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We narrowed to 809 results for: mal

Showing: 241 - 260 of 809 results

  1. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ... the economic and cultural importance of these animals to human populations around the world. Here at ...take a moment to revisit some of the ways these animals have impacted humans through biomedical research... time, researchers were already on a quest for smaller forms of antibodies and had begun developing Fab...fragments and scFvs. But the potential for an even smaller, single-domain antibody fragment was exciting, ...  Excellent tissue penetration, thanks to their small size of 12–15 kDa Ease of cloning and expression...

  2. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ... new page shows you base editing plasmids for mammalian systems, bacteria, plants, and yeast. Popular ...Targeting: This page contains Cas13 constructs for mammalian and bacterial systems. Plasmids with wild-type...unique non-specific RNA cleavage in bacteria. In mammalian systems, Cas13a degrades only the specified RNA...anyone predict that we’d be able to edit RNA in mammalian cells with CRISPR? I certainly didn’t, but now...Genotyping Day 5: Delivery systems: CRISPR 101: Mammalian Expression Systems and Delivery Methods and A ...

  3. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ...extreme physical and chemical conditions like YNP thermal sites - are also of practical interest since they...using a heat gun, and pH measurements. We collected small water and sediment samples, stabilizing them in ...hot spring sample. The sample was separated into smaller samples of 5-10 cells each for sequencing, allowing...IgnaviCas9 protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable... Cas9 from Parvibaculum lavamentivorans, whose optimal growth temperature is 30°C. Find IgnaviCas9 here...

  4. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...achieved by inserting BirA into a pMal plasmid (NEB) thus creating the pMal-T-Avi-His/BirA plasmid now available...use of the enzyme biotin ligase, which binds to a small (< 20 amino acid) AviTag sequence in a protein and...biotin ligase and a protein of interest fused to maltose binding protein (MBP, New England BioLabs). MBP...interferometry (Octet, Pall ForteBio). Tips for using pMal-T-Avi-His/BirA to add biotin to your protein of ...

  5. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...were unable to show TtAgo-mediated cleavage in mammalian cells, likely due to high temperature requirements...cleave plasmid and genomic DNA in a variety of mammalian cell lines, with an efficiency similar to that...sequence can greatly affect editing efficiency. Smaller size At only 887 amino acids in length, NgAgo is... feature is advantageous if you’re working with small capacity viral vectors such as AAV. Base removal...guides. While this fidelity would prevent other small nuclear DNAs from binding NgAgo, it might make it...

  6. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...of unhealthy, difficult-to-inject unc-119(ed3) animals, as well as a second injection step to remove the...select for candidate knock-ins. These candidate animals can then be further screened based on their Roller...the SEC and after 5-6 days of growth, wild-type animals that have undergone SEC excision are screened based...based on the loss of the Roller phenotype. These animals contain a fluorescent protein fusion for the targeted... 7 genes in parallel; when they injected 60-80 animals per gene, they obtained the desired insertions ...

  7. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...synthetase Mammalian Cells pCMV-DnpK dinitrophenyl hapten Mammalian Cells pANAP AnapRS Mammalian Cells ...pMAH-POLY Polyspecific aminoacyl-tRNA Synthetase Mammalian Cells pDULE-ABK pyrrolysyl-tRNA synthase for...for aliphatic diazirine amino acids E. coli and Mammalian Cells pEVOL-pAzF synthetase for p-azido-l-phenylalanine...tetracysteine tag. In addition, since these tags are so small, one can insert them in virtually any desired location...

  8. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...organism Yeast are single cell, eukaryotic organisms formally classified as fungi. They reproduce by either ...the 1970s with the goal of producing high protein animal feed. Due to increasing prices of methanol and ...glycosylated proteins. Peroxisome studies Peroxisomes are small, membrane-bound organelles essential for sequestering...proliferate upon a media change into methanol and peroxisomal autophagy can be induced by removing methanol... This feature makes the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation...

  9. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ...libraries have been created in lentiviral systems for mammalian screens, which enables researchers to maximize...functionally conserved, they develop rapidly, they are small, and their transparency allows for whole organism...accelerate the discovery of additional genes and small-molecule probes to aid in the understanding and ...Such voltage imaging can be performed in intact animals and offers many advantages over traditional measurements...recordings. However, technical difficulties such as small fluorescence changes or a requirement for very high...

