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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 599 results for: arl

Showing: 241 - 260 of 599 results

  4. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...hairpin that can be designed to bind and detect nearly any RNA sequence. Downstream of the hairpin is ...26615910. PubMed Central PMCID: PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian...

  5. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...better serve the community. We are delighted at the early outcomes and the ecstatic reception by scientists...worry about getting support from depositors. We regularly hear how much they see the value in depositing...

  6. Addgene: The First Twenty Years

    Type
    Blog Post
    ...School. We want to give a special thanks to the early-adopter scientists, who trusted Addgene before we...— 10,000 plasmids deposited and available — and nearly seven before we had 1,000 depositing scientists...

  7. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...Cas12a cleavage results in 5 bp overhangs and is particularly convenient for targeting experiments requiring...it targets RNA instead of DNA. This makes it particularly useful in generating transient edits of signaling...

  9. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ... also be used to ensure specificity; this is particularly helpful when wanting to target specific neuron...Dystrophy. InTech. doi: 10.5772/53023 Veldwijk, Marlon R., et al. "Development and optimization of a real-time...

  10. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...Why study neural connectivity? One of the early lessons many of us learned in biology is that the body...Deshpande et al., 2013). The study found that at early time points, most of the projections to these new...

  13. A Look at Addgene's QC Process

    Type
    Blog Post
    ...differences are considered a QC issue is reviewed yearly at a best practices meeting, where the QC team ...pipeline. There are regions of plasmids that are particularly difficult to sequence and assemble using the...

  14. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...Zibitt et al. 2021). Mutating splice sites is particularly effective as it can result in the retention ...used to target lncRNAs with high specificity, particularly in applications requiring smaller delivery systems...

  15. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ... direct ELISA, aside from the detection step. Similarly, the protein antigen is coated onto the wells ...calculate the protein concentration. Image from Jagarlamudi et al., 2015.   The maximum OD that most ...

  17. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... close to the hood so you can view your cells regularly (both before entering the hood and when putting...Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology and Immunology. You...

  18. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7... and base editing, to more loci. Although these early data are exciting, there’s a lot we still don’t ...
Showing: 241 - 260 of 599 results