Skip to main content
Addgene
Showing: 241 - 260 of 532 results

  1. All in a Twist: dsRNA

    Type
    Blog Post
    ...generate dsRNA by bidirectional transcription or as inverted repeats. These elements are almost always epigenetically...dsRNAs is. A major source of dsRNA (that doesn’t involve cellular dysregulation) is the mitochondria. These...

  2. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...and postdocs may feel they have limited funds to invest in this. However most coaches who focus on scientists...to help. Don’t be afraid to ask and to make an investment in your future. Additional Resources on the ...

  3. Plasmids 101: Cre-lox

    Type
    Blog Post
    ... location and orientation of the loxP sites: Inversion: If the loxP sites are on the same DNA strand ...opposite orientations, recombination results in an inversion and the region of DNA between the loxP sites is...

  4. Hot Plasmids February 2024

    Type
    Blog Post
    ...adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery in non-human primate (NHP...This new capsid will be a valuable tool for investigating NHP brains. Figure 3: Overview of library...

  6. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...flexibility of the single-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying... viral preps available!  We are expanding our inventory of ready to use viral preps from the Deisseroth...

  7. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ... of outcomes including excision or insertion, inversion, translocation or cassette exchange. By utilizing... clade of huge phages. In this article, they investigated three Casɸ orthologs, Casɸ-1, Casɸ-2, and Casɸ...

  10. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...you want combined in your offspring. Genotyping involves getting a small piece of tissue from each mouse...effectively breeding your mice. Because their generation involves the integration of a novel gene randomly into ...

  11. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...depositing scientist, you will have the most involvement with Step 1, and, while data entry can be a chore...Distribute pending QC. The name of the Principal Investigator and Organization where your plasmids were first...

  12. 6 Tips for Grant Writing

    Type
    Blog Post
    ...intend to be productive with the granting agency’s investment. 6. Failure is part of the process, keep your...applications there’s going to be an element of luck involved so it’s important not to throw in the towel if...

  14. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ... factor What is fun for the audience? Getting involved and feeling "closer" to the panel. Your audience...incorporate them into the program. I have to admit to inviting interesting friends and colleagues to events for...

  16. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...that change single base pairs, to chromosomal inversions and large deletions. Many of these methods rely...However, there is the chance of a chromosomal inversion, or just random mutations if the chromosome is...

  17. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...recommend using primers targeting the viral genome or inverted terminal repeats (ITR). You should always have...quantification of adeno-associated virus serotype 2-derived inverted terminal repeat sequences. Human Gene Therapy ...

  18. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...autoclaving, which also takes time. Rather than investing all of this effort in preparing reagents, I choose...quantification of adeno-associated virus serotype 2-derived inverted terminal repeat sequences. Human gene therapy ...
Showing: 241 - 260 of 532 results