We narrowed to 600 results for: arl

...Therapies: CRISPR, Stem Cells, and Beyond meeting earlier this month. Another issue that arises for base...cannot be expressed until the base editor corrects early stop codons within T7 RNAP. A phage encoding the...

...best, wins. He explained that entrepreneurs must clearly communicate their idea at various levels of complexity...works. “We use functional spectrometers from the early 1990s that were free and free to repair.” If the...

...ends, some 50% of these groups continue to meet regularly, providing ongoing support, advice and accountability...most hits. Commit to a meeting schedule and meet regularly no matter what, even if one person can’t make ...

...School. We want to give a special thanks to the early-adopter scientists, who trusted Addgene before we...— 10,000 plasmids deposited and available — and nearly seven before we had 1,000 depositing scientists...

... also be used to ensure specificity; this is particularly helpful when wanting to target specific neuron...Dystrophy. InTech. doi: 10.5772/53023 Veldwijk, Marlon R., et al. "Development and optimization of a real-time...

...absorb the excitation light less efficiently. Similarly, decreasing the power of the excitation light ...experiments. As the photobleaching rate is not linearly related to power, the conclusions reached at high...

...unlike CRISPR. Early CRISPR labeling techniques allowed researchers to visualize nearly any single genomic...

...hairpin that can be designed to bind and detect nearly any RNA sequence. Downstream of the hairpin is ...26615910. PubMed Central PMCID: PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian...

...better serve the community. We are delighted at the early outcomes and the ecstatic reception by scientists...worry about getting support from depositors. We regularly hear how much they see the value in depositing...

...Cas12a cleavage results in 5 bp overhangs and is particularly convenient for targeting experiments requiring...it targets RNA instead of DNA. This makes it particularly useful in generating transient edits of signaling...

... to early career scientists using fluorescent proteins in their research. These awards help early career...

...differences are considered a QC issue is reviewed yearly at a best practices meeting, where the QC team ...pipeline. There are regions of plasmids that are particularly difficult to sequence and assemble using the...

...Zibitt et al. 2021). Mutating splice sites is particularly effective as it can result in the retention ...used to target lncRNAs with high specificity, particularly in applications requiring smaller delivery systems...

...Why study neural connectivity? One of the early lessons many of us learned in biology is that the body...Deshpande et al., 2013). The study found that at early time points, most of the projections to these new...

...your questions, data, and conclusions should be clearly identified and interpreted in less than a couple...these colors so that your text is easily read. Similarly, be cognizant of audience members that are color-blind...

... direct ELISA, aside from the detection step. Similarly, the protein antigen is coated onto the wells ...calculate the protein concentration. Image from Jagarlamudi et al., 2015.   The maximum OD that most ...

...could be repurposed for SARS-CoV-2 RNA detection. Early in the pandemic, labs around the world have been...  Expression of SARS-CoV-2 viral proteins In the early weeks of the COVID-19 pandemic, the Krogan lab at...

... to prevent dCas9 tethering to DNA. Since these early anti-CRISPR studies, scientists have discovered ...can increase the chance of off-target editing particularly after the majority of on-target editing has ...

...may be too constraining for some experiments, particularly in vivo ones. In those cases, AAV vector screens...your CRISPR screen to a high degree. This is particularly helpful when investigating tissue-specific diseases...

...cytometry (“spectral flow”) has emerged as a particularly useful tool for those wishing to run larger ...Mayo Clinic studying host-microbe interactions in early life.    Resources More resources on the Addgene...