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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 630 results for: emb

Showing: 241 - 260 of 630 results

  1. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...them. Collectively, the pool of CRISPR software embodies solutions that facilitate most experimental applications...in the table): Basic functions: These functions embody the main goals of the software and should be the...genomes are denoted by organism name and genome assembly version. The names in the table appear as they...organisms and will help you whether you have a full assembly or not. It is an offline tool, but comes with ...

  3. Lentivirus Plasmids

    Type
    Collection
    ...pSLCAR-CD19-28z 3rd Modular CD28-CD3z CAR backbone. See Bloemberg et al. (2020) for additional CAR backbones. Scott...

  4. CRISPR Guide

    Type
    Collection
    ...against these foreign nucleic acids. Acr family members inhibit CRISPR by a variety of mechanisms, including...Mediated Genetic Screening in Mice with Haploid Embryonic Stem Cells Carrying a Guide RNA Library. Cell ...

  5. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...This grows our relationship with our community members. We also find that, as a nonprofit, we attract ...is strength in diversity and in empowering team members to take initiative and give their best As we continue...Research Listen to Our Interview with Addgene Board Member, Connie Cepko Resources on Addgene.org Learn ...

  6. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...characteristics like pH, redox state, and voltage across membranes. These sensors generally follow the same pattern...an organelle-specific targeting signal or a transmembrane domain, biosensors can be targeted to a particular...endoplasmic reticulum (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted...

  7. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ... the pores being opened in the pathogen’s cell membrane (Dunkelberger & Song, 2010). As cells cannot survive... survive when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody...providing structure and isotype determination, remember that it can do so much more than that!    References...

  8. Fostering communication in remote collaborations

    Type
    Blog Post
    ...meetings include all collaborators, from the lab members who are conducting the experiments to the PIs. ...closely replicates how you’d interact with team members in person, and improves overall communication.  ... informal communication you’d likely have with members of your lab that you see in person, and helps improve...

  9. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]) of this membrane-embedding voltage detector. Find Archon1 and its cre-dependent...GABA sensor (pAAV.hSyn-FLEX.iGABASnFR) has good membrane localization and brightness. The F102G variant...

  11. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...10,000 users per month in November 2014 to ~30,000 users per month in November 2015. We will continue ...providing recorded interviews with Addgene community members. In addition we’ve experienced incredible growth...

  12. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...Tat. Although CPP fusions can translocate a cell membrane, the presence of the CPP may alter protein function...various loxP reporters. In both mouse and human embryonic stem cells, they found very high recombination...neuronal and gut stem cells, dendritic cells, embryonic fibroblasts, glia cells and neurons, and cell ...

  13. Lentivirus ddPCR Titration

    Type
    Protocol
    ...of DMEM complete instead of a viral dilution). Remember, this is an additional 10-fold dilution of the...

  14. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ... out these questions ahead of time so that lab members have time to prepare their thoughts. Then at the...Set up a brief bi-weekly meeting where all lab members talk no science. No data presentation will appear...remotely in science are endless. Let’s adapt and embrace new ways of working, reconnect as human beings ...

  15. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...differentiation, the Zhang lab transduced human embryonic stem cells with the viral TF library, with each...lentiviral particles and used to transduce human embryonic stem cells. Transduced stem cells were differentiated...this screen successfully differentiated cells resembling all three germ layers (ectoderm, endoderm, and...

  16. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...editing system to correct the cystic fibrosis transmembrane conductor receptor (CFTR) by homologous recombination...of CRISPR/Cas9." Cell Stem Cell. 13, 659-662 (5 December 2013). 8. Gerald Schwank et al. "Functional Repair...Fibrosis Patients." Cell Stem Cell. 13, 653-658 (5 December 2013).    ...

  17. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...Institute for Biologically Inspired Engineering and member of the Addgene Advisory board, on the difficulty...expressing Cas9. As John Doench (Broad Institute and member of the Addgene Advisory Board) says: "When you'... running. Both Alex Chavez and John Doench are members of the Addgene Advisory Board. Additional Resources...

  18. How to Lead a Great Meeting

    Type
    Blog Post
    ...everyone gets a chance to talk and less vocal team members still have their voices heard. Here's a template...of the agenda, and assign each one using group members’ initials. If appropriate, email this around after...to help keep you accountable for moving along. Remember, many of the more in-depth discussions can happen...

  19. CRISPR Plasmids - Tagging

    Type
    Collection
    ... use in SapTrap reactions for targeting vector assembly, and the remaining 5 plasmids include Cre and ...

  20. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...viral envelope, which fuses with the target cell’s membrane, allowing the viral particle access into the cell...inside the nucleus, without the need for envelope disassembly. Both lenti and retro viruses can accommodate...of murine leukemia virus DNA depends on mitosis.” EMBO J. 12 (1993) 2099-2108. PubMed PMID: 8491198. PubMed...
Showing: 241 - 260 of 630 results