter. As with Blugene and Aavery, we’re looking for a gender neutral name (and it... -
General Transfection
TypeProtocol...of HEK293T cells optimized for viral production (AAVpro or Lenti-X), but any HEK293T cell line should work... -
Typing CRISPR Systems
TypeBlog Post...362(6416), 839–842. https://doi.org/10.1126/science.aav4294 Hu, C., Myers, M. T., Zhou, X., Hou, Z., Lozen... 363(6422), 88–91. https://doi.org/10.1126/science.aav7271 Yoshimi, K., & Mashimo, T. (2022b). Genome ... -
Let There Be LITE Plasmids
TypeBlog Post...offers a tip: save yourself some time by making an AAV1 supernatant. (The details can be found here.) So... -
What's New in CRISPR - March 2020
TypeBlog Post...design and delivery. They generated hPSC lines with AAVS1-integrated, inducible, and fluorescent dCas9-KRAB... -
Hot Plasmids - October 2022
TypeBlog Post...available as Cre-dependent, EF1ɑ-driven viral vectors (AAV1) - and you can also find several other constructs... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog Post...situation thought to be suboptimal for HDR. At the AAVS1 locus, the two nearest gRNAs had cleavage sites ... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog Post...editing at three different genomic sites in the AAVS1 locus using GEmCherry2. In this experiment they ... -
Plasmids 101: The protein expression toolbox
TypeBlog Post...action Check out Addgene's Lentiviral Tet-on and AAVS1 targeted Tet-on transgene vector. Degron tags Tagging...