We narrowed to 326 results for: iNOS

1. Fluorescent Proteins 101: Introduction to FRET

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   Blog Post

   ...end positions without changing the order of the amino acids in the protein) may be able to boost FRET ...

2. Plasmids for Endogenous Gene Tagging in Human Cells

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   Blog Post

   ... which terminus, N or C, to tag and which short amino acid linker to use. These decisions were made based...

3. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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   Blog Post

   ...Thomas, D., & Surdin-Kerjan, Y. Metabolism of sulfur amino acids in Saccharomyces cerevisiae. Microbiol Mol...

4. Hot Plasmids and Viral Preps - March 2021

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   Blog Post

   ...fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible degron sequence) tagged proteins...

5. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   ... editing efficiencies of the chimeric and fully albino mice ranging from 11% to 100%, a level similar ...

6. Mouse Modeling, Part 1: Genetically Engineered Mice

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   ...first blog post in this series, I’ll highlight terminology you should be familiar with before working with...

7. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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   ...Szikra, T., Esposti, F., Cowan, C.S., Bharioke, A., Patino-Alvarez, C.P., Keleş, Ö., Kusnyerik, A., Azoulay...

8. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   ...doi.org/10.1038/nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

9. 6 Tips for Grant Writing

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   Blog Post

   ...other scientists that we forget that some of the terminology we use routinely isn’t common to non-scientists...

10. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post

    ...was not possible, but one of our members took an Arduino microcontroller and a motor controller and made...

11. Plasmids for Stem Cell Research

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    ... reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells. Nat Biotechnol...

12. Tetracycline Inducible Expression

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    ... et al. used random mutagenesis to identify the amino acid residues of TetR that were important for tetracycline-dependent...

13. Hot Plasmids: Summer 2025

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    ...small enough to be carried by viral vectors (~400 amino acids, less than a third of the size of Cas9). ...

14. Viral Vectors 101: Producing Your rAAV

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    ... https://doi.org/10.1089/hum.2010.107 Zen, Z., Espinoza, Y., Bleu, T., Sommer, J. M., & Wright, J. F. ...

15. AAV Vector Quality Control: Going the Extra Mile with NGS

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    Blog Post

    ...viral production. This recombination produces a minor pre-recombined population that does not require ...

16. Truncated gRNAs for Regulating Gene Expression

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    ...additional effector domains (4). Taken together, these minor gRNA alterations can be exploited to quickly and...

17. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post

    ...bacteria where cleavage leads to the loss of spectinomycin resistance. xCas9 3.7 showed the broadest PAM...

18. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ...different trinucleotides (corresponding to different amino acids) in the regions that bind to the antigen. ...

19. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ...blastx results start the alignment at a more distal amino acid. We consistently run our blastx searches with...

20. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    Blog Post

    ...secondary peak around 280 nm thanks to aromatic amino acids like tryptophan and tyrosine (Figure 1B). ...