We narrowed to 304 results for: pET
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TypeBlog Post... PMC4927352. Janetopoulos, Chris, Tian Jin, and Peter Devreotes. "Receptor-mediated activation of heterotrimeric...
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Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...mutation. Here, the blocking-only templates will compete with the blocking/intended mutation templates resulting... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post... E127-E130. PubMed PMID: 11988757. 2. Hornbeck, Peter V., et al. "PhosphoSitePlus, 2014: mutations, PTMs... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells... -
Lentiviral Vector Guide
TypeGuide...improvements and the potential for replication-competent lentivirus production — that is, creation of viral...usually encodes for VSV-G Safety Safe; replication incompetent by using three separate plasmids encoding the...the necessary viral genes Safer; replication incompetent by using four plasmids instead of three and eliminates...: The potential for generation of replication-competent lentiviral vectors The potential for oncogenesis...oncogenesis The potential for generation of replication-competent lentiviral vectors is very low thanks to the design...to alter infectivity and tropism. Replication-competent The ability of a virus (or viral vector) to replicate... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...including 129 and C57BL/6, for which we have germline competent ESC lines. Second, the process is much quicker... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post...transposase (PBx), which contains mutations making it competent for excision, but defective for integration [4... -
Finding Your Perfect Job After University
TypeBlog Post...obtaining work in scientific research would be very competitive, and so I applied for a summer role as a Research... -
Adeno-associated virus (AAV) Guide
TypeGuide...formation of replication-competent viruses. While AAV themselves are replication incompetent, there is a rare ...plasmids could lead to the production of replication-competent viruses that would be able to infect cells and...information. Viral Vector Safety AAVs are replication incompetent and not known to cause disease in humans. Most...The risk of spontaneously occurring replication-competent rAAV vectors is extremely reduced by dividing ...deliver genetic material into cells. Replication-competent rAAV Mutated rAAV vector that has the ability ... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post... is a transposon? DNA transposons are mobile, repetitive genetic elements that are found in both prokaryotic... -
Finding Your Science Policy Path
TypeBlog Post...system? Defining your why is what will make you a competitive candidate for any science policy position and... -
A History of Genome Engineering in Popular Culture
TypeBlog Post...successful in the box office, indicating the insatiable appetite of the moviegoers for dinosaurs wreaking havoc... -
Adenovirus Guide
TypeGuide...genome could lead to the production of replication-competent adenoviruses (RCAs) that would be able to infect...oncolytic rAdV vectors, which are replication-competent adenoviral vectors that can selectively infect...Recombinant adenoviral vectors, although replication incompetent, are human pathogens and have to be handled at...adenovirus (GLAd) free of adenovirus and replication-competent adenovirus (RCA) contaminants . Experimental &...deliver genetic material into cells. Replication-competent adenovirus (RCA) Mutated adenoviral vector that... -
Antibodies 101: Flow Cytometry
TypeBlog Post...keep samples on ice at all times and use gentle pipetting instead of vortexing. Include proper controls ... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post... However, many scientists operate in a hyper-competitive environment where getting hired hinges on performing... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...attached fluorophore. However, since tRNA suppression competes with natural chain termination at stop codons,... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post... propagated in DH5α. For plasmids with highly repetitive sequences (which can be prone to recombination... -
Gamma-Retroviral Vector Guide
TypeGuide... to reduce the chance of creating replication-competent retroviruses. For a summary of all gamma-retroviral...: The potential for generation of replication-competent gamma-retroviral vectors The potential for oncogenesis...oncogenesis The potential for generation of replication-competent gamma-retroviral vectors is very low thanks to...to alter infectivity and tropism. Replication-competent The ability of a virus (or viral vector) to replicate... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post... the truncated pegRNA can still guide Cas9 and compete with full pegRNAs for access to the target site...