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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 563 results for: pac

Showing: 241 - 260 of 563 results

  1. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...Running your transfer in the cold room or packing the box with ice packs can help keep the system cool, as can...overheating. Try running it in a cold room or putting ice packs in and/or around the gel box. If overheating remains...

  4. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful for producing...difficult-to-fold proteins. In addition, P. pastoris has the capacity to glycosylate proteins and may recapitulate the...

  9. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...4D nucleome, the organization of the nucleus in space and time, and to understanding how nuclear organization...that they could evaluate the degree of chromatin compaction even for this small distance! To the authors’...

  12. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...the correct solution is fundamental, and it will impact the time you will be able to store your sample .... The two solutions may differ though in their capacity to maintain DNA stability during storage over ...

  13. Fostering communication in remote collaborations

    Type
    Blog Post
    ...feel included in the project and accelerates the pace at which problems can be addressed and new ideas...that work best for them. This also accelerates the pace of research by circumventing the slower, sequential...

  14. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ..., and disease. In order to expand their model’s impact, organoid users have recently begun to develop ...these novel biosensors could have a major economic impact.   Design and mechanism of action of the...

  15. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...experiment and the organism you are working in. The spacing of genes and whether or not it’s ok to disrupt ...the known and unknown microbial coding sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org/10.7554...

  16. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...add the citation and release the hold. Increasing impact Making the plasmids available as soon as your paper...paper is published helps maximize the potential impact of your work. Plus, it’s costly for us to process...

  17. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...repository! This includes the first Addgene prep packaged in the AAV9-X1.1 serotype.  In rodents, AAV9-...You can now target these cells with AAV-CAG-GFP packaged in AAV9-X1.1!  We also have eight new AAV-PHP....

  18. An Addgene Summer Internship

    Type
    Blog Post
    ...first week at Addgene, I had to get used to the workspaces, as well as complete the lab safety and cybersecurity...safety, general work skills — such as using Google workspace — and got the chance to work on my very own research...

  19. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...aliquots in a kit with a huge amount of wasteful packaging.  Turns out, one could make a lifetime supply ...indefinitely at room temperature (thus also saving freezer space).  The procedure becomes less attractive when noting...

  20. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...blockbuster known as the CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes...genetic material next to the species-specific protospacer adjacent motif (PAM). Once the CRISPR/Cas complex...
Showing: 241 - 260 of 563 results