We narrowed to 766 results for: ncl

...current genome engineering applications of CRISPR including: generating single or multi-gene knockouts in ...) that harbors two surface exposed cysteines, including the native C547, allowing for ligation to pyridyl...Senior Scientist at Addgene.  Andrew's interests include genome engineering and microbiology.     References...

... different.  Conclusion Figure 7: Mean inferred effects by region. In conclusion, our model infers...requests correlate with paper publication The dataset includes 11,426 plasmid requests over 6 years for 35 papers...

...mind that access and availability to resources (including the internet!) can vary depending on factors like...your best to understand what kind of resources - including your time - will be required to produce an outcome...naked eye or to make things more accessible by including, highlighting, or omitting details. Digital or...

... Selecting crispr in Guides will return links including our CRISPR guide.  For a closer look at this new... deposit prior to publication and were able to include specific plasmid ID numbers. If you know the ID...suggest direct links to other types of searches including depositing labs and institutions. For example,...

...proposed as a mechanism for increasing diversity and inclusion in STEM disciplines (Bangera & Brownell, 2014;...promote professional success (many of my students include their in-class research experience on their resumes...experiences can make scientific research more inclusive. CBE Life Sciences Education. 13(4):602-606. Brian...

...viral vectors in the lab. Advantages of lentivirus include a large genetic capacity and the ability to transduce...CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested plasmid in 2015: lentiCRISPR... observed in small trials for other diseases, including the common hematopoietic disorders sickle cell...

... and management process or the whole process, including grant readiness, funding opportunity research,...grant training, mentoring and coaching services, including Smartegrants, to help those entering the Grants...LLC, a grant professional development firm that includes the social media community GrantChat. She has ...

...scientists employ GFP for a wide number of functions, including: tagging genes for elucidating their expression...detect a variety of intracellular conditions, including ion (such as Ca2+) concentrations and pH, using...selection: Expression constructs like plasmids often include GFP as a marker to help identify which cells have...

...If you choose this strategy, it’s important to include the proper protein expression elements (ribosome...entry clones available for many popular genes, including Hs.KRAS4a. Use our website to search for your ...Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple Lentiviral Expression Systems...

...the CRISPR workflow. For a multiplex sample, ICE includes visual representations of all detected edit types...indel.   For a complete guide to ICE analysis, including detailed explanation and examples, read the paper...stack Genome Engineering Solutions, which also includes our CRISPR knockout guide design tool, guide validation...

...proteins. Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin...translation of an array of different reporters including mScarlet-I, luciferase, antibiotic resistance ...can be used for an array of detectable proteins including fluorescent proteins, antibiotic resistance, and...

... bioinformatics pipelining In many use-cases, including ours at Addgene, the overall purpose of a data...workflow management system for scientific pipelines, including bioinformatics. We loved the parallels between...language.  The modern architecture of Nextflow included built-in support for containers, such as Docker...

... hardest part of any revolutionary discovery, including CRISPR, is turning potential into impact. In molecular...measurable cell toxicity – using Cas7-11. This included tests done in cell lines known to be more sensitive... — the ability to now make any small changes, including base edits, insertions, and deletions. But CRISPR...

...spacers of the crRNA can be customized as well, including concatenations into crRNA arrays targeting multiple...by sacB counterselection.  The MUCICAT toolkit includes versions of plasmids with different induction ...concentration. Image from the Yang lab.   In conclusion, the MUCICAT system has the following advantages...

...contain components of the SB transposon system including those from the original publication, Ivics et ... been studied in many contexts within the fly including oogenesis and cytokinesis. Why name this protein...that contains bagpipe from the Deplancke lab. Conclusion I hope you enjoyed this tour of Addgene’s new...

...many types of pooled libraries here at Addgene, including cDNA, barcoding, screening, and gRNA libraries...viruses are sensitive to freeze/thaw cycles, we include a 1.3 mL aliquot with mostlibrary requests specifically...aliquot to full use! Common conditions to optimize include incubation time, antibiotic conditions, and amount...

...(GTA). The GTA has a wealth of information and includes annotated images of the brain sections describing...enhancers and their neuroscience research. This includes other data, tools, and resources. Luckily for ...are also more resources available at Addgene, including the Allen Institute for Cell Science Plasmid Collection...

...Don’t dive directly into the pipetting! MoClo nomenclature and structure can be quite confusing for new...These overhangs are known as the common MoClo nomenclature. The overhangs are generated using type IIS ... your cloning. Most sequence analysis software includes Golden Gate cloning tools that simulate the restriction–ligation...

...N-glycosylase (UNG) enzymes. Various other proteins included in different CGBE tools allow the researcher to...again, this post has a lot of acronyms, so we've included a glossary at the end of this post! Figure...for their application from among many options.  Conclusion With these advances, every base can now be directly...

...rather late stages (i.e. postdoc level) and can include the pressure to publish in high-impact journals...for insights from my time in technical support including tips, advantages, and disadvantages of this overall...without being too commercial or “sales-y”. The work includes reviewing customer data sets, troubleshooting ...