...mCherry expression using FACs sorting, confirming yet again that sgRNA structure and genomic recognition sites...

...editing speed dictates which phage variants can propagate faster than the rate of dilution (passage from...

...Additionally, align a custom nucleotide sequence against a given sequence using BLAST2. See this blog post...

...on our Viral Service (and then we crunched them again) to find the most requested lentivirus and AAV of...

...to methylation if the enzyme's recognition site (TCTAGA) is preceded by GA or followed by TC. As shown ...

... rules that I try to follow in my own research. Again, some of this may not apply or may not work for ...

...proteins inside a cell have a commercial antibody against them. There’s also a question about how specific...

...PTGs containing 8 gRNAs targeting 4 MAPK genes, again using rice protoplasts. Each pair of gRNAs targeted...

...like those pioneered by Ewen Mullins of Ireland’s Teagasc.  Plant biotechnology is full of potential, but...

...really helpful, perhaps you wouldn’t mind doing it again next month?” Some mentoring relationships grow naturally...

...systems that bacteria and archaea use to protect against these foreign nucleic acids. Acr family members...3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s..., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., ...: 27984730 Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013). Genome ...

...3-100 per cell, a stabilized promoter buffered against the effects of copy number on reporter gene expression...

...check the fragments size of your digestion on an agarose gel. If the digestion does not provide the fragments...

...constantly communicating with a tumor, proving once again that a tumor is always interacting with its surroundings...

...you!)  Amount of information and level of detail Again, this is an obvious one on the surface, but it’s...

...full-length protein that you want to generate antibodies against, or a unique peptide sequence derived from this...

...in r/molecularbiology.   Oh, to be young again! Addgenie Marcy (now our Sr. Director of Production...

...from a complex mix using affinity chromatography against an amino acid sequence, or tag, on the protein....

...found (typically RNA that the RNAi is designed against), RISC can repress translation and/or degrade target...

...localization of the hM4Di, we used primary antibodies against mCherry, and secondary antibodies coupled to nanogold...