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Showing: 241 - 260 of 740 results
  1. Supporting Reproducibility with a Connected ELN

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    Blog Post
    ... for reproducibility than others. Unfortunately, most ELNs are designed as closed ecosystems and act as...graphic depicts, RSpace supports linking to all of the most commonly used online file storage tools, as illustrated...depicted in the graphic, RSpace is integrated with most of the tools noted above, and has a modern API. ... used and/or referenced in the experiment. Since most ELNs, unlike data repositories, are designed to ...
  2. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    Blog Post
    ...non-integrating RNA virus Episomal vectors- extrachromosomally maintained DNA plasmids that express reprogramming...been used successfully to derive iPSCs from PBMCs. Most laboratories use Sendai virus to reprogram PBMCs...encoding reprogramming factors behave as separate extrachromosomal elements in the nucleus of the target cell...colonies are obtained by 10 days after electroporation, most of which are integration-free, karyotypically normal...
  3. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post
    ...off-target breaks, almost always resolved without any toxic effects to the cell. This means almost no indels and...retargeted. This finding was surprising – in the most common RNA targeting system, Cas13, the "collateral...one-size-fits-all approach to gene replacement therapy for most patients. But how does one go from “cut and paste...
  4. Mesothelioma - Causes, Symptoms, and Treatment

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    Blog Post
    .... Such misdiagnosis and late discovery mean that most mesothelioma patients have an extremely poor prognosis...question that asbestos exposure remains the single-most important factor in developing mesothelioma. Asbestos...to spread beyond its point of origin. One of the most exciting developments recently is a new blood test...for mesothelioma are creeping upwards, and while most people still die within a year of diagnosis, the...
  5. Using Video to Share Your Science: We Share Science

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    Blog Post
    ...such as finding time to read all of these articles. Most of us hardly have time to keep up with all the research...science. TedTalks are very popular, though they focus mostly on highly innovative ideas rather than on the day-to-day...in 39 countries, and in over 54 languages. While most scientific videos were for entertainment or basic...from any discipline are welcome. Up to this point most videos are based on research that has been published...
  6. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post
    ...distribute several of our most popular CRISPR pooled libraries and one of our most popular barcoding libraries... cDNA, barcoding, screening, and gRNA libraries. Most of the pooled libraries at Addgene are lentiviral...thaw cycles, we include a 1.3 mL aliquot with mostlibrary requests specifically to allow scientists to ...
  7. Antibodies 101: Immunoprecipitation

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    Blog Post
    ...protein is then eluted. This method is appropriate for most situations.  A slightly different method is desirable...antibody to bind to the target protein. One of the most common immobilization methods utilizes bacterial...Consequently, users need to choose the protein type that is most compatible with the host species and isotype of ...Protein A/G, was developed, which can be used for most antibodies compatible with either Protein A or G...
  8. The Challenges of Cell Culture

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    Blog Post
    ...Special Collections). Arguably, oncology has benefited most as cancer cells are amenable to culture in vitro...about since 1966 when Stanley Gartler reported that most of the 18 cell lines he had studied (the majority...lines suffer from contamination with HeLa being the most frequent contaminant, closely followed by T24 bladder...their original description. The oldest, largest, and most diverse of these repositories is the American Type...
  9. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post
    ...essential. In this article, we’ll go through some of the most important considerations to make when planning and... tissue sample where they bind to their targets, most commonly proteins. You can then use fluorescent ...choosing a primary, so we’ll start there. Some of the most important considerations are to make sure your secondary...technique can provide you not only answers to your most pressing research questions, but also stunning images...
  10. 10 Steps to a Perfect Science Talk

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    Blog Post
    ... even great, speaking skills can be attained by almost anyone with enough practice. I always take a poll...a lot of data.  I can’t say this enough…more is almost never better. 10. Be enthusiastic You don’t have...possible, practice enough so that you can deliver most of your talk without notes. Don’t be hard on yourself... “and who has deposited to the repository?” When most or all of the hands go down it is a great segue ...
  11. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post
    ... be treated with “one-size-fits-most” NHEJ-mediated editing, but most other diseases will require HDR-...Collections Pages Duchenne muscular dystrophy: The most common inherited disease The genetic disease targeted...editing used in the exon skipping approaches here. In most genetic diseases, the mutational landscape is broad...
  12. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ...composed of an n-terminal DNA recognition domain and a mostly c-terminal nuclease domain. Feng Zhang's group ...untranslated in the nucleus, confirming the hypothesis that most mRNAs are not translated until they are exported...mammalian cells. Multicolor CRISPR labeling chromosomal loci in genome Thoru Pederson’s lab at the University...dynamic interactions between intra- and inter-chromosomal domains. The authors anticipate that this tool..., genome rearrangements and cancer-associated chromosome shattering. This method is based on the recognition...intra-nuclear distance between loci on different chromosomes. The advantage of having this kind of system ...can be difficult to compare their performance, as most translation initiation sequences are optimised for...
  13. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post
    ...SpCas9, with a PAM preference of NGG, is still the most widely-used Cas enzyme, the development of SaCas9... knockout via CRISPR cutting. For CRISPRa, it is most-efficacious to target a ~100nt window upstream of... a ~100nt window downstream of the TSS gives the most activity. Thus, a given gene will only have a dozen...CAGE-seq to directly capture the mRNA cap, provides the most accurate mapping (Radzisheuskaya et al., 2016). ...
  14. Plasmids 101: Repressible Promoters

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    Blog Post
    ...generation Tet-Off vectors (Tet-Advanced)” are the most recent variants of the Tet-Off system.  For more...post contains only a brief overview of some of the most common repressible promoter systems, but there are... PMC3574576. Lai, Sen-Li & Tzumin Lee. “Genetic mosaic with dual binary transcription systems in Drosophila... for transgene expression, lineage tracing, and mosaic analysis.” Cell 141(3) (2010): 536-48. PubMed PMID...
  15. CRISPR 101: Off-Target Effects

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    Blog Post
    ...essential to understand them in order to design the most specific assays possible. In this blog, we will ...tools generating a prediction for off-target levels, most of them will also give you a specific list of the... Graphic courtesy of Maya Kostman. The most commonly used Cas, spCas9, has gone through multiple...exact gRNA matches (Rabinowitz and Offen). Of note, most of these optimized Cas enzymes prevent cutting at...
  16. A Control for All Seasons

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    Blog Post
    ...Broadly useful controls Positive and negative controls Most biological experiments require positive and negative...the experimental group.     Figure 1: A, Most biological experiments will benefit from positive...that must be considered (Burry 2011). Perhaps the most critical are those that confirm primary antibody...example at sites of inflammation. This tends to be most problematic when staining cells or tissues that ...
  17. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    Blog Post
    ...but I didn’t know what level of expression was the most efficient for gene editing. A virus encoding GFP... practice you would select the Cas9 line that’s “most efficient at editing” to introduce the gRNA, but...regions of the gene with multiple gRNAs, but the most important is that you could avoid potential off ...sites targeted by my specific gRNAs (Figure 4). The most difficult part of the assay was designing primers...
  18. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    Blog Post
    ...recombinases. How might one read the state of an RSM? The most straightforward way to read the state of an RSM ...disadvantages. Sanger sequencing is simple and the most accurate method as it directly reads the state of...that we defined in our paper. In our opinion, the most convenient strategy is the fluorescent reporter ...output plasmid (single copy, bacterial artificial chromosome backbone) contains the register. Depending on...
Showing: 241 - 260 of 740 results