We narrowed to 376 results for: bre

1. Mentoring for Scientists: I Have a Mentor, What Now?

   Type

   Blog Post

   ...Figure out what I want to do after I get my PhD) and break them down into smaller, actionable steps (e.g. Meet...

2. The Power Behind NGS Plasmid Validation: seqWell

   Type

   Blog Post

   ... seqWell Inc. Plasmids and PCR products are the bread and butter of molecular biology labs the world over...

3. Reagent Repositories Are Speeding up Science During the Pandemic

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   Blog Post

   ...doi.org/10.1016/j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038...

4. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

   Type

   Blog Post

   ...presenter at their poster, they could be hosted in breakout rooms, or even on social media.Zhang was able ...

5. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

   Type

   Blog Post

   ...Addgene. The Podgorski Lab has provided a brief breakdown of the promoter systems you can use to express...

6. Networking Follow-up: I Met a Person. Now What?

   Type

   Blog Post

   ... multiple times if you don’t get a response. It breaks an important law of networking--don’t be creepy...

7. Four Factors that Differentiate the Stem Cell Field

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   Blog Post

   ...Parkinson's Disease; Hideyuki Okano presented ground-breaking work on spinal cord injuries; Jessica Young shared...

8. Choosing Your Fluorescent Proteins for Multi-Color Imaging

   Type

   Blog Post

   ...given application is a challenge. To help keep you abreast of the latest fluorescent proteins, I maintain ...

9. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   ...the histone methylation status at a double-strand break that needs repairing. They may be looking at chromatin...

10. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type

    Blog Post

    ...Comprehensive Set of MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell Reports, 2016. PubMed PMID: 27760319...

11. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    ... nuclease, which introduces double stranded DNA breaks, can be expressed from lentiCas9-Blast. Purpose...

12. Targeted Mutagenesis with EvolvR

    Type

    Blog Post

    ...tracing cells that do not tolerate double-strand DNA breaks by introducing a unique tag into a genomic site...

13. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type

    Blog Post

    ... it available to everyone, that creates more breakthrough and innovation. With any kind of open platform...

14. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    Blog Post

    ...These cells, presumably the cell you want, cannot break down X-gal and are white. Cells that don’t contain...

15. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type

    Blog Post

    ...be targeted by a sgRNA guided Cas9 causing a DSB break. This DSB is repaired by NHEJ which depending on...

16. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    Blog Post

    ...the DNA base change without a DNA double-strand break. But base editors aren’t perfect. They may be slow...

17. Building and Validating Recombinant Antibodies: A Case Study

    Type

    Blog Post

    ... Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. A toolbox of IgG subclass-switched...

18. Sweating the Small Stuff: Details in the Lab

    Type

    Blog Post

    ...intentionally slow down, you’re giving yourself a chance to break what can be a rather vicious cycle.  Everybody’s...

19. Viral Vectors 101: Viral Vector Elements

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    Blog Post

    ...components are called helper plasmids. Below, we’ll breakdown the vector components first by virus and then ...

20. Viral Vectors 101: Types of viruses

    Type

    Blog Post

    ...through nuclear pores and thus do not require breakdown of the nuclear envelope during cell division to...