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Showing: 241 - 260 of 631 results

  1. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...on the floor outside the lecture hall. It was wonderful to see the ad-hoc ‘sciencing’ when Dr. Prakash... By connecting art and science, a fascinating interface is created that produces loads of inspiration ...

  3. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...time needed to go from planning an experiment to performing one.  At Addgene, over 120 labs have deposited...we currently have data for are: cut, activate, interfere, visualize, nick, purify, tag, scaffold, CRISPR-display...

  4. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ... and serotype. Please note that Addgene cannot perform functional or in vivo testing. We recommend you...plasmid of choice for more information on its performance.   Titers We expect titers for plasmids packaged...

  6. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...tracrRNA sequences in a “single-guide RNA” that performs both functions. Cas9 cuts both the target and ...RNA guide (either crRNA or sgRNA). Cas9 requires perfect complementarity in this region between the guide...

  8. Hot Plasmids - October 2022

    Type
    Blog Post
    ...experiments with mouse cortical neurons, far out-performing previous attempts to engineer potassium-selective...engineering to shift the spectra and improve the performance of HcKCRs. Find HcKCR plasmids here!   Govorunova...

  11. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...You’ve designed the perfect experiment – controls, conditions, and everything in between – now all you...downstream applications. Tag cleavage must be performed after primary purification and requires a second...

  12. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...therapeutic antibody that binds Activin A and interferes with inappropriate activin-ACVR1 interactions...bring HOPE to AJ.   PS: The superhero race is the perfect event for supporting AJ, who is a big superhero...

  13. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...isolate single-cell colonies, grow them up, then perform individual genotyping (using Sanger sequencing,.... You could then confirm this purification by performing a genotyping assay (such as Sanger sequencing...

  14. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...prokaryotes originally utilized CRISPR/Cas as a powerful and adaptive defense strategy against life-threatening...The diversity of the CRISPR/Cas systems provides powerful lines of defense against invading phages. Rising...

  15. AAV Production in HEK293 Cells

    Type
    Protocol
    ...serotypes are harvested at 96–120 h. Optional: perform a media exchange at ~18 h post transfection. Changing...

  16. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...your choice. First, a viral vector is selected to perform the transduction with: either retrovirus, lentivirus...still express episomally long-term (AAV). If you perform a lipid-based or electrical-based transfection,...

  17. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...University. Stochastic multicolor labeling is a powerful solution for discriminating between neurons for... very stable in SeeDB2. However, SeeDB2 is not powerful enough for large brain samples. For large brain...

  18. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...development, and assay technologies makes her a perfect fit for Addgene. She was Head of Quality at Dimension...microRNA-based system for polymerase II-regulated RNA interference. In the first two months since publication, ...

  20. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...beta-galactosidase cassette allowing a researcher to perform blue-white screening after transformation - self...7798275. Additional Resources on the Addgene Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis...
Showing: 241 - 260 of 631 results