We narrowed to 303 results for: pET
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  TypeBlog Post...for a Faculty Position" eBook Here 1. Have a competitive CV You don't necessarily need a Nature/Cell/Science...
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  Using Phosphoserine to Study Protein PhosphorylationTypeBlog Post... E127-E130. PubMed PMID: 11988757. 2. Hornbeck, Peter V., et al. "PhosphoSitePlus, 2014: mutations, PTMs...
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  Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of NeuronsTypeBlog Post...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...
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  CRISPR GuideTypeGuide...have been used for dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and...labeled proteins to the site. Chromosome-specific repetitive loci can be efficiently visualized in living ...multiple target sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering...924–927. PMID: 30377362 Casini, A., Olivieri, M., Petris, G., Montagna, C., Reginato, G., Maule, G., Lorenzin... N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee, M. J., & Joung, J. .... Cell . 184 (9), 2503-2519.e17. PMID: 33838111 Peters, J. M., Koo, B., Patino, R., Heussler, G. E., Hearne... S., Silvis, M. R., Harden, M. M., Osadnik, H., Peters, J. E., Engel, J. N., Dutton, R. J., Grossman, ...
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  Generating Mouse Models Using CRISPR/Cas9TypeBlog Post...including 129 and C57BL/6, for which we have germline competent ESC lines. Second, the process is much quicker...
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  PiggyBac-ing Through the Genome Editing FieldTypeBlog Post...transposase (PBx), which contains mutations making it competent for excision, but defective for integration [4...
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  Finding Your Perfect Job After UniversityTypeBlog Post...obtaining work in scientific research would be very competitive, and so I applied for a summer role as a Research...
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  Lentiviral Vector GuideTypeGuide...improvements and the potential for replication-competent lentivirus production — that is, creation of viral...usually encodes for VSV-G Safety Safe; replication incompetent by using three separate plasmids encoding the...the necessary viral genes Safer; replication incompetent by using four plasmids instead of three and eliminates...: The potential for generation of replication-competent lentiviral vectors The potential for oncogenesis...oncogenesis The potential for generation of replication-competent lentiviral vectors is very low thanks to the design...to alter infectivity and tropism. Replication-competent The ability of a virus (or viral vector) to replicate...
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  Adeno-associated virus (AAV) GuideTypeGuide...formation of replication-competent viruses. While AAV themselves are replication incompetent, there is a rare ...plasmids could lead to the production of replication-competent viruses that would be able to infect cells and...information. Viral Vector Safety AAVs are replication incompetent and not known to cause disease in humans. Most...The risk of spontaneously occurring replication-competent rAAV vectors is extremely reduced by dividing ...deliver genetic material into cells. Replication-competent rAAV Mutated rAAV vector that has the ability ...
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  Finding Your Science Policy PathTypeBlog Post...system? Defining your why is what will make you a competitive candidate for any science policy position and...
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  A History of Genome Engineering in Popular CultureTypeBlog Post...successful in the box office, indicating the insatiable appetite of the moviegoers for dinosaurs wreaking havoc...
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  Plasmids 101: Using Transposons in the LabTypeBlog Post... is a transposon? DNA transposons are mobile, repetitive genetic elements that are found in both prokaryotic...
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  Adenovirus GuideTypeGuide...genome could lead to the production of replication-competent adenoviruses (RCAs) that would be able to infect...oncolytic rAdV vectors, which are replication-competent adenoviral vectors that can selectively infect...Recombinant adenoviral vectors, although replication incompetent, are human pathogens and have to be handled at...adenovirus (GLAd) free of adenovirus and replication-competent adenovirus (RCA) contaminants . Experimental &...deliver genetic material into cells. Replication-competent adenovirus (RCA) Mutated adenoviral vector that...
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  With an Eye Towards the Future, We Look Back at the March for ScienceTypeBlog Post... However, many scientists operate in a hyper-competitive environment where getting hired hinges on performing...
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  Antibodies 101: Flow CytometryTypeBlog Post...keep samples on ice at all times and use gentle pipetting instead of vortexing. Include proper controls ...
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  Special Delivery: Fluorophore Targeting for FRET StudiesTypeBlog Post...attached fluorophore. However, since tRNA suppression competes with natural chain termination at stop codons,...
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  How to Deposit Your Plasmids with AddgeneTypeBlog Post... propagated in DH5α. For plasmids with highly repetitive sequences (which can be prone to recombination...
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  Gamma-Retroviral Vector GuideTypeGuide... to reduce the chance of creating replication-competent retroviruses. For a summary of all gamma-retroviral...: The potential for generation of replication-competent gamma-retroviral vectors The potential for oncogenesis...oncogenesis The potential for generation of replication-competent gamma-retroviral vectors is very low thanks to...to alter infectivity and tropism. Replication-competent The ability of a virus (or viral vector) to replicate...
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  Prime Editing: Adding Precision and Flexibility to CRISPR EditingTypeBlog Post... the truncated pegRNA can still guide Cas9 and compete with full pegRNAs for access to the target site...
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  The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your NeedsTypeBlog Post...speed up the sgRNA design process by removing repetitive tasks and/or by providing features to help with...