AAV Production in HEK293 Cells
Type
Protocol
...pyruvate, Corning 10-014-CV (optional) 7.5% sodium bicarbonate, Corning 25-035-CI (optional) 1 M HEPES, HyClone... 7.5 mL 100X glutaGRO, 7.5 mL of 7.5% Sodium Bicarbonate, 7.5 mL 1 M HEPES to 750 mL DMEM + 1 g/L glucose...other components, such as sorbitol and sodium bicarbonate, sorbitol has been found to increase viral titers...