We narrowed to 353 results for: plat

1. All in a Twist: dsRNA

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   Blog Post

   ...During transcription, RNA is synthesized from a DNA template, inherently generating RNA:DNA intermediates in...

2. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   Blog Post

   ... electroporated cells re-adhered to the culture plate within 4 hr of treatment. In some primary cell lines...

3. RANbodies: Reporter Nanobody Fusions

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   Blog Post

   ...individual labs to complete. The nanobody based RANbody platform from the Sanes Lab overcomes this limitation and...

4. What's New in CRISPR - November 2021

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   ...other prime editing tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable...

5. RaPID Detection of RNA-protein Interactions

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   Blog Post

   ...interactions with RaPID RaPID is similar to the BioID platform for identifying protein-protein interactions, ...

6. dTAG - You're it!

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   Blog Post

   ...FKBP12F36V-tagged proteins using our lentiviral expression platform, to assess selective degradation of FKBP12F36V-BRD4...

7. Seven Tips for Using LinkedIn as a Scientist

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   Blog Post

   ...volunteer organizational work. LinkedIn is not the platform for politics, family updates, or click bait of...

8. Working with Nuclear Receptors

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   Blog Post

   ... The development of the transient transfection platform for assaying transcriptional activity, for example...

9. Making CRISPR Plasmids Using Fragmid

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   Blog Post

   ...cloning, perform an exonuclease cleanup, transform, plate, and pick. In short, the Fragmid system takes the...

10. Plasmids 101: Modular Cloning

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    Blog Post

    ... them to these organisms via another optimized platform. Gene Stacking As the name implies, gene stacking...

11. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    Blog Post

    ...Other” column. For example, you may enter “donor template vector”. Cloning Method Choose from: Gateway...

12. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post

    ... MET genes to grow.) Students then use replica plating to determine if expression of MET/Met genes from...

13. Early Career Researcher Toolbox: Finding Relevant Papers

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    Blog Post

    ...the topic.  Meta Meta is a literature discovery platform developed by the Chan Zuckerberg Initiative. Meta...

14. Hot Plasmids and Viral Preps - March 2021

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    Blog Post

    ...assembly By Eric Perkins Though it’s easy enough to contemplate DNA in terms of its sequencing, in vivo studies...

15. Popular Retroviral Vectors and Their Uses in Scientific Research

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    Blog Post

    ...Consortium, also known as the Genetic Perturbation Platform, to produce their shRNA library to support functional...

16. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    Blog Post

    ... of Glycosylation (APCDG) and an international platform for CDG research, named the CDG&Allies – Professionals...

17. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    Blog Post

    ... WA (2013) Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression...

18. Cpf1 Update: Comparison to Cas9 and NgAgo

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    Blog Post

    ...mechanism. To prevent new editing post-HDR, repair templates should remove the PAM sequence. Many research ...

19. Plasmids 101: Terminators and PolyA signals

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    Blog Post

    ...100% efficient at halting transcription of the template and initiating the desired cleavage event, although...

20. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post

    ... S., & Ng, S. K. (2021). HEK293 cell line as a platform to produce recombinant proteins and viral vectors...