  10. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...particles are often found with cellular exosomes, small membrane vesicles or microvesicles (approx. 40 -...interaction with AAVs (Schiller et al., 2018). Exosomal vesicles secreted from producer cells also contain...conventional AAVs (AAV preparations were always normalized to their genomic titers). Examples include the...target less accessible cells, such as brain tumor stromal cells in a mouse model of glioblastoma (Volak et...vector-mediated genetic modification of brain tumor stromal cells after intravenous delivery." Journal of neuro-oncology...

  11. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ... difficult to detect, and thus quantify, due to small fluorescence changes of the donor and acceptor after...spatio-temporal dynamics of apoptosis in living animals. To do this they modified a different self-assembling...fluorescence was dependent on protease activation. Normally, β10-11 are antiparallel. To prevent binding, ...the sequence, β10 and β11 can rearrange to their normal antiparallel position and fluoresce. FlipCherry... a beta strand of GFP for imaging apoptosis in animals." Journal of the American Chemical Society (2019...

  12. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...out of their optimally suited temperature ranges and make them grow better at suboptimal ones, which could...are your regular next-door microbes which grow optimally in moderate temperatures (usually defined between...worth the trouble.  Enter ELSA: E. coli Learning Suboptimal Acclimatisation To resolve this impasse, our ...mesophilic chaperones would not function well under suboptimal conditions. This means recombinant proteins can...

  13. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...hybridomas come from animals that were immunized against the target protein. Animals are first injected ...injected with a small peptide that represents a short, unique amino acid sequence found in the protein, or, are.... After isolating the B cells from an immunized animal, those B cells are fused to myeloma cells at a ...develop a hybridoma than it does to immunize an animal and recover polyclonal antibodies (Lipman et al...

  14. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...chromatin at the level of nucleosomes, loops, chromosomal domains, or how stretches of DNA on different...our collection; the following articles are just a small cross-section spanning 20 years of research. DNA...Chromosomes. Samwer M, Schneider MWG, Hoefler R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW Cell. 2017 Aug...Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents. Yamagata...

  15. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...reported titer by either method so to ensure equal animal dosing, we advise that users keep titering method...per mouse to get phenotypes (that’s almost 100ul/animal for the PHP.eBs that we have in house!), which ... injection volumes with your IACUC committee.  Animal Models Q9: Is there any difference in transfection...to the development of neutralizing antibodies in animal models. All of our engineered AAV are presently...creative ways. The continued development of more and smaller CRISPR tools is an exciting area for future AAV...

  16. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ... next steps in the process involve repairing chromosomal DSBs. It is important to know that cells possess...generated in the course of repair by NHEJ are typically small (1-10 bp) but extremely heterogeneous. There is ...cleavage, which may interfere with loading of Ku and normal NHEJ activity. Other factors can also influence...EA, Giovannangeli C, Jasin M, Brunet E (2014) Chromosomal Translocations in Human Cells Are Generated by...Repair CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video] Pooled CRISPR Libraries Offer...

  17. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...mentees who meet together for an 8 month formal program. After the formal program ends, some 50% of these groups...supporters and advisors? I tried to start a more formal mentoring program at my company, but there weren't...awkward to ask someone for the time commitment of a formal mentoring relationship or there aren’t enough senior...for one another (trainees never get to present formally often enough) and give honest, critical feedback...

  18. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...be used to express genes of interest in cell and animal models. AAVs are non-pathogenic, do not integrate...hand, if you’re interested in expressing multiple small genes, you can design single rAAV vectors with multiple...viral cargo is usually quite stable and in some mammalian tissues has shown expression for several years...host genome and therefore the transduced gene is episomal and does not divide and replicate in the same ...2013.   scAAV If your gene of interest is truly small (<2.5Kb) and you need expression in a hurry, self-complementary...

  19. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    ...ubiquitin ligase, is the basis of TRIM-away. In normal physiology, TRIM21 recruits ubiquitin and the proteasome...tested multiple methods for TRIM-Away delivery. For small cell populations, co-microinjection of TRIM21 protein... et al. Cell. 2017. PubMed PMID: 29153837. Endosomal pH sensors for STED nanoscopy Article contributed...contributed by Eric J. Perkins Listen to Endosomal pH Sensors podcast segment The first Addgene plasmid deposit...using conventional light microscopy due to their small size (<250 nm), and studying endocytosis is further...

  20. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...observed abnormal cell migration and contraction. The lab hypothesized that this is abnormally deregulated... It is estimated that CTD is the second cause of male intellectual disability after Fragile X. There is... collection to perform a functional screen in mammalian cells to identify any solute channels that can...team will be able to validate their findings in mammalian cells with both human and murine targets thus ...
Showing: 241 - 260 of 809 results