We narrowed to 304 results for: pET
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TypeBlog Post...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...
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3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...mutation. Here, the blocking-only templates will compete with the blocking/intended mutation templates resulting... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post... E127-E130. PubMed PMID: 11988757. 2. Hornbeck, Peter V., et al. "PhosphoSitePlus, 2014: mutations, PTMs... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...including 129 and C57BL/6, for which we have germline competent ESC lines. Second, the process is much quicker... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post...transposase (PBx), which contains mutations making it competent for excision, but defective for integration [4... -
Finding Your Perfect Job After University
TypeBlog Post...obtaining work in scientific research would be very competitive, and so I applied for a summer role as a Research... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post... is a transposon? DNA transposons are mobile, repetitive genetic elements that are found in both prokaryotic... -
A History of Genome Engineering in Popular Culture
TypeBlog Post...successful in the box office, indicating the insatiable appetite of the moviegoers for dinosaurs wreaking havoc... -
Finding Your Science Policy Path
TypeBlog Post...system? Defining your why is what will make you a competitive candidate for any science policy position and... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post... However, many scientists operate in a hyper-competitive environment where getting hired hinges on performing... -
Antibodies 101: Flow Cytometry
TypeBlog Post...keep samples on ice at all times and use gentle pipetting instead of vortexing. Include proper controls ... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...attached fluorophore. However, since tRNA suppression competes with natural chain termination at stop codons,... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post... propagated in DH5α. For plasmids with highly repetitive sequences (which can be prone to recombination... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post... the truncated pegRNA can still guide Cas9 and compete with full pegRNAs for access to the target site... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post...speed up the sgRNA design process by removing repetitive tasks and/or by providing features to help with... -
Delivery Methods for Generating iPSCs
TypeBlog Post...of using Sendai is that since it’s replication competent, it’s difficult to eliminate the virus from all... -
CRISPR Guide
TypeGuide...have been used for dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and...labeled proteins to the site. Chromosome-specific repetitive loci can be efficiently visualized in living ...multiple target sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering...924–927. PMID: 30377362 Casini, A., Olivieri, M., Petris, G., Montagna, C., Reginato, G., Maule, G., Lorenzin... N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee, M. J., & Joung, J. .... Cell . 184 (9), 2503-2519.e17. PMID: 33838111 Peters, J. M., Koo, B., Patino, R., Heussler, G. E., Hearne... S., Silvis, M. R., Harden, M. M., Osadnik, H., Peters, J. E., Engel, J. N., Dutton, R. J., Grossman, ... -
Lentiviral Vector Guide
TypeGuide...improvements and the potential for replication-competent lentivirus production — that is, creation of viral...usually encodes for VSV-G Safety Safe; replication incompetent by using three separate plasmids encoding the...the necessary viral genes Safer; replication incompetent by using four plasmids instead of three and eliminates...: The potential for generation of replication-competent lentiviral vectors The potential for oncogenesis...oncogenesis The potential for generation of replication-competent lentiviral vectors is very low thanks to the design...to alter infectivity and tropism. Replication-competent The ability of a virus (or viral vector) to replicate... -
Adeno-associated virus (AAV) Guide
TypeGuide...formation of replication-competent viruses. While AAV themselves are replication incompetent, there is a rare ...plasmids could lead to the production of replication-competent viruses that would be able to infect cells and...information. Viral Vector Safety AAVs are replication incompetent and not known to cause disease in humans. Most...The risk of spontaneously occurring replication-competent rAAV vectors is extremely reduced by dividing ...deliver genetic material into cells. Replication-competent rAAV Mutated rAAV vector that has the ability ...