...most commonly used is the Illumina NGS platform. This is the platform used by Addgene’s sequencing partner...of DNA, or templates, are spatially separated from each other. These attached DNA templates are then amplified... again producing ~1,000 copies of each template. Each template is then replicated using the modified bases...antibiotic(s) to add to your LB media or LB agar plates. Antibiotic Recommended Stock Concentration Recommended...the 4 nucleotides, a DNA polymerase enzyme, the template DNA to be copied, and a primer. A primer is a ...is a high-throughput, multi parallel sequencing platform that can generate sequencing data for up to 600...normal” bases in the reaction. All the sequenced templates are then aligned to each other to assemble the...

... to utilize HDR for gene editing, a DNA repair template containing the desired sequence must be delivered...the gRNA(s) and Cas9 or Cas9 nickase. The repair template must contain the desired edit as well as additional...arms. Depending on the application, the repair template may be a single-stranded oligonucleotide, double-stranded...double-stranded DNA plasmid. When designing the repair template, do not include the PAM sequence present in the...the genomic DNA. This step prevents the repair template from being a suitable target for Cas9 cleavage..., you could alter the PAM sequence in your HDR template with a silent mutation that does not change the...this feature, a Cas13a-based molecular detection platform, termed SHERLOCK, has been used to differentiate...

... Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that uses standard curves to quantify...Overview of application Coat antigens to the well of a plate, either by charge interactions (direct) or by a ...several wash steps. Add primary antibody to the plate. After incubation, wash to remove unbound antibody...Activate the conjugate and read the output via a plate reader. Special considerations The two most common...of interest is attached to the well of a 96-well plate. A conjugated primary antibody is used to bind to...be sorted (separated and deposited into tubes or plate wells) by fluorescence activated cell sorting (FACS...

...produced. The antisense strand is referred to as the template strand or non-coding strand as this is the strand...promoter region stably and transcription of the template strand can initiate. The TATA box is a DNA sequence... is the RNA responsible for providing a stable template for the translation of a protein. RNA polymerase...

...High-efficiency blue-shifted channelrhodopsin from Platymonas subcordiformis 510 CoChR Channelrhodopsin from...