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We narrowed to 636 results for: erf

Showing: 241 - 260 of 636 results

  2. Hot Plasmids - October 2022

    Type
    Blog Post
    ...experiments with mouse cortical neurons, far out-performing previous attempts to engineer potassium-selective...engineering to shift the spectra and improve the performance of HcKCRs. Find HcKCR plasmids here!   Govorunova...

  3. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...You’ve designed the perfect experiment – controls, conditions, and everything in between – now all you...downstream applications. Tag cleavage must be performed after primary purification and requires a second...

  4. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...tracrRNA sequences in a “single-guide RNA” that performs both functions. Cas9 cuts both the target and ...RNA guide (either crRNA or sgRNA). Cas9 requires perfect complementarity in this region between the guide...

  6. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...therapeutic antibody that binds Activin A and interferes with inappropriate activin-ACVR1 interactions...bring HOPE to AJ.   PS: The superhero race is the perfect event for supporting AJ, who is a big superhero...

  7. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...prokaryotes originally utilized CRISPR/Cas as a powerful and adaptive defense strategy against life-threatening...The diversity of the CRISPR/Cas systems provides powerful lines of defense against invading phages. Rising...

  8. AAV Production in HEK293 Cells

    Type
    Protocol
    ...serotypes are harvested at 96–120 h. Optional: perform a media exchange at ~18 h post transfection. Changing...

  9. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...development, and assay technologies makes her a perfect fit for Addgene. She was Head of Quality at Dimension...microRNA-based system for polymerase II-regulated RNA interference. In the first two months since publication, ...

  10. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...University. Stochastic multicolor labeling is a powerful solution for discriminating between neurons for... very stable in SeeDB2. However, SeeDB2 is not powerful enough for large brain samples. For large brain...

  12. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...your choice. First, a viral vector is selected to perform the transduction with: either retrovirus, lentivirus...still express episomally long-term (AAV). If you perform a lipid-based or electrical-based transfection,...

  13. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...beta-galactosidase cassette allowing a researcher to perform blue-white screening after transformation - self...7798275. Additional Resources on the Addgene Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis...

  17. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...term for a range of neat little tricks our minds perform to avoid cognitive dissonance (Kunda, 1990). More...scientific research is funded by the public and performed by researchers who received their educations from...

  18. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...the gradual change in pH visible at the sample interface upon thaw (Fig. 2). Before thawing, all the samples...study found that dry ice storage affected the performance of antibodies, because of effects of pH on protein...

  19. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...evaluation of ‌therapeutic immunogenicity is typically performed using traditional 2D assays with immune cell lines...of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 130, 128–142. https://doi.org/10.1016...

  20. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...academia, is someone who visits an institution to perform research there temporarily. The length of stay ...relevant literature for the techniques you will be performing to familiarize yourself. Identify reagents you...
Showing: 241 - 260 of 636 results