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We narrowed to 457 results for: nsf

Showing: 241 - 260 of 457 results

  1. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...million.  After making the dilutions, they are transferred to a new plate containing the mastermix which...generator, you will have to be careful about transferring your droplets from the droplet generator to ...

  3. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...AAV) vectors are the most frequently used gene-transfer tools in the study of the brain and spinal cord...selection yields AAV variants for widespread gene transfer to the adult brain. Nature biotechnology. 2016...

  4. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...pattern opens up the possibility of directional gene transfer, analogous to traditional restriction enzyme cloning... cloning. Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells...

  5. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...can use your cover letter to explain why your transferable skills make you a good candidate for the position...experience or skills, often by pointing to your transferable skills. In the above example, if you don’t have...

  7. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...Lentiviral production uses three plasmids: (1) The transfer plasmid, (2) the envelope plasmid, and (3) the...the packaging plasmid. These are transfected into target cells which produce lentiviral particles.   ...

  8. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ... if these do not provide clarity, a wet lab transformation experiment will ultimately inform whether your...classification and identification based on replication and transfer systems and host taxonomy. Frontiers in Microbiology...

  10. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...are referred to as methods of ‘horizontal gene transfer’. Resistance can also arise from random mutations...either through random mutation or horizontal gene transfer, means to defend themselves from these naturally...

  13. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...biosensors. Aptabeacons Fluorescence resonance energy transfer (FRET) technology has been studied and used in...fluorescence-activated cell sorting (FACS) of E. coli transformed with aptamer libraries, the Jaffrey group selected...

  14. How to Write a Scientific Review Article

    Type
    Blog Post
    ...drafts of the manuscript. This is the time to transform the document into something cohesive-- change ...Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell ...

  15. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...site. (c) The pCAG-EGxxFP target plasmid was co-transfected with pX330 plasmids containing sgRNA sequences...efficacy of your gRNAs is then a simple matter of transfecting HEK293T cells with your modified pCAG-EGXXFP...

  16. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...delivered as any type of small RNA and can occur via transfection or viral delivery of a plasmid. RNAi is an umbrella...mature siRNA. siRNAs are delivered via transient transfections and are handy for quick in vitro experiments...

  17. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...second-generation system as an example.  The lentiviral transfer plasmid encodes your insert of interest, flanked...facilitates viral entry into the host cell.  Transfecting these three plasmids into cells enables the ...

  18. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...as effective NIR fluorescence in transiently transfected live HeLa cells with no supply of exogenous BV...and after normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0...

  19. Five Popular Model Organisms

    Type
    Blog Post
    ...Efficient gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming sequences...

  20. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...aptamers are used to track cellular processes by transforming cells with plasmids encoding aptamer-tagged ...expression vectors to create a library. Bacteria were transformed, and then sorted by fluorescence-activated cell...
Showing: 241 - 260 of 457 